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T(Testosterone) ELISA Kit

  • Cat.No.:E-EL-0155

  • Reactivity: Universal

To Purchase E-EL-0155

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 0.31-20 ng/mL
Sensitivity 0.17 ng/mL
Sample type &Sample volume serum, plasma, urine, saliva; 50μL
Specificity This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with T. During the reaction, T in the sample or standard competes with a fixed amount of T on the solid phase supporter for sites on the Biotinylated Detection Ab specific to T. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of T in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level T were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level T were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.33 5.22 10.88 1.54 5.73 10.02
Standard deviation 0.11 0.35 0.46 0.14 0.35 0.68
CV (%) 8.13 6.72 4.27 9.23 6.18 6.75


The recovery of T spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 80-90 84
EDTA plasma(n=8) 82-94 87
Urine(n=8) 85-102 95


Samples were spiked with high concentrations of T and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal Of Hazardous Materials (2020) IF: 9.038
    Multiple transcriptomic profiling: p53 signaling pathway is involved in DEHP-induced prepubertal testicular injury via promoting cell apoptosis and inhibiting cell proliferation of Leydig cells

    DOI: 10.1016/j.jhazmat.2020.124316

    PMID: 33162236

    Sample: Serum
  2. Particle and Fibre Toxicology (2022) IF: 9.4
    Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway

    DOI: 10.1186/s12989-022-00453-2

    PMID: 35177090

    Sample: Serum,Cell culture supernatant
  3. Environmental Pollution (2020) IF: 6.792
    Environmental relevant concentration of λ-cyhalothrin and 3-phenoxybenzoic acid caused endocrine-disrupting effects on male lizards (Eremias argus)

    DOI: 10.1016/j.envpol.2020.115077

    Sample: Plasma
  4. Diabetologia (2018) IF: 6.023
    Prostaglandin E2/EP2 receptor signalling pathway promotes diabetic retinopathy in a rat model of diabetes.

    DOI: 10.1007/s00125-018-4755-3

    PMID: 30411254

    Sample: Cell culture supernatant
  5. Food&Function (2022) IF: 5.396
    Lactiplantibacillus plantarum CCFM1019 attenuate polycystic ovary syndrome through butyrate dependent gut–brain mechanism

    DOI: 10.1039/d1fo01744f

    PMID: 35044398

    Sample: Serum
  6. Cell Death & Disease (2017) IF: 5.965
    ANP Promotes Proliferation And Inhibits Apoptosis Of Ovarian Granulosa Cells By NPRA/PGRMC1/EGFR Complex And Improves Ovary Functions Of PCOS Rats.

    DOI: 10.1038/cddis.2017.494

    PMID: 29072679

    Sample: Serum
  7. Cellular Physiology and Biochemistry (2017) IF: 5.104
    Protective Effects Of Fluvastatin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet Through Enhanced Signaling Of mTOR.

    DOI: 10.1159/000457881

    PMID: 28214901

    Sample: Serum
  8. Toxicology (2021) IF: 4.221
    Environmentally relevant perinatal exposure to DBP disturbs testicular development and puberty onset in male mice

    DOI: 10.1016/j.tox.2021.152860

    PMID: 34280466

    Sample: Serum
  9. Food & Function (2020) IF: 4.171
    Lactic acid bacteria alleviate polycystic ovarian syndrome by regulating sex hormone related gut microbiota

    DOI: 10.1039/c9fo02554e

    PMID: 32441726

    Sample: Serum
  10. journal of molecular sciences (2019) IF: 4.183
    Sulforaphane Protect Against Cadmium-Induced Oxidative Damage in mouse Leydigs Cells by Activating Nrf2/ARE Signaling Pathway

    DOI: 10.3390/ijms20030630

    PMID: 30717178

    Sample: Tissue homogenate
  11. Science of The Total Environment (2019) IF: 4.61
    Accumulation and toxicity of thiamethoxam and its metabolite clothianidin to the gonads of Eremias argus

    DOI: 10.1016/j.scitotenv.2019.02.419

    PMID: 30833257

    Sample: Plasma
  12. Clinical Science (2018) IF: 4.936
    CTRP3 attenuates high-fat diet-induced male reproductive dysfunction in mice.

    DOI: 10.1042/CS20180179

    PMID: 29572383

  13. Scientific Reports (2017) IF: 4.259
    Diet-Induced Obesity Impairs Spermatogenesis: A Potential Role For Autophagy.

    DOI: 10.1038/srep43475

    PMID: 28276438

    Sample: Serum
  14. Journal of Ginseng Research (2016) IF: 4.082
    InVitro And InVivo Evaluation Of Tissue-Cultured Mountain Ginseng OnPenile Erection.

