Sialic Acid (SA) Colorimetric Assay Kit (E-BC-K068-M)
For research use only.
| Detection Principle | Sialic acid forms a purplish red complex with methyl resorcinol in the presence of oxidant. And the absorbance conforms to Lambert-Beer's law. The content of sialic acid can be calculated by measuring the OD value at 560 nm. |
| Synonyms | SA |
| Sample Type | Serum,Plasma,Saliva,Urine,Hydrothorax,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (510-570 nm) |
| Research Area | Other |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.03 mmol/L |
| Detection Range | 0.03-7 mmol/L |
| Precision | inter-assay CV: 6.1% | intra-assay CV: 4.0% |
| Sample Volume | 25 μL |
| Assay Time | 40 min |
Note: Data for this module come from published literature that cites this product, displaying other products used together in the same research paper.
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Mouse serum | 1 |
| Porcine serum | 1 |
| Human plasma | 1 |
| Rat plasma | 1 |
| Mouse plasma | 1 |
| Human saliva | 1 |
| Human urine | 1 |
| Human hydrothorax | 1 |
| 10% Plant tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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