Sodium (Na) Colorimetric Assay Kit (E-BC-K207-M)
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For research use only.
| Detection Principle | β-galactosidase activated by sodium ions can catalyze glycoside analogues to produce glycoside and o-nitrophenol. The increase of absorbance is determined at 405 nm, and the content of sodium ion is calculated indirectly. |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (405 nm) |
| Research Area | Inorganic Ions , Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.02 mmol/L |
| Detection Range | 0.02-20 mmol/L |
| Precision | inter-assay CV: 8.4% | intra-assay CV: 2.2% |
| Sample Volume | 10 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| Rat plasma | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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