TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit

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    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-0162

      Synonyms:Cartilage-inducing factor,CED,Differentiation inhibiting factor,DPD1,LAP,Latency-associated peptide,Prepro transforming growth factor beta 1,TGF beta 1,TGF beta,TGF beta 1 protein,TGF-beta 1 protein,TGF-beta-1,TGF-beta-5,TGF-beta1,TGFB,Tgfb-1,tgfb1,TGFB1,TGFbeta,TGFbeta1,Transforming Growth Factor b1,Transforming Growth Factor beta 1,Transforming growth factor beta 1a,transforming growth factor beta-1,transforming growth factor,beta 1,Transforming Growth Factor-ß1

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Universal

      Detection Range: 0.16~10 ng/mL

      Sensitivity: 0.10 ng/mL

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Intended use

      This ELISA kit applies to the in vitro quantitative determination of TGF-β1 concentrations in serum, plasma and other biological fluids.


      Introduction

      Transforming Growth Factor Beta-1 (TGF-β1) is a pleiotropic cytokine with potent anti-inflammatory property, and has been considered an essential risk factor in the inflammatory process of Ischemic Stroke (IS) by involving in the pathophysiological progression of lipid metabolisms, hypertension, and atherosclerosis. TGF-β1 gene is located on chromosome 19 (q13.1–13.3), including 7 exons and 6 introns. The TGFβ-1 protein is found throughout the body but is particularly abundant in tissues that make up the skeleton, where it helps regulate the formation and growth of bone and cartilage, a tough, flexible tissue that makes up much of the skeleton during early development. TGFβ-1 is also involved in the formation of blood vessels, development of muscle tissue and body fat, wound healing, inflammatory processes in the immune system, and prevention of tumor growth.


      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro-plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of TGF-β1. You can calculate the concentration of TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

      Assay type

      Sandwich-ELISA

      Format

      96T

      Assay time

      3.5h

      Reactivity

      Universal

      Detection Method

      Colormetric

      Detection Range

      0.16—10 ng/mL

      Sensitivity

      0.10 ng/mL

      Sample Volume Required Per Well

      100μL

      Sample Type

      Serum, plasma and other biological fluids

      Specificity

      This kit recognizes TGF-β1 in samples. No significant cross-reactivity or interference between TGF-β1 and analogues was observed

      Repeatability

      Coefficient of variation is <10%


      Sample Activation

      TGF-β1 in biological samples usually exist in unactivated forms. So it must be activated before testing. Follow the activation procedure outlined below:

      Serum/ Plasma: Add 40uL samples into 280uL Reference Standard & Sample Diluent, mix well, then add 40uL of Activator reagent 1, incubate 10 minutes at room temperature. Neutralize the acidified sample by adding 40 μL of Activator reagent 2, mix well and test immediately.

      Note: Sample is diluted 10 times.

      Cell Culture Supernatant: Add 100uL samples into 20uL Reference Standard & Sample Diluent, mix well, then add 40uL of Activator reagent 1, incubate 10 minutes at room temperature. Neutralize the acidified sample by adding 40 μL of Activator reagent 2, mix well and test immediately.

      Note: Sample is diluted 2 times.

      Tissue homogenates: Add 40uL samples into 1960uL Reference Standard & Sample Diluent, mix well and test.

      Note: Sample is diluted 50 times.


      Sample value

      Serum/Plasma –Samples from apparently healthy human, mouse, rat, porcine, rabbit, canine were evaluated for detectable levels of TGF-β1 in this assay.

      Sample Type

      Source

      Range

      Dilution Factor

      Serum

      Healthy Human

      22.8-46.1 ng/mL

      10

      Plasma

      Healthy Human

      12.7-43.1 ng/mL

      10

      Serum

      Healthy Mouse

      66.1-82.9 ng/mL

      10

      Serum

      Healthy Rat

      35.4-66.5 ng/mL

      10

      Serum

      Healthy Porcine

      4.9-10.2 ng/mL

      10

      Serum

      Healthy Rabbit

      2.9-6.5 ng/mL

      10

      Serum

      Healthy Canine

      34.5-84.2 ng/mL

      10


      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration(ng/mL)

      10

      5

      2.5

      1.25

      0.63

      0.32

      0.16

      0

      OD

      2.515

      1.645

      0.988

      0.506

      0.271

      0.183

      0.111

      0.057

      Corrected OD

      2.458

      1.588

      0.931

      0.449

      0.214

      0.126

      0.054

      -



      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level TGF-β1 were tested 20 times on one plate, respectively.

      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level TGF-β1 were tested on 3 different plates, 20 replicates in each plate.

