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Recombinant SARS-CoV-2 Spike RBD(N501Y)(His Tag)

  • Cat.No.:PKSV030352

  • Expression host: HEK293 Cells

To Purchase PKSV030352

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  • 100ug
Price: $1200
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Description

Species SARS-CoV-2
Expression_host HEK293 Cells
Sequence Arg319-Phe541(N501Y)
Accession YP_009724390.1
Mol_Mass 26.6 kDa
AP_Mol_Mass 35 kDa
Tag C-His
Bio_Activity 1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (mFc tag)(Cat: PKSH031870) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His(PKSV030352), the EC50 of PKSV030352 is 3-20 ng/mL. 2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) (PKSH031870) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein(PKSV030352) with an affinity constant of 0.827 nM as determined by Octet RED96e system.

Properties

Purity > 95 % as determined by SDS-PAGE.
Endotoxin level < 1.0 EU per μg protein as determined by the LAL method.
Storage Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, pH 7.4.
Reconstitution Please refer to the instraction in the hard copy of COA.

Background

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

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