Recombinant Uteroglobin/SCGB1A1 Monoclonal Antibody (AN300504P)
For research use only.
| Verified Samples | Verified Samples in WB: A549 |
| Dilution | WB 1:1000-1:5000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Mouse Uteroglobin/SCGB1A1 protein |
| Abbre | SCGB1A1 |
| Synonyms | Secretoglobin family 1A member, SCGB1A, CC16, CCPBP, CCSP, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, Clara cells 10 kDa secretory protein, PCB-binding protein, PCB-BP, Secretoglobin family 1A member 1, UG, UGL, UP1, UP-1, Uteroglobin, Cc10, Ugb, Utg, Scgb1a1 |
| Swissprot | |
| Calculated MW | 10 kDa |
| Observed MW |
16 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways). |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Affinity Purification |
| Clone | B434 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the secretoglobin family of small secreted proteins. The encoded protein has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma. [provided by RefSeq, May 2010] |
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