       

      Intra-assay Precision

      Inter-assay Precision

      Sample

      1

      2

      3

      1

      2

      3

      n

      20

      20

      20

      20

      20

      20

      mean(ng/mL)

      0.41

      1.07

      4.97

      0.47

      1.25

      4.24

      Standard deviation

      0.02

      0.05

      0.22

      0.03

      0.06

      0.2

      CV (%)

      4.88

      2 4.67

      3 4.43

       6.38

      4.8

       4.72


      Recovery

      The recovery of TGF-β1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type

      Range (%)

      Average Recovery (%)

      Serum (n=5)

      94-110

      102

      EDTA plasma (n=5)

      90-102

      96

      Cell culture media (n=5)

      95-106

      98


      Linearity

      Samples were spiked with high concentrations of TGF-β1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

       

       

      Serum (n=5)

      EDTA plasma (n=5)

      Cellculturemedia(n=5)

      1:2

      Range (%)

      99-108

      95-105

      92-104

      Average (%)

      105

      102

      97

      1:4

      Range (%)

      95-109

      90-100

      97-105

      Average (%)

      102

      96

      100

      1:8

      Range (%)

      89-103

      89-104

      96-108

      Average (%)

      97

      97

      102

      1:16

      Range (%)

      88-102

      98-108

      92-105

      Average (%)

      94

      104

      100


      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

      Item

      Specifications

      Storage

      Micro ELISA Plate (Dismountable)

      8 wells ×12 strips

      -20, 6 months

      Reference Standard

      2 vials

      Concentrated Biotinylated Detection Ab (100×)

      1 vial, 120 uL

      Concentrated HRP Conjugate (100×)

      1 vial, 120 μL

      -20(shading light), 6 months

      Reference Standard & Sample Diluent

      1 vial, 20 mL

      4, 6 months

      Biotinylated Detection Ab Diluent

      1 vial, 14 mL

      HRP Conjugate Diluent

      1 vial, 14 mL

      Concentrated Wash Buffer (25×)

      1 vial, 30 mL

      Activator reagent 1 (1M HCL)

      1 vial, 5 mL

      Room temperature, 6 months

      Activator reagent 2 (1.2M NaOH/ 0.5M HEPES)

      1 vial, 5 mL

      Substrate Reagent

      1 vial, 10 mL

      4(shading light)

      Stop Solution

      1 vial, 10 mL

      4

      Plate Sealer

      5 pieces

       

      Product Description

      1 copy

      Certificate of Analysis

      1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.

      The volume of reagents in partial shipments is a little more than the volume marked on the label,    please use accurate measuring equipment instead of directly pouring.


