Recombinant Streptomyces hygroscopicus Bar Protein

    • Recombinant Streptomyces hygroscopicus Bar Protein-Elabscience
    • Recombinant Protein-Elabscience
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    • Recombinant Streptomyces hygroscopicus Bar Protein-Elabscience
    • Recombinant Protein-Elabscience
    • Recombinant Streptomyces hygroscopicus Bar Protein-Elabscience
    • Recombinant Protein-Elabscience

      Catalog number:PKSQ050087

      Synonyms:Phosphinothricin N-acetyltransferase; PPT N-acetyltransferase; Phosphinothricin-resistance protein; bar

      Size:
      • 10μg
      • 50μg
      Qty:
      - +
      Price: $129

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      Overview

      Synonyms Phosphinothricin N-acetyltransferase; PPT N-acetyltransferase; Phosphinothricin-resistance protein; bar
      Species Streptomyces hygroscopicus
      Expression_host E.coli
      Sequence Met1-Ile183
      Accession P16426
      Mol_Mass 20.6 kDa
      AP_Mol_Mass 18-20 kDa

      Properties

      Purity > 95% as determined by reducing SDS-PAGE.
      Endotoxin < 1.0 EU per μg as determined by the LAL method.
      Storage Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
      Shipping This product is provided as lyophilized powder which is shipped with ice packs.
      Formulation Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
      Reconstitution Please refer to the printed manual for detailed information.

      Background

      Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.

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