For research use only.
| Product Introduction | RIPA Lysis Buffer is a traditional rapid cell tissue lysate used as the preferred lysate for protein extraction from tissues or cells in the Western Blot assay. |
| Applications | WB |
| Storage | Store at -20°C for 12 months. |
| Shipping | Biological ice pack at 4 ℃ |
| Cautions |
1. All steps to lyse samples should be performed on ice or at 4°C. 2. It is recommended to add 10 μL PMSF and 10 μL Na3VO4 to 1 mL RAPI Lysis before use. If RIPA Lysis is precipitated, please dissolve at room temperature or in a warm water bath. 3. It is normal for a clear gelatinous substance to appear in the lysate of RIPA lysate. This transparent gel is a complex containing genomic DNA. Repeatedly blowing the sample with a pipette about 50 times can effectively reduce the viscosity of the sample. After centrifugation, the supernatant can be taken for subsequent experiments. 4. The tissue or cell sample lysed by RIPA Lysis Buffer cannot be measured by the Bradford method because of the high concentration of detergent in the lysis. It is recommended to measure the protein concentration by the BCA method. 5. For your safety and health, please wear the lab coat and disposable gloves before the experiments. |
Other Clones
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor®405
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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