Recombinant Tie2/CD202b/TEK Monoclonal Antibody (AN300201P)

Name: Recombinant Tie2/CD202b/TEK Monoclonal Antibody
Brand: Elabscience
SKU: AN300201P
Price: 150.0 USD
Availability: InStock

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Recombinant Tie2/CD202b/TEK Monoclonal Antibody - 1

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	100μL	$ 380.00	- +
	20μL	$ 150.00	- +

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Dilution	WB: 1:1000；ICC/IF: 1:500-1:5000；FC: 1:200-1:500
Isotype	IgG
Host	Rabbit
Reactivity	Human, Mouse
Applications	WB, ICC/IF, FC
Clonality	Recombinant;Monoclonal
Immunogen	Recombinant Human Tie2 / CD202b / TEK protein
Abbre	TEK
Synonyms	TIE, TEK, CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1, Tie2 (Tek), Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202b antigen, endothelial, Endothelial tyrosine kinase, Endothelium specific receptor tyrosine kinase 2, hTIE 2, hTIE2, Hyk, p140 TEK, Soluble TIE2 variant 1, Soluble TIE2 variant 2, tek tyrosine kinase, TEK tyrosine kinase endothelial, TIE 2, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains 2, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine protein kinase receptor TEK, Tyrosine protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, Venous malformations multiple cutaneous and mucosal, VMCM 1
Swissprot	Q02763 Q02858
Calculated MW	126 kDa
Observed MW	160 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Cell membrane, Cell junction, Cell junction, focal adhesion Cytoplasm, cytoskeleton Secreted.
Concentration	1 mg/mL
Buffer	0.2 μm filtered solution in PBS
Purification Method	Protein A
Research Areas	Signal Transduction, Cancer, Cardiovascular, Stem Cells, Developmental Biology
Clone	B119
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]