    DOI: 10.1016/j.jgr.2015.10.003

    PMID: 27746685

    Sample: Serum
  15. Chemosphere (2017) IF: 4.208
    Disruption Of Sex-Hormone Levels And Steroidogenic-Related Gene Expression On Mongolia Racerunner (Eremias Argus) After Exposure To Triadimefon And Its Enantiomers.

    DOI: 10.1016/j.chemosphere.2016.12.096

    PMID: 28039834

    Sample: Plasma
  16. Nitric Oxide (2020) IF: 3.311
    Estrogen receptor (ESR1 and ESR2)-mediated activation of eNOS-NO-cGMP pathway facilitates high altitude acclimatization

    DOI: 10.1016/j.niox.2020.05.003

    PMID: 32544536

    Sample: Plasma
  17. Life Sciences (2020) IF: 3.647
    Protective role of glutamine against cadmium-induced testicular dysfunction in Wistar rats: Involvement of G6PD activity

    DOI: 10.1016/j.lfs.2019.117250

    PMID: 31899225

    Sample: Serum
  18. Ecotoxicology and environmental safety (2018) IF: 3.974
    Sulphur dioxide and arsenic affect male reproduction via interfering with spermatogenesis in mice

    DOI: 10.1016/j.ecoenv.2018.08.109

    PMID: 30195209

    Sample: Serum
  19. Toxicology and applied pharmacology (2019) IF: 3.616
    High copper levels in follicular fluid affect follicle development in polycystic ovary syndrome patients: Population-based and in vitro studies

    DOI: 10.1016/j.taap.2019.01.008

    PMID: 30641075

    Sample: Serum,Cell culture medium
  20. Ecotoxicology and environmental safety (2019) IF: 3.974
    Ecological risk assessment of alpha-cypermethrin-treated food ingestion and reproductive toxicity in reptiles

    DOI: 10.1016/j.ecoenv.2019.01.012

    PMID: 30658301

    Sample: Serum
  21. Toxins (2018) IF: 3.273
    The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice

    DOI: 10.3390/toxins10110449

    PMID: 30384460

    Sample: Serum
  22. Scientific Reports (2020) IF: 3.998
    Reproductive effects of subchronic exposure to acetamiprid in male rats

    DOI: 10.1038/s41598-020-65887-0

    PMID: 32488017

    Sample: Plasma,Tissue homogenate
  23. Journal of Hazardous Materials (2020) IF: 9.038
    Jinxin oral liquid inhibits human respiratory syncytial virus-induced excessive inflammation associated with blockade of the NLRP3/ASC/Caspase-1 pathway.

    DOI: 10.1016/j.jhazmat.2020.123430

    PMID: 32659591

    Sample: Serum
  24. Toxicology Letters (2017) IF: 3.858
    Dual Effects Of Sulfasalazine On Rat Sperm Characteristics, Spermatogenesis, And Steroidogenesis In Two Experimental Models.

    DOI: 10.1016/j.toxlet.2017.11.034

    PMID: 29197623

    Sample: Serum
  25. Toxicology Letters (2014) IF: 3.858
    Differential Proteome And Gene Expression Reveal Response To Carbon Ion Irradiation In Pubertal Mice Testes.

    DOI: 10.1016/j.toxlet.2014.01.001

    PMID: 24440814

    Sample: Serum
  26. Reproductive Sciences (2020) IF: 2.616
    Ascorbic Acid and Alpha-Tocopherol Contribute to the Therapy of Polycystic Ovarian Syndrome in Mouse Models

    DOI: 10.1007/s43032-020-00273-9

    PMID: 32725591

    Sample: Serum
  27. Biology of Reproduction (2020) IF: 2.96
    Loss of Fbxw7 in Sertoli cells impairs testis development and causes infertility in mice

    DOI: 10.1016/j.jtemb.2020.126544

    Sample: Serum
  28. International journal of molecular medicine (2019) IF: 2.784
    Mitochondria-targeted antioxidant therapy for an animal model of PCOS-IR

    DOI: 10.3892/ijmm.2018.3977

    PMID: 30431108

    Sample: Serum
  29. Journal of Applied Toxicology (2018) IF: 2.909
    Maternal di-(2-ethylhexyl) phthalate exposure alters hepatic insulin signal transduction and glucoregulatory events in rat F1 male offspring

    DOI: 10.1002/jat.3764

    PMID: 30565266

    Sample: Serum
  30. Environmental Science and Pollution Research (2019) IF: 2.8
    Isolation, characterization, and therapeutic activity of bergenin from marlberry (Ardisia colorata Roxb.) leaf on diabetic testicular complications in Wistar albino rats

