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T(Testosterone) ELISA Kit

  • Cat.No.:E-EL-0155

  • Reactivity: Universal

To Purchase E-EL-0155

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 0.31-20 ng/mL
Sensitivity 0.17 ng/mL
Sample type &Sample volume serum, plasma, urine, saliva; 50μL
Specificity This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with T. During the reaction, T in the sample or standard competes with a fixed amount of T on the solid phase supporter for sites on the Biotinylated Detection Ab specific to T. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of T in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level T were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level T were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.33 5.22 10.88 1.54 5.73 10.02
Standard deviation 0.11 0.35 0.46 0.14 0.35 0.68
CV (%) 8.13 6.72 4.27 9.23 6.18 6.75


The recovery of T spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 80-90 84
EDTA plasma(n=8) 82-94 87
Urine(n=8) 95


Samples were spiked with high concentrations of T and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information


Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


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View more details about our Publication Reward >>

  1. Environmental Pollution (2020) IF: 6.792
    Environmental relevant concentration of λ-cyhalothrin and 3-phenoxybenzoic acid caused endocrine-disrupting effects on male lizards (Eremias argus)

    DOI: 10.1016/j.envpol.2020.115077

    PMID: 99999999

    Sample: Plasma
  2. Diabetologia (2018) IF: 6.023
    Prostaglandin E2/EP2 receptor signalling pathway promotes diabetic retinopathy in a rat model of diabetes.

    DOI: 10.1007/s00125-018-4755-3

    PMID: 30411254

    Sample: Cell culture supernatant
  3. Cell Death & Disease (2017) IF: 5.965
    ANP Promotes Proliferation And Inhibits Apoptosis Of Ovarian Granulosa Cells By NPRA/PGRMC1/EGFR Complex And Improves Ovary Functions Of PCOS Rats.

    DOI: 10.1038/cddis.2017.494

    PMID: 29072679

    Sample: Serum
  4. Cellular Physiology and Biochemistry (2017) IF: 5.104
    Protective Effects Of Fluvastatin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet Through Enhanced Signaling Of mTOR.

    DOI: 10.1159/000457881

    PMID: 28214901

    Sample: Serum
  5. Food & Function (2020) IF: 4.171
    Lactic acid bacteria alleviate polycystic ovarian syndrome by regulating sex hormone related gut microbiota

    DOI: 10.1039/c9fo02554e

    PMID: 32441726

    Sample: Serum
  6. journal of molecular sciences (2019) IF: 4.183
    Sulforaphane Protect Against Cadmium-Induced Oxidative Damage in mouse Leydigs Cells by Activating Nrf2/ARE Signaling Pathway

    DOI: 10.3390/ijms20030630

    PMID: 30717178

    Sample: Tissue homogenate
  7. Science of The Total Environment (2019) IF: 4.61
    Accumulation and toxicity of thiamethoxam and its metabolite clothianidin to the gonads of Eremias argus

    DOI: 10.1016/j.scitotenv.2019.02.419

    PMID: 30833257

    Sample: Plasma
  8. Clinical Science (2018) IF: 4.936
    CTRP3 attenuates high-fat diet-induced male reproductive dysfunction in mice.

    DOI: 10.1042/CS20180179

    PMID: 29572383

  9. Scientific Reports (2017) IF: 4.259
    Diet-Induced Obesity Impairs Spermatogenesis: A Potential Role For Autophagy.

    DOI: 10.1038/srep43475

    PMID: 28276438

    Sample: Serum
  10. Journal of Ginseng Research (2016) IF: 4.082
    InVitro And InVivo Evaluation Of Tissue-Cultured Mountain Ginseng OnPenile Erection.

    DOI: 10.1016/j.jgr.2015.10.003

    PMID: 27746685

    Sample: Serum
  11. Chemosphere (2017) IF: 4.208
    Disruption Of Sex-Hormone Levels And Steroidogenic-Related Gene Expression On Mongolia Racerunner (Eremias Argus) After Exposure To Triadimefon And Its Enantiomers.

