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Recombinant SARS-CoV-2 N Protein biotinylated(N-6His-Avi)

Uniprot : P0DTC2

Cat.No.:PKSV030311
Expression host: E.coli

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Description

Synonyms	SARS-CoV-2 coronavirus NP Protein;SARS-CoV-2 np Protein;SARS-CoV-2 novel coronavirus
Species	SARS-CoV-2
Expression_host	E.coli
Sequence	Met1-Ala419
Accession	QHD43423.2
Mol_Mass	51.3 kDa
AP_Mol_Mass	55-60 kDa
Tag	N-His-Avi
Bio_Activity	Immobilized 2019-nCoV NP Antibody (6G9)at 5μg/ml (100 μl/well) can bind 2019-nCoV Nucleocapsid Protein-His-Avi(PKSV030311). The ED50 of PKSV030311 is 92 ng/ml.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	Please contact us for more information.
Storage	Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation	Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 50mM Arginine, pH 7.5
Reconstitution	Not Applicable

Background

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

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