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RPL3 Polyclonal Antibody

Cat:E-AB-52628
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human; Mouse; Rat
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:RAW264.7,Raji,Jurkat
Dilution

WB 1:500-1:2000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Fusion protein of human RPL3
Abbre RPL3
Synonyms 60S ribosomal protein L3;HIV-1 TAR RNA-binding protein B;HIV1 TAR RNA binding protein B;MGC103388;MGC104284;MGC72934;Ribosomal protein L3;Ribosomal protein L3;isoform a;RL3;rpl3;TARBP B;TARBP-B
Swissprot
Calculated MW 46 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm.
Concentration 0.6 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Epigenetics and Nuclear Signaling; Immunology
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Ribosomes,the complexes that catalyze protein synthesis,consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3,also named as TARBP-B and MGC104284,belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA,and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins,there are multiple processed pseudogenes of this gene dispersed through the genome.