    DOI: 10.1016/j.chemosphere.2016.12.096

    PMID: 28039834

    Sample: Plasma
  12. Nitric Oxide (2020) IF: 3.311
    Estrogen receptor (ESR1 and ESR2)-mediated activation of eNOS-NO-cGMP pathway facilitates high altitude acclimatization

    DOI: 10.1016/j.niox.2020.05.003

    PMID: 32544536

    Sample: Plasma
  13. Life Sciences (2020) IF: 3.647
    Protective role of glutamine against cadmium-induced testicular dysfunction in Wistar rats: Involvement of G6PD activity

    DOI: 10.1016/j.lfs.2019.117250

    PMID: 31899225

    Sample: Serum
  14. Ecotoxicology and environmental safety (2018) IF: 3.974
    Sulphur dioxide and arsenic affect male reproduction via interfering with spermatogenesis in mice

    DOI: 10.1016/j.ecoenv.2018.08.109

    PMID: 30195209

    Sample: Serum
  15. Toxicology and applied pharmacology (2019) IF: 3.616
    High copper levels in follicular fluid affect follicle development in polycystic ovary syndrome patients: Population-based and in vitro studies

    DOI: 10.1016/j.taap.2019.01.008

    PMID: 30641075

    Sample: Serum,Cell culture medium
  16. Ecotoxicology and environmental safety (2019) IF: 3.974
    Ecological risk assessment of alpha-cypermethrin-treated food ingestion and reproductive toxicity in reptiles

    DOI: 10.1016/j.ecoenv.2019.01.012

    PMID: 30658301

    Sample: Serum
  17. Toxins (2018) IF: 3.273
    The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice

    DOI: 10.3390/toxins10110449

    PMID: 30384460

    Sample: Serum
  18. Scientific Reports (2020) IF: 3.998
    Reproductive effects of subchronic exposure to acetamiprid in male rats

    DOI: 10.1038/s41598-020-65887-0

    PMID: 32488017

    Sample: Plasma,Tissue homogenate
  19. Journal of Hazardous Materials (2020) IF: 9.038
    Jinxin oral liquid inhibits human respiratory syncytial virus-induced excessive inflammation associated with blockade of the NLRP3/ASC/Caspase-1 pathway.

    DOI: 10.1016/j.jhazmat.2020.123430

    PMID: 32659591

    Sample: Serum
  20. Toxicology Letters (2017) IF: 3.858
    Dual Effects Of Sulfasalazine On Rat Sperm Characteristics, Spermatogenesis, And Steroidogenesis In Two Experimental Models.

    DOI: 10.1016/j.toxlet.2017.11.034

    PMID: 29197623

    Sample: Serum
  21. Toxicology Letters (2014) IF: 3.858
    Differential Proteome And Gene Expression Reveal Response To Carbon Ion Irradiation In Pubertal Mice Testes.

    DOI: 10.1016/j.toxlet.2014.01.001

    PMID: 24440814

    Sample: Serum
  22. Reproductive Sciences (2020) IF: 2.616
    Ascorbic Acid and Alpha-Tocopherol Contribute to the Therapy of Polycystic Ovarian Syndrome in Mouse Models

    DOI: 10.1007/s43032-020-00273-9

    PMID: 32725591

    Sample: Serum
  23. Biology of Reproduction (2020) IF: 2.96
    Loss of Fbxw7 in Sertoli cells impairs testis development and causes infertility in mice

    DOI: 10.1016/j.jtemb.2020.126544

    PMID: 99999999

    Sample: Serum
  24. International journal of molecular medicine (2019) IF: 2.784
    Mitochondria-targeted antioxidant therapy for an animal model of PCOS-IR

    DOI: 10.3892/ijmm.2018.3977

    PMID: 30431108

    Sample: Serum
  25. Journal of Applied Toxicology (2018) IF: 2.909
    Maternal di-(2-ethylhexyl) phthalate exposure alters hepatic insulin signal transduction and glucoregulatory events in rat F1 male offspring

    DOI: 10.1002/jat.3764

    PMID: 30565266

    Sample: Serum
  26. Environmental Science and Pollution Research (2019) IF: 2.8
    Isolation, characterization, and therapeutic activity of bergenin from marlberry (Ardisia colorata Roxb.) leaf on diabetic testicular complications in Wistar albino rats

    DOI: 10.1007/s11356-019-04139-9

    PMID: 30648235

    Sample: Serum
  27. International Journal of Molecular Medicine (2018) IF: 2.784
    Expression of FKBP52 in the ovaries of PCOS rats

    DOI: 10.3892/ijmm.2018.3998

    PMID: 30483787

    Sample: Serum
  28. Environmental toxicology (2018) IF: 2.776
    Di-(2-ethylhexyl) phthalate (DEHP)-induced testicular toxicity through Nrf2-mediated Notch1 signaling pathway in Sprague-Dawley rats.

