Recombinant SMAC/Diablo Monoclonal Antibody (AN300074P)
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For research use only.
| Verified Samples |
Verified Samples in WB: K562, HeLa, Mouse heart, Rat skin in IHC: Human tonsil in IF: HeLa |
| Dilution | WB: 1:1000；IHC-P: 1:20；ICC/IF: 1:200-1:500；IP: 1:10-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P, ICC/IF, IP |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human SMAC / Diablo Protein |
| Abbre | DIABLO |
| Synonyms | DFNA, DIABLO, DFNA64, SMAC, 0610041G12Rik, DBLOH, DBOH, Diablo homolog, Diablo homolog (Drosophila), Diablo homolog mitochondrial, Diablo IAP binding mitochondrial protein, Diablo like protein, DIABLO S, Direct IAP binding protein with low pI, Direct IAP-binding protein with low pI, FLJ10537, FLJ25049, mitochondrial, Mitochondrial Smac protein, Second mitochondria derived activator of caspase, Second mitochondria-derived activator of caspase, SMAC 3, Smac protein, SMAC3 |
| Swissprot | |
| Calculated MW | 27 kDa |
| Observed MW |
21 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Cancer, Metabolism |
| Clone | A1184 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013] |
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