    DOI: 10.1007/s11356-019-04139-9

    PMID: 30648235

    Sample: Serum
  31. International Journal of Molecular Medicine (2018) IF: 2.784
    Expression of FKBP52 in the ovaries of PCOS rats

    DOI: 10.3892/ijmm.2018.3998

    PMID: 30483787

    Sample: Serum
  32. Environmental toxicology (2018) IF: 2.776
    Di-(2-ethylhexyl) phthalate (DEHP)-induced testicular toxicity through Nrf2-mediated Notch1 signaling pathway in Sprague-Dawley rats.

    DOI: 10.1002/tox.22559

    PMID: 29663635

    Sample: Tissue homogenate
  33. Autonomic Neuroscience-Basic & Clinical (2015) IF: 2.225
    Brain Thromboxane A2 Via Arachidonic Acid Cascade Induces The Hypothalamic-Pituitary-Gonadal Axis Activation In Rats.

    DOI: 10.1016/j.autneu.2015.02.005

    PMID: 25784152

    Sample: Plasma
  34. Plos One (2017) IF: 2.806
    Insulin Resistance Enhances The Mitogen-Activated Protein Kinase Signaling Pathway In Ovarian Granulosa Cells.

    DOI: 10.1371/journal.pone.0188029

    PMID: 29125859

    Sample: Cell culture supernatant
  35. Journal of Assisted Reproduction & Genetics (2015) IF: 2.163
    Protective Effects Of Metformin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet.

    DOI: 10.1007/s10815-015-0506-2

    PMID: 26081124

    Sample: Serum
  36. Journal of Food Biochemistry (2020) IF: 1.662
    Berberine mitigates oxidative damage associated with testicular impairment following mercury chloride intoxication

    DOI: 10.1111/jfbc.13385

    Sample: Tissue homogenate
  37. Journal of Applied Biomedicine (2018) IF: 1.783
    Pre-pregnancy stress suppressed the reproductive systems in parents and changed sex ratio in offspring

    DOI: 10.1016/j.jab.2018.08.002

    Sample: Plasma
  38. Experimental and Therapeutic Medicine IF: 1.41
    Analysis of gene expression and functional changes of adrenal gland in a rat model of kidney yang deficiency syndrome treated with Sini decoction.

    DOI: 10.3892/etm.2018.6521

    PMID: 30214533

    Sample: Serum
  39. Molecular Medicine Reports (2016) IF: 1.692
    Curcumin Ameliorates HighFat DietInduced Spermatogenesis Dysfunction.

    DOI: 10.3892/mmr.2016.5712

    PMID: 27600729

    Sample: Serum
  40. Molecular Medicine Reports (2016) IF: 1.692
    Curcumin Ameliorates High-Fat Diet-Induced Spermatogenesis Dysfunction.

    DOI: 10.3892/mmr.2016.5712

    PMID: 27600729

    Sample: Serum
  41. Journal of Hazardous Materials (2020) IF: 9.038
    Polystyrene microplastics induced male reproductive toxicity in mice

    DOI: 10.1016/j.jhazmat.2020.123430

    PMID: 32659591

    Sample: Serum
  42. Indian J Physiol Pharmacol (2018)
    Short Communication Combined Effects of Vitamin C and Tomato Extract (Lycopersicon Esculentum) on Carbimazole-induced Alterations in the Testes of Male Albino Rats
    Sample: Serum
  43. International Journal of Complementary & Alternative Medicine (2018)
    The role of red betel extracts (piper crocatum ruiz & pav.) against testicular assessment on mice model of rheumatoid arthritis
    Sample: Serum
  44. Indian J Physiol Pharmacol (2018) IF: /
    Short Communication Combined Effects of Vitamin C and Tomato Extract (Lycopersicon Esculentum) on Carbimazole-induced Alterations in the Testes of Male Albino Rats.
    Sample: Serum
  45. Journal of Advances in Medicine and Medical Research (2018) IF: /
    Adequate Exercise Improves Inflammatory, Gonadal Markers and Sexual Function in Type 2 Diabetics.

    DOI: 10.9734/JAMMR/2018/39766

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  • Q&A

G*****dSubmitted [ May 03 2022 ]

Asked: Hello, In the ELISA kit E-EL-0155, did we measure free or total testosterone ? Best regards, Geraldine



adminSubmitted [ May 03 2022 ]

Answered: Hi Geraldine, this kit measures Total Testosterone. Feel free to contact us if you have any other questions.

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