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Tau Monoclonal Antibody

Cat:E-AB-22141
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Mouse
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p
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Product Details
Verified Samples Verified Samples in WB:K562,Mouse brain,Rat brain
Verified Samples in IHC:Rat brain
Dilution

WB 1:1000-2000, IHC 1:100-200

Western Blot Operation Guide
Clonality Monoclonal
Immunogen Synthetic Peptide of Tau
Abbre Tau Mouse (10E3)
Synonyms AI413597;AW045860;DDPAC;FLJ31424;FTDP 17;G protein beta1/gamma2 subunit interacting factor 1;MAPT;MAPTL;MGC134287;MGC138549;MGC156663;Microtubule associated protein tau;Microtubule associated protein tau isoform 4;Microtubule-associated protein tau;MSTD;Mtapt;MTBT1;MTBT2;Neurofibrillary tangle protein;Paired helical filament tau;Paired helical filament-tau;PHF tau;PHF-tau;PPND;PPP1R103;Protein phosphatase 1;regulatory subunit 103;pTau;RNPTAU;TAU;TAU;Tauopathy and respiratory failure;included
Swissprot
Observed MW 79kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm>cytosol. Cell membrane. Cytoplasm>cytoskeleton. Cell projection>axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
Tissue Specificity Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Protein A purification
Research Areas Cancer; Neuroscience; Signal Transduction
Clone No. Clone:8A3
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.