TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit
Cartilage-inducing factor、CED、Differentiation inhibiting factor、DPD1、LAP、Latency-associated peptide、Prepro transforming growth factor beta 1、TGF beta 1、TGF beta、TGF beta 1 protein、TGF-beta 1 protein、TGF-beta-1、TGF-beta-5、TGF-beta1、TGFB、Tgfb-1、tgfb1、TGFB1、TGFbeta、TGFbeta1、Transforming Growth Factor b1、Transforming Growth Factor beta 1、Transforming growth factor beta 1a、transforming growth factor beta-1、transforming growth factor、beta 1、Transforming Growth Factor-ß1
Price: $ 495
Price: $ 396
Price: $ 150
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Price: Inquire
- Reactivity: Universal
- Detection Range: 0.16-10 ng/mL
- Sensitivity: 0.1 ng/mL
For research use only. Order now, ship in 3 days
Test Principle | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Universal TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Universal TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Universal TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Universal TGF-β1. You can calculate the concentration of Universal TGF-β1 in the samples by comparing the OD of the samples to the standard curve. |
Assay Type | Sandwich-ELISA |
size | 96T / 48T / 24T / 96T*10 / 96T*5 |
Assay Time | 3.5h |
Detection Method | Colormetric |
Detection Range | 0.16-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Sample Volume | 100μL |
Sample Type | Serum, plasma and other biological fluids |
Specificity | This kit recognizes Universal TGF-β1 in samples.No significant cross-reactivity or interference between Universal TGF-β1 and analogues was observed |
Precision | Both intra-CV and inter-CV are < 10%. |
Recovery | 80%-120% |
Introduction | This ELISA kit applies to the in vitro quantitative determination of TGF-β1 concentrations in Serum, plasma and other biological fluids. |
Applications | ELISA |
Storage | 2-8℃/-20℃ |
Database | SwissProt: H:P01137 M:P04202 R:P17246 |
Syonoyms | Cartilage-inducing factor,CED,Differentiation inhibiting factor,DPD1,LAP,Latency-associated peptide,Prepro transforming growth factor beta 1,TGF beta 1,TGF beta,TGF beta 1 protein,TGF-beta 1 protein,TGF-beta-1,TGF-beta-5,TGF-beta1,TGFB,Tgfb-1,tgfb1,TGFB1,TGFbeta,TGFbeta1,Transforming Growth Factor b1,Transforming Growth Factor beta 1,Transforming growth factor beta 1a,transforming growth factor beta-1,transforming growth factor,beta 1,Transforming Growth Factor-ß1 |
Research Area | Cancer , Cardiovascular , Metabolism , Signal Transduction , Stem Cells |
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Q1: Why tissue samples do not need to be activated when TGF- beta (transforming growth factor - beta) was detected;
Since the antibodies in the kit recognize activated TGF-β, and TGF-β is a disulfide bond linked by two identical or similar 12.5kDa subunits of molecular weight. The study of human TGF-β cDNA sequence showed that the 112 amino acid residues of the monomer TGF-β were cleaved from the carboxyl terminal by a precursor molecule (per-pro-TGF-β) containing 400 amino acid residues. The N-terminal of per-pro-TGF-β contains a signal peptide, which is cleaved before secretion to become an inactive polypeptide chain precursor (pro-TGF-β). The n-terminal part of the amino acid residue is removed by changing the ionic strength, acidification or protease hydrolysis, and the remaining carboxyl terminal part forms an active TGF-β. A variety of cells in the body can secrete TGF-β in an inactive state. In vitro, the inactive TGF-β, also known as latency associated peptide (LAP), can be activated by acidification. In vivo, the acidic environment can be present near fractures and healing wounds, and the cleavage of the protein itself can cause the TGF-β complex to become activated TGF-β. In general, tissues with active cell differentiation often contain high levels of TGF-β, such as osteoblasts, kidney, bone marrow, and fetal