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Cat.No.:E-EL-0067
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Assay type | Competitive-ELISA |
Format | 96T/48T |
Assay time | 2.0h |
Detection range | 78.13-5000 pg/mL |
Sensitivity | 46.88 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 50μL |
Specificity | This kit recognizes SP in samples. No significant cross-reactivity or interference between SP and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of SP concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SP were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 233.17 | 510.94 | 2017.45 | 206.77 | 515.17 | 2371.94 |
Standard deviation | 12.87 | 23.61 | 104.91 | 12.18 | 23.85 | 125.24 |
CV (%) | 5.52 | 4.62 | 5.20 | 5.89 | 4.63 | 5.28 |
The recovery of SP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 86-96 | 93 |
EDTA plasma(n=8) | 99-109 | 104 |
Cell culture media(n=8) | 90-100 | 96 |
Research Area | Neuroscience, Cardiovascular |
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1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
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2. Aspirate and wash the plate for 3 times |
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3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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5. Add 50μL Stop Solution |
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6. Read the plate at 450nm immediately. Calculation of the results |
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Sample: serum |
DOI: 10.3390/nu14194083 |
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PMID: 36235735 |
Sample: serum |
PMID: 34911003 |
Sample: serum |
DOI: 10.1039/D1FO03896F |
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Sample: serum |
PMID: 34530094 |
Sample: serum |
PMID: 32980482 |
Sample: Serum |
PMID: 33592255 |
Sample: Serum,Tissue homogenate(colon) |
Sample: Primary TGNs |
PMID: 32528966 |
Sample: Plasma |
PMID: 33207243 |
Sample: Tissue homogenate |
PMID: 31401069 |
Sample: Plasma,Tissue homogenate |
DOI: 10.1159/000511711 |
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Sample: serum |
DOI: 10.1155/2020/1471824 |
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PMID: 32148532 |
Sample: Serum |
PMID: 31618336 |
Sample: Plasma |