Current Position:Home>>Kits>>ELISA Kits>> ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit
Cat.No.:E-EL-0033
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Add to cart Bulk requestAssay type | Competitive-ELISA |
Format | 96T/48T |
Assay time | 2.0h |
Detection range | 15.63-1000 ng/mL |
Sensitivity | 9.38 ng/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 50μL |
Specificity | This kit recognizes ST/5-HT in samples. No significant cross-reactivity or interference between ST/5-HT and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of ST/5-HT concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ST/5-HT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ST/5-HT were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 51.20 | 141.30 | 362.70 | 54.70 | 132.00 | 351.00 |
Standard deviation | 3.50 | 5.90 | 15.60 | 3.10 | 6.50 | 16.10 |
CV (%) | 6.84 | 4.18 | 4.30 | 5.67 | 4.92 | 4.59 |
The recovery of ST/5-HT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 95-107 | 100 |
EDTA plasma(n=8) | 86-99 | 92 |
Cell culture media(n=8) | 91-105 | 98 |
Samples were spiked with high concentrations of ST/5-HT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | 5HT, Enteramine, Thrombocytin, Thrombotonin, 3-(β-Aminoethyl)-5-hydroxyindole |
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1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
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2. Aspirate and wash the plate for 3 times |
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3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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5. Add 50μL Stop Solution |
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6. Read the plate at 450nm immediately. Calculation of the results |
Cat.No. | Product Name | Specifications |
E-ELIR-001 | ELISA Plate Coating Buffer(1×) | 100mL, 1000mL |
E-ELIR-002 | ELISA Plate Coating Buffer(5×) | 100mL, 1000mL |
E-ELIR-003 | ELISA Plate Blocking Buffer | 100mL, 1000mL |
E-ELIR-004 | Wash Buffer for Sandwich-ELISA(25×) | 100mL, 1000mL |
E-ELIR-005 | Wash Buffer for Competitive-ELISA(25×) | 100mL, 1000mL |
E-ELIR-006 | Stop Solution | 100mL, 1000mL |
E-ELIR-007 | HRP-conjugate Stabilizer | 10mL, 100mL, 500mL |
E-ELIR-008 | HRP-conjugate Diluent | 100mL, 1000mL |
E-ELIR-009 | Biotinylated Antibody Stabilizer | 10mL, 100mL, 500mL |
E-ELIR-0010 | Biotinylated Antibody Diluent | 10mL, 100mL, 500mL |
E-ELIR-0011 | Sample Diluent | 10mL, 100mL, 500mL |
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PMID: 28315323 |
Sample: Cell culture supernatant,Tissue homogenate,Plasma |
PMID: 32710955 |
Sample: Plasma,Platelet |
PMID: 30880256 |
Sample: Tissue homogenate |
PMID: 30880256 |
Sample: Tissue homogenate |
PMID: 33014123 |
Sample: Whole Blood |
DOI: 10.1002/tox.22741 |
|
PMID: 30884102 |
Sample: Serum |
PMID: 26826326 |
Sample: Tissue homogenate |
PMID: 26409178 |
Sample: Plasma |
PMID: 27013094 |
Sample: Tissue homogenate |
PMID: 99999999 |
Sample: Tissue homogenate |
PMID: 28177659 |
Sample: Tissue homogenate |
DOI: 99999999 |
|
PMID: 99999999 |
Sample: Serum |
DOI: 99999999 |
|
PMID: 99999999 |
Sample: Tissue homogenate |
PMID: 99999999 |
Sample: Serum |
PMID: 99999999 |
Sample: Serum |
M***********aSubmitted [ Dec 03 2018 ]
Asked: Please there are two components in Serotonin kit : 1. Concentrated Biotinylated Detection Ab(100x) 120 μL, How can I dilute it with Biotinylated Detection Ab Diluent 14 ml ?, Or how much quality in ( ml ) can I add from Biotinylated Detection Ab Diluent to 120 μL of Concentrated Biotinylated Detection Ab(100x)? 2. Concentrated HRP Conjugate ( 100x ) 120 μL, How can I dilute it with HRP Conjugate Diluent 14 ml? Or how much quality in ( ml ) can I add from HRP Conjugate Diluent to 120 μL Concentrated HRP Conjugate ( 100x )? -- thanx--
Reply
adminSubmitted [ Dec 03 2018 ]
Answered: 1.Prepare the 1x Biotinylated Detection Ab working solution by add 9μL of Biotinylated Detection Ab Diluent to each microlitre of Concentrated Biotinylated Detection Ab(100x), the dilution ratio is 9:1. 2. Prepare the 1x HRP Conjugate working solution by add 9μL of HRP Conjugate Diluent to each microlitre of Concentrated HRP Conjugate( 100x ), the dilution ratio is 9:1.
D*****************ySubmitted [ Aug 14 2018 ]
Asked: I Have couple of questions for the 5HT ELISA kit (E-EL-0033) you have: 1. How reliable is the serotonin antibody in terms of cross reactivity with N-acetylserotonin, melatonin, tryptamine and other biological amines? 2. What is the sensitivity as compared to the kits from other popular commercial sources like Enzo, etc? Thanks, Surajit
Reply
adminSubmitted [ Aug 14 2018 ]
Answered: 1. Our lab applied 5-HT coupled with BSA as the antigen to generate this kit, the antibody is mouse monoclonal with high specificity, we have also validated this kit with melatonin and tryptamine, almost no cross reactivity for the two analogues was observed. 2. The detection rang is 15.63~1000ng/mL and the sensitivity is 9.38ng/mL for our kit.
e****aSubmitted [ Jul 28 2018 ]
Asked: may i have the question whether this elisa kit is suitable for mouse ?
Reply
adminSubmitted [ Jul 28 2018 ]
Answered: Dear eivira, Thanks for your question. This item with cat. #E-EL-0033 is an universal ELISA kit, and it's suitable for mouse. Please feel free to contact us if any question. Thank you.