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Recombinant Transferrin Receptor 1 Monoclonal Antibody

Cat:E-AB-81490
Manual MSDS

Price: $ 260

Price: $ 160

Size:
100μL 50μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human
  • Applications: WB;IF
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:K562,Hela
Verified Samples in IF: Hela
Dilution

WB 1:500-1:1000, IF 1:20-1:200

Western Blot Operation Guide
Clonality Rabbit Monoclonal
Immunogen Recombinant protein of human Transferrin Receptor
Abbre Transferrin Receptor 1
Synonyms TFRC;CD71;IMD46;T9;TFR;TFR1;TR;TRFR;p90
Swissprot
Calculated MW 84kDa
Observed MW 90kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Concentration 300 μg/mL
Buffer 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Purification Method Affinity Purified
Research Areas Cancer; Cardiovascular; Metabolism; Immunology; Stem Cells
Clone No. R08-6H2
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background CD71,also known as transferrin receptor protein 1 (TfR1),is a transmembrane glycoprotein composed of two disulfide-linked monomers,each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.