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SOX30 Polyclonal Antibody

Cat:E-AB-63588
Manual MSDS

Price: $ 530

Price: $ 320

Price: $ 200

Size:
200μL 120μL 60μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:U87-MG,U-251MG,Mouse testis,Mouse brain,Rat testis
Dilution

WB 1:500-1:2000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant fusion protein of human SOX30 (NP_848511.1).
Abbre SOX30
Synonyms SOX30
Swissprot
Calculated MW 54kDa/81kDa
Observed MW 90kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nuclear
Concentration 1mg/mL
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Affinity purification
Research Areas Epigenetics and Nuclear Signaling; Developmental Biology; Stem Cells
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein acts as a transcriptional regulator when present in a complex with other proteins. It can activate p53 transcription to promote tumor cell apoptosis in lung cancer. The protein may be involved in the differentiation of developing male germ cells. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 5.