    DOI: 10.1002/tox.22559

    PMID: 29663635

    Sample: Tissue homogenate
  29. Autonomic Neuroscience-Basic & Clinical (2015) IF: 2.225
    Brain Thromboxane A2 Via Arachidonic Acid Cascade Induces The Hypothalamic-Pituitary-Gonadal Axis Activation In Rats.

    DOI: 10.1016/j.autneu.2015.02.005

    PMID: 25784152

    Sample: Plasma
  30. Plos One (2017) IF: 2.806
    Insulin Resistance Enhances The Mitogen-Activated Protein Kinase Signaling Pathway In Ovarian Granulosa Cells.

    DOI: 10.1371/journal.pone.0188029

    PMID: 29125859

    Sample: Cell culture supernatant
  31. Journal of Assisted Reproduction & Genetics (2015) IF: 2.163
    Protective Effects Of Metformin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet.

    DOI: 10.1007/s10815-015-0506-2

    PMID: 26081124

    Sample: Serum
  32. Journal of Food Biochemistry (2020) IF: 1.662
    Berberine mitigates oxidative damage associated with testicular impairment following mercury chloride intoxication

    DOI: 10.1111/jfbc.13385

    PMID: 99999999

    Sample: Tissue homogenate
  33. Journal of Applied Biomedicine (2018) IF: 1.783
    Pre-pregnancy stress suppressed the reproductive systems in parents and changed sex ratio in offspring

    DOI: 10.1016/j.jab.2018.08.002

    PMID: 99999999

    Sample: Plasma
  34. Experimental and Therapeutic Medicine IF: 1.41
    Analysis of gene expression and functional changes of adrenal gland in a rat model of kidney yang deficiency syndrome treated with Sini decoction.

    DOI: 10.3892/etm.2018.6521

    PMID: 30214533

    Sample: Serum
  35. Molecular Medicine Reports (2016) IF: 1.692
    Curcumin Ameliorates HighFat DietInduced Spermatogenesis Dysfunction.

    DOI: 10.3892/mmr.2016.5712

    PMID: 27600729

    Sample: Serum
  36. Molecular Medicine Reports (2016) IF: 1.692
    Curcumin Ameliorates High-Fat Diet-Induced Spermatogenesis Dysfunction.

    DOI: 10.3892/mmr.2016.5712

    PMID: 27600729

    Sample: Serum
  37. Journal of Hazardous Materials (2020) IF: 9.038
    Polystyrene microplastics induced male reproductive toxicity in mice

    DOI: 10.1016/j.jhazmat.2020.123430

    PMID: 32659591

    Sample: Serum
  38. Indian J Physiol Pharmacol (2018)
    Short Communication Combined Effects of Vitamin C and Tomato Extract (Lycopersicon Esculentum) on Carbimazole-induced Alterations in the Testes of Male Albino Rats

    PMID: 99999999

    Sample: Serum
  39. International Journal of Complementary & Alternative Medicine (2018)
    The role of red betel extracts (piper crocatum ruiz & pav.) against testicular assessment on mice model of rheumatoid arthritis

    PMID: 99999999

    Sample: Serum
  40. Indian J Physiol Pharmacol (2018) IF: /
    Short Communication Combined Effects of Vitamin C and Tomato Extract (Lycopersicon Esculentum) on Carbimazole-induced Alterations in the Testes of Male Albino Rats.
    Sample: Serum
  41. Journal of Advances in Medicine and Medical Research (2018) IF: /
    Adequate Exercise Improves Inflammatory, Gonadal Markers and Sexual Function in Type 2 Diabetics.

    DOI: 10.9734/JAMMR/2018/39766

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