Recombinant Thioredoxin-2/TXN2 Monoclonal Antibody (AN300424P)
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For research use only.
| Verified Samples |
Verified Samples in WB: K562, Mouse brain, Rat heart tissues in IHC: paraffin-embedded human colon cancer tissue in IF: HeLa |
| Dilution | WB 1:2000-1:20000, IHC-P 1:200-1:500, ICC/IF 1:100-1:200, FC 1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P, ICC/IF, FC |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human Thioredoxin-2/TXN2 protein |
| Abbre | TXN2 |
| Synonyms | TRX, TXN, Thioredoxin, MTRX, Mt-Trx, Thioredoxin Mitochondrial, Thioredoxin-2, TRX2, TXN2 |
| Swissprot | |
| Calculated MW | 18 kDa |
| Observed MW |
13 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Widely expressed in adult (at protein level) and fetal tissues. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Affinity Purification |
| Research Areas | Signal Transduction, Cancer, Metabolism |
| Clone | B350 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. [provided by RefSeq, Jul 2008] |
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