      Other supplies required

      Microplate reader with 450 nm wavelength filter

      High-precision transfer pipette, EP tubes and disposable pipette tips

      37℃ Incubator

      Deionized or distilled water

      Absorbent paper

      Loading slot for Wash Buffer


      Assay Procedure


      Citations

      1. Effects And Mechanisms Of Geniposide On Rats With Adjuvant Arthritis.
        IF: 2.956
        Journal: International Immunopharmacology(2014)
        DOI: 10.1016/j.intimp.2014.02.021
        PMID: 24583144
        Products: E-EL-R0014 E-EL-R0015 E-EL-R0559 E-EL-0162 
        Sample type: Cell lysate
        Reactivity: Rat
      2. Galectin-9 Ameliorates Respiratory Syncytial Virus-Induced Pulmonary Immunopathology Through Regulating The Balance Between Th17 And Regulatory T Cells.
        IF: 2.628
        Journal: Virus Research(2014)
        DOI: 10.1016/j.virusres.2014.10.011
        PMID: 25451068
        Products: E-EL-R0016 E-EL-R0566 E-EL-0162 E-EL-R0009 
        Sample type: Tissue homogenate
        Reactivity: Rat
      3. Anti-Oxidant And Anti-Inflammatory Effects Of Apigenin In A Rat Model Of Sepsis: An Immunological, Biochemical, And Histopathological Study.
        IF: 1.475
        Journal: Immunopharmacology & Immunotoxicology(2016)
        DOI: 10.3109/08923973.2016.1173058
        PMID: 27144896
        Products: E-EL-R0012 E-EL-R0015 E-EL-R0016 E-EL-0162 
        Sample type: Serum
        Reactivity: Rat
      4. Suppression For Lung Metastasis By Depletion Of Collagen I And Lysyl Oxidase Via Losartan Assisted With Paclitaxel-Loaded Ph-Sensitive Liposomes In Breast Cancer.
        IF: 6.402
        Journal: Drug Delivery(2016)
        DOI: 10.3109/10717544.2015.1132798
        PMID: 26758229
        Products: E-EL-0162 
        Sample type: Cell lysate
        Reactivity: Human
      5. Concentrated Growth Factor Promotes Schwann Cell Migration Partly Through The Integrin Β1-Mediated Activation Of The Focal Adhesion Kinase Pathway.
        IF: 2.341
        Journal: International Journal of Molecular Medicine(2016)
        DOI: 10.3892/ijmm.2016.2520
        PMID: 26986804
        Products: E-EL-0162 
        Sample type: Cell culture supernatant
        Reactivity: Rat
      6. Pirfenidone Suppresses MAPK Signaling Pathway To Reverse Epithelial-Mesenchymal Transition And Renal Fibrosis.
        IF: 1.563
        Journal: Nephrology(2016)
        DOI: 10.1111/nep.12831
        PMID: 27245114
        Products: E-EL-0162 E-AB-33323 E-AB-31262 E-AB-14010 E-AB-32461 E-AB-13810 E-AB-20915 E-AB-20058 
        Sample type: Tissue homogenate
        Reactivity: Rat
      7. Inhibition Of Hepatocyte Apoptosis: An Important Mechanism Of Corn Peptides Attenuating Liver Injury Induced By Ethanol.
        IF: 3.226
        Journal: International Journal of Molecular Sciences(2015)
        DOI: 10.3390/ijms160922062
        PMID: 26378531
        Products: E-EL-M0049 E-EL-0162 
        Sample type: Serum,Tissue homogenate
        Reactivity: Mouse
      8. Foxp3+ Associated With The Pro-Inflammatory Regulatory T And T Helper 17 Effector Cells In Asthma Patients.
        IF: 1.261
        Journal: Experimental and Therapeutic Medicine(2016)
        DOI: 10.3892/etm.2016.3662
        PMID: 27703517
        Products: E-EL-0162 
        Sample type: Plasma
        Reactivity: Human
      9. Edaravone Attenuates Lipopolysaccharide-induced Acute Respiratory Distress Syndrome Associated Early Pulmonary Fibrosis Via Inhibition Of Oxidative Stress And Transforming Growth Factor-β1/Smad3 Signaling.
        IF: 2.466
        Journal: Biochemical And Biophysical Research Communications(2017)
        DOI: 10.1016/j.bbrc.2017.10.165
        PMID: 29102631
        Products: E-EL-0162 E-EL-R0015 
        Sample type: Serum
        Reactivity: Rat
      10. Two Sulfur Glycoside Compounds Isolated From Lepidium Apetalum Willd Protect NRK52e Cells Against Hypertonic-Induced Adhesion And Inflammation By Suppressing The MAPK Signaling Pathway And RAAS.
        IF: 2.861
        Journal: Molecules(2017)
        DOI: 10.3390/molecules22111956
        PMID: 29137154
        Products: E-EL-R0030 E-EL-R2401 E-EL-R1430 E-EL-R0633 E-EL-0162 E-EL-R0815 E-EL-0070 E-EL-R0792 E-EL-R0893 E-EL-R3012 
        Sample type: Cell culture supernatant
        Reactivity: Rat, Universal
      11. Long Non-Coding RNA-ATB Promotes EMT During Silica-Induced Pulmonary Fibrosis By Competitively Binding miR-200c.
        IF: 5.476
        Journal: Biochimica Et Biophysica Acta-Molecular Basis of Disease(2017)
        DOI: 10.1016/j.bbadis.2017.11.003
        PMID: 29113749
        Products: E-EL-0162 
        Sample type: Cell culture supernatant
        Reactivity: Human
      12. Effect Of Qianghuo Erhuang Decoction On T Regulatory And T Helper 17 Cells In Treatment Of Adjuvant-Induced Arthritis In Rats.
