SDS-PAGE Gel kit

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      Catalog number:E-IR-R305

      Size:
      • 10-15T
      • 25-35T
      • 50-70T
      Qty:
      - +
      Price: $36

      Application: WB

      Storage: RT/2-8°C/-20°C

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Introduction

      Elabscience® SDS-PAGE Gel Assay kit is the classic SDS-PAGE gel preparation kit which contains all kinds of reagents needed to prepare the SDS-PAGE gel. Users only need apparatus and ddH2O to complete the gel preparation.

      The Gel Mix in this kit is the mixture of the gel buffer such as SDS and Tris-HCl and so on, which simplify the procedure of gel preparation. There are Tris-HCl pH 8.8 and SDS in the Separating Gel Mix, Tris-HCl pH 6.8 and SDS are in the Stacking Gel Mix.


      Compenonts



      Cat.

      Products

      10-15 T

      25-35 T

      50-70 T

      Storage

      E-IR-R305A

      30% Acr-Bis (29:1)

      45 mL

      100 mL

      100 mL x 2

      2~8°C

      E-IR-R305B

      Separating Gel Mix

      22 mL

      55 mL

      55 mL x 2

      RT

      E-IR-R305C

      Stacking Gel Mix

      6 mL

      12 mL

      22 mL

      RT

      E-IR-R305D

      10% APS

      1.5 mL

      2 x1.5 mL

      6 mL

      -20°C

      E-IR-R305E

      TEMED

      100 μL

      250 μL

      500 μL

      2~8°C

      Manual

       One Copy



      Instructions

      According to the different molecular weight of the target protein, different concentration of separating gel should be prepared.

      1. Comparison of protein separation linear range is shown in the manual.

      2. Sample volume of each component corresponding to different stacking gel volumes is shown in the manual.

      3. Sample volume of each component corresponding to different separation gel volumes is shown in the manual.


      Procedure

      1. Choose the clean gel mold and complete the assembly.

      2.  Refer to in the manual, prepare the separating gel of the required concentration and volume. Add appropriate amount of ddH2O, 30% Acr-Bis (29:1)[ E-IR-R305A] and Separating Gel Mix [E-IR-R305B] to a clean beaker, mix it.

      3.  Add appropriate amount of 10% APS and TEMED, mix gently and avoid bubbles.

      4.  Add the mixture gel to the assembled gel mold immediately. Then add 1-2 mL ddH2O or absolute alcohol on the separating gel to flatten the separating gel level.

      5.  Keep at RT for 30-60 min until the separating gel solidified completely.

      6.  Pour out the ddH2O or absolute ethanol, absorb the residual liquid with absorbent paper.

      7.  Refer to in the manual, prepare the stacking gel of the required volume. Add appropriate amount of ddH2O, 30% Acr-Bis (29:1)[ E-IR-R305A] and stacking Gel Mix [E-IR-R305C] to a clean beaker, mix it.

      8.  Add appropriate amount of 10% APS and TEMED, mix gently and avoid bubbles.

      9.  Add the mixture gel upon to the separating gel immediately. Insert comb teeth carefully, avoid bubbles.

      10. Keep at RT for 30-40 min until the stacking gel solidified completely.

      11. Pull out the comb teeth carefully. Follow the SDS-PAGE experiment.

       

      Storage

      Store separately.

      30% Acr-Bis (29:1) [E-IR-R305A] and TEMED[E-IR-R305E] should be stored at 2~8°C in dark.

      10% APS [E-IR-R305D] should be stored at -20°C.

      Other reagents should be stored at RT.


      Cautions

      1. For maximal assay performance, this reagent should be used within 6 months. Avoid freeze / thaw cycles.

      2. Please separate the 10% APS [E-IR-R305D] in different tubes and store at -20°C, this reagent shelf life is 6 months. If store at 4°C,please do not use it after one week.

      3. TEMED is volatile. Please tighten the cap after use. Due to the different temperatures, the rate of gel solidification will be different. The amount of TEMED can be adjusted appropriately to adjust the rate of gelation at different temperatures.

      4.  Avoid bubbles to avoid affecting the experimental results.

      5.  Add the ddH2O or absolute alcohol on the separating gel gently to avoid uneven line pressing.

      6.  This kit is for research use only. For your safety and health, please wear lab clothes and gloves. Instructions should be followed strictly, changes of operation may result in unreliable results. 

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