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RIPA Lysis Buffer (Strong)

    • RIPA Lysis Buffer-Elabscience
    • RIPA Lysis Buffer-Elabscience
    • RIPA Lysis Buffer-Elabscience

      Catalog number:E-BC-R327

      • 20 mL
      • 50 mL
      • 100 mL
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      RIPA Lysis Buffer is a traditional rapid cell tissue lysate used as the preferred lysate for protein extraction from tissues or cells in the Western Blot assay.




      20 mL

      50 mL

      100 mL



        RIPA Lysis Buffer(Strong)

      20 mL

      50 mL

      100 mL



        100 mM PMSF

      200 μL

      500 μL

      1 mL



        100 mM Na3VO4

      200 μL

      500 μL

      1 mL



      1 copy


      1.For Tissue Samples

      a. Take the samples, wash the tissue thoroughly with pre-cooled PBS (0.01M, pH7.4) to remove the surface blood and internal debris.

      b. Weigh and smash the tissue, add an appropriate ratio of RIPA Lysis Buffer (add 10 μL PMSF and 10 μL Na3VOto 1 mL RIPA Lysis) and homogenizely lyse the tissue.

      It is recommended to homogenize according to the ratio of tissue weight: RIPA volume = 3:10. For example,add 1mL RIPA Lysis Buffer to 0.3 g tissue sample, the specific volume can be adjusted according to experimental requirements.

      c. Shake and lyse on the ice for 30 min after homogenization. Blow the sample repeatedly with a Pipette gun for about 50 times (under ice water bath conditions) to Make sure the DNA strand is broken and reduce the viscosity of sample.
      d. Centrifuge at 12,000 rpm for 10 min at 4°C.

      e. Take the supernatant and measure the protein concentration.

      2.For Cell Sample

      a. Collect the cells, wash them thoroughly with pre-cooled PBS (0.01 M, pH7.4) to remove the medium off (it is generally recommended to wash 3 times).

      b. Add an appropriate ratio of RIPA Lysate Buffer (add 10 μL PMSF and 10 μL Na3VO4 to1 mL RIPA Lysis) and lyse on the ice for 30 min.

      It is recommended to add 0.1 mL of RIPA Lysis Buffer to each well of a 6-well plates (the protein content in different cells may vary, and the volume of the RIPA Lysis Buffer added can be appropriately adjusted).

      c. Blow the sample repeatedly with a Pipette gun for about 50 times (under ice water bath conditions) to Make sure the DNA strand is broken and reduce the viscosity of sample. 

      d. Centrifuge at 12,000 rpm for 10 min at 4°C.

      e. Take the supernatant and measure the protein concentration.

      RIPA Lysis Buffer components

      50 mM Tris (pH7.4), 150 mM NaCl, 1% Triton X-100, 1% C24H39O4Na, 1 mM EDTA, 0.1% SDS, 10 mM NaF, 1 mM Na3VO4, 1 mM PMSF.



      Store at -20°C for 12 months.


      1. For the protein extraction with RIPA Lysis Buffer, the whole process must be on the ice or at 4°C.

      2. It is recommended to add 10 μL PMSF and 10 μL Na3VOto RAPI Lysis before use. If RIPA Lysis is crystalline state,dissolve at room temperature or in a warm water bath.

      3. A cloud of transparent gels may appear in the RIPA Lysis product,  which is a normal phenomenon because the lysis product is a compound containing genomic DNA.Blow the sample repeatedly with a Pipette gun for about 50 times, It can effectively reduce the sample viscosity,Take the supernatant after centrifugation for subsequent testing.

      4.The protein sample lysed by RIPA cannot be measured by the Bradford method because of the high concentration of detergent in the lysis. It is recommended to measure the protein concentration by the BCA method.

      5. For your safety and health, please wear the lab coat and disposable gloves before the experiments.


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      19. LncRNA H19 Regulates Lipopolysaccharide (LPS)-Induced Apoptosis and Inflammation of BV2 Microglia Cells Through Targeting miR-325-3p/NEUROD4 Axis
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      20. MicroRNA-155 acts as a potential prognostic and diagnostic factor in patients with ankylosing spondylitis by modulating SOCS3
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        DOI: 10.1016/j.jff.2020.104209
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        Sample type: Liver Tissues
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        DOI: 10.1007/s11064-021-03279-0
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        Sample type: Cell Lysate(Primary Schwann Cells And Rsc96 Cells)
        Reactivity: Human
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        DOI: 10.1177/1753466620981858
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        Sample type: Tissue Slice( Lung Tissue)
        Reactivity: Rat
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        DOI: 10.1186/s12935-020-1097-2
        PMID: 31956297
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        Sample type: Cell Lysate
        Reactivity: Human
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        DOI: 10.1093/biolre/ioab227
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        DOI: 10.1111/jre.12973
        PMID: 35037251
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        Sample type: Periodontal Ligament Stem Cell (Pdlscs)
      53. Serum differential proteomic profiling of patients with isolated methylmalonic acidemia by iTRAQ
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        Journal: Frontiers in Genetics(2022)
        DOI: 10.3389/fgene.2022.765637
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      54. LINC02381 aggravates breast cancer through the miR-1271-5p/FN1 axis to activate PI3K/AKT pathway
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        DOI: 10.1002/mc.23375
        PMID: 34882856
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        Journal: JOURNAL OF NEUROCHEMISTRY(2019)
        DOI: 10.1111/jnc.14869
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      56. Placenta-specific 8 (PLAC8) mediates inflammation and mobility of the hPDLCs via MEK/ERK signaling pathway
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        DOI: 10.1016/j.intimp.2021.108459
        PMID: 34954560
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      57. Knockdown of circ-UQCRC2 ameliorated lipopolysaccharide-induced injury in MRC-5 cells by the miR-326/PDCD4/NF-κB pathway
        IF: 4.932
        DOI: 10.1016/j.intimp.2021.107633
        PMID: 33895481
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        Sample type: Cell Sample
        Reactivity: Human
      58. Effects of tocotrienol on osteocyte-mediated phosphate metabolism in high-carbohydrate high-fat diet-induced osteoporotic rats
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        DOI: 10.1016/j.jff.2022.105213
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        DOI: 10.3389/fendo.2022.871548
        PMID: 35634492
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      60. Silencing of OIP5-AS1 Protects Endothelial Cells From ox-LDL-Triggered Injury by Regulating KLF5 Expression via Sponging miR-135a-5p
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        DOI: 10.3389/fcvm.2021.596506
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      61. Enzalutamide-Induced Upregulation of PCAT6 Promotes Prostate Cancer Neuroendocrine Differentiation by Regulating miR-326/HNRNPA2B1 Axis
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        Journal: Frontiers in Oncology(2021)
        DOI: 10.3389/fonc.2021.650054
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      62. Anti-PADI4 antibody suppresses breast cancer by repressing the citrullinated fibronectin in the tumor microenvironment
        IF: 7.419
        DOI: 10.1016/j.biopha.2022.113289
        PMID: 35772376
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      63. SARS-CoV-2 nucleocapsid protein triggers hyperinflammation via protein-protein interaction-mediated intracellular Cl− accumulation in respiratory epithelium
        IF: 38.104
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        DOI: 10.1038/s41392-022-01048-1
        PMID: 35896532
        Products: E-BC-R327 
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