Recombinant SMARCA2/BRM Monoclonal Antibody (AN301908L)
For research use only.
| Verified Samples |
Verified Samples in WB: A549, HL-60, U-2 OS Verified Samples in IHC: Human lung Verified Samples in IF: 293, A549 |
| Dilution | WB 1:1000, IHC-P 1:200-1:2000, ICC/IF 1:50, FCM 1:200-1:500 |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC-P, ICC/IF, FC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | A synthetic peptide of human SMARCA2/BRM |
| Abbre | SMARCA2/BRM |
| Synonyms | SNF2L, SMARCA, BAF190B, BRM, SNF2A, SNF2L2, SMARCA2, ATP dependent helicase SMARCA2, ATP-dependent helicase SMARCA2, BAF190, BRG1-associated factor 190B, FLJ36757, Global transcription activator homologous sequence, hBRM, hSNF2a, MGC74511, Possible global transcription activator SNF2L2, Probable global transcription activator SNF2L2, Protein brahma homolog, SMCA2, SNF2 alpha, SNF2 like 2, SNF2/SWI2 like protein 2, SNF2-alpha, SNF2LA, Sth1p, Sucrose nonfermenting 2 like protein 2, SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 2, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 |
| Swissprot | |
| Calculated MW | 181 kDa |
| Observed MW |
190 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone | C211529 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | SMARCA2, also named as BRM, is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. |
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