        IF: 4.259
        Journal: Scientific reports(2017)
        DOI: 10.1038/s41598-017-17566-w
        PMID: 29222448
        Products: E-EL-0162 E-EL-R0015 E-EL-R0566 
        Sample type: Serum
        Reactivity: Rat
      13. Aspirin Reduces Cardiac Interstitial Fibrosis by Inhibiting Erk1/2-Serpine2 and P-Akt Signalling Pathways.
        IF: 5.104
        Journal: Cellular Physiology and Biochemistry(2018)
        DOI: 10.1159/000487972
        PMID: 29518782
        Products: E-EL-M0248 E-EL-0162 
        Reactivity: Mouse
      14. Leukocyte Depletion Does Not Affect The In Vitro Healing Ability Of Platelet Rich Plasma.
        Journal: Experimental and Therapeutic Medicine(2018)
        DOI: 10.3892/etm.2018.5887
        PMID: 29556269
        Products: E-EL-H0108 E-EL-H1577 E-EL-H1575 E-EL-H1670 E-EL-0162 E-EL-H0109 E-EL-H0092 
        Reactivity: Human
      15. Cordyceps Cicadae NTTU 868 Mycelium Prevents CCl 4-Induced Hepatic Fibrosis In BALB/c Mice Via Inhibiting The Expression Of Pro-Inflammatory And Pro-Fibrotic Cytokines.
        IF: 3.144
        Journal: Journal of Functional Foods(2018)
        DOI: 10.1016/j.jff.2018.02.010
        Products: E-EL-0162 E-EL-M0340 E-EL-M2434 E-EL-M0784 E-EL-M1165 
        Reactivity: Mouse
      16. Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.
        IF: 3.457
        Journal: Biomedicine & Pharmacotherapy(2018)
        DOI: 10.1016/j.biopha.2018.07.081
        PMID: 30119204
        Products: E-EL-R0009 E-EL-R0012 E-EL-R0014 E-EL-R0015 E-EL-R0039 E-EL-0162 E-EL-R3009 E-EL-R0566 E-EL-R1012 E-EL-R1096 
        Sample type: Serum
        Reactivity: Rat
      17. SMOC1 silencing suppresses the angiotensin IIinduced myocardial fibrosis of mouse myocardial fibroblasts via affecting the BMP2/Smad pathway.
        IF: 1.664
        Journal: Oncology Letters(2018)
        DOI: 10.3892/ol.2018.8989
        PMID: 30127878
        Products: E-EL-0162 E-EL-M0325 E-EL-M0316 
        Sample type: Plasmid
        Reactivity: Mouse
      18. Gap junction-mediated MiR-200b on osteogenesis and angiogenesis in coculture between MSCs and HUVECs.
        IF: 4.401
        Journal: J Cell Sci(2018)
        DOI: 10.1242/jcs.216135
        PMID: 29898921
        Products: E-EL-R2603 E-EL-R0854 E-EL-R3027 E-EL-0162 
        Sample type: Tissue homogenate
        Reactivity: Rat
      19. Mechanistic Insight Into the Efficient Osteogenic Potential of Dihydrotestosterone: Exploring Sequential Expression of Bone-Related Protein Biomarkers.
        IF: /
        Journal: Acta Medica Iranica(2018)
        Products: E-EL-H1347 E-EL-0162 E-EL-H0786 E-EL-H2500 
        Sample type: Cells
        Reactivity: Mouse
      20. Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS.
        IF: 3.098
        Journal: Molecules(2017)
        DOI: 10.3390/molecules22111956
        PMID: 29137154
        Products: E-EL-R0030 E-EL-R2401 E-EL-R1430 E-EL-0070 E-EL-R0633 E-EL-0162 E-EL-R3012 E-EL-R0792 E-EL-R0893 
        Sample type: Cell culture supernatant
        Reactivity: Rat
      21. The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice.
        IF: 3.273
        Journal: Toxins(2018)
        DOI: 10.3390/toxins10110449
        PMID: 30384460
        Products: E-EL-R3009 E-EL-0162 E-EL-R0012 E-EL-R0015 E-EL-R0014 E-EL-R0566 E-EL-R0009 
        Sample type: Serum, Tissue homogenate
        Reactivity: Mouse
      22. Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites
        IF: 4.471
        Journal: International Journal of Nanomedicine(2019)
        DOI: 10.2147/IJN.S184920
        PMID: 30666115
        Products: E-EL-R2603 E-EL-0162 
        Sample type: Cell culture supernatant
        Reactivity: Rat
      23. Pterostilbene alleviates fructose-induced renal fibrosis by suppressing TGF- β1/TGF-β type I receptor/Smads signaling in proximal tubular epithelial cells
        IF: 3.04
        Journal: European Journal of Pharmacology(2018)
        DOI: 10.1016/j.ejphar.2018.10.008
        PMID: 30336139
        Products: E-EL-0162 
        Sample type: Serum,Cell culture supernatant
        Reactivity: Human
      24. A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility
        IF: 6.638
        Journal: Acta Biomaterialia(2019)
        DOI: 10.1016/j.actbio.2019.05.012
        PMID: 31075515
        Products: E-EL-H0111 E-EL-0162 E-EL-H1577 
        Sample type: Cell culture supernatant
        Reactivity: Human
      25. Dual-Locking Nanoparticles Disrupt the PD-1/PD-L1 Pathway for Efficient Cancer Immunotherapy
        IF: 25.809
        Journal: Advanced materials(2019)
        DOI: 10.1002/adma.201905751
        PMID: 31709671
        Products: E-EL-0162 E-EL-M0048 E-EL-M0049 E-EL-M0042 E-EL-M0726 
        Sample type: Tissue homogenate
        Reactivity: Mouse
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