TBST Buffer (10 ×)

Introduction

TBST buffer is used in Western Blot to wash away non-specific binding on the membrane, this buffer contains 0.1% Tween20. Tween20 is a non-ionic detergent that increases the buffer's ability to elute and reduce non-specific binding of antibodies. This product is supplied with 10 × concentrate and easy for transportation.

Components

Instructions

This product is a 10 × concentrate solution, dilute with pure water to 1  working solution before use.

For example: take 100 mL TBST Buffer (10 ×), dilute with pure water to 1 L.

1 × TBST Buffer Components

145.4 mM NaCl, 10 mM Tirs-base, 0.1% (v/v) Tween20, pH7.5.

Storage

Store at room temperature for 12 months.

Cautions

1.If the product crystallizes, please use water bath to dissolve at 37°C, and then dilute it after it is completely dissolved.

2.Keep the product sealed to prevent from pollution.

3.For your safety and health, please wear the lab coat and disposable gloves before the experiments.

Citations