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SP(Substance P) ELISA Kit

  • Cat.No.:E-EL-0067

  • Reactivity: Universal

To Purchase E-EL-0067

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 78.13-5000 pg/mL
Sensitivity 46.88 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes SP in samples. No significant cross-reactivity or interference between SP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of SP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SP. During the reaction, SP in the sample or standard competes with a fixed amount of SP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 233.17 510.94 2017.45 206.77 515.17 2371.94
Standard deviation 12.87 23.61 104.91 12.18 23.85 125.24
CV (%) 5.52 4.62 5.20 5.89 4.63 5.28


The recovery of SP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-96 93
EDTA plasma(n=8) 99-109 104
Cell culture media(n=8) 90-100 96

Target Information

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


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    Spinal cord astrocytes regulate myocardial ischemia–reperfusion injury

    DOI: 10.1007/s00395-022-00968-x

    Sample: serum
  2. Nutrients (2022) IF: 6.706
    Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by Ligilactobacillus salivarius Li01

    DOI: 10.3390/nu14194083

    PMID: 36235735

    Sample: serum
  3. PHYTOMEDICINE (2021) IF: 5.34
    Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa

    DOI: 10.1016/j.phymed.2021.153875

    PMID: 34911003

    Sample: serum
  4. Food & Function (2022) IF: 5.396
    A randomised, double-blind, placebo-controlled trial of Bifidobacterium bifidum CCFM16 for manipulation of gut microbiota and relief from chronic constipation

    DOI: 10.1039/D1FO03896F

    Sample: serum
    Protective effect of the combination of essential oil from patchouli and tangerine peel against gastric ulcer in rats

    DOI: 10.1016/j.jep.2021.114645

    PMID: 34530094

    Sample: serum
    Laxative effect and mechanism of Tiantian Capsule on loperamide-induced constipation in rats

    DOI: 10.1016/j.jep.2020.113411

    PMID: 32980482

    Sample: Serum
    Atractylodes oil alleviates diarrhea-predominant irritable bowel syndrome by regulating intestinal inflammation and intestinal barrier via SCF/c-kit and MLCK/MLC2 pathways

    DOI: 10.1016/j.jep.2021.113925

    PMID: 33592255

    Sample: Serum,Tissue homogenate(colon)
  8. Aging-US (2020) IF: 4.831
    NO up-regulates migraine-related CGRP via activation of an Akt/GSK-3β/NF-κB signaling cascade in trigeminal ganglion neurons

    DOI: 10.18632/aging.103031

    Sample: Primary TGNs
  9. Frontiers in Medicine (2020) IF: 3.9
    Acute Effect of Transcutaneous Electroacupuncture on Globus Pharyngeus: A Randomized, Single-Blind, Crossover Trial

    DOI: 10.3389/fmed.2020.00179

    PMID: 32528966

    Sample: Plasma
  10. NEUROSCIENCE LETTERS (2020) IF: 2.274
    FSC231 can alleviate paclitaxel-induced neuralgia by inhibiting PICK1 and affecting related factors

    DOI: 10.1016/j.neulet.2020.135471

    PMID: 33207243

    Sample: Tissue homogenate
  11. BRAIN RESEARCH (2019) IF: 2.929
    Cervical sympathectomy modulates the neurogenic inflammatory neuropeptides following experimental subarachnoid hemorrhage in rats

    DOI: 10.1016/j.brainres.2019.146366

    PMID: 31401069

    Sample: Plasma,Tissue homogenate
  12. ORL-Journal for Oto-Rhino-Laryngology Head and Neck Surgery (2021) IF: 1.538
    Clinical Efficacy and Possible Mechanism of Endoscopic Vidian Neurectomy for House Dust Mite-Sensitive Allergic Rhinitis

    DOI: 10.1159/000511711

    Sample: serum
  13. Evidence-based Complementary and Alternative Medicine (2020) IF: 1.813
    Laxative Effects of Yangyin Tongmi Capsule on a Model of Diphenoxylate-Induced Constipation in Mice

    DOI: 10.1155/2020/1471824

    PMID: 32148532

    Sample: Serum
  14. Revista da Associacao Medica Brasileira (2019) IF: 0.801
    Modulatory effects of neuropeptides on pentylenetetrazol-induced epileptic seizures and neuroinflammation in rats

    DOI: 10.1590/1806-9282.65.9.1188

    PMID: 31618336

    Sample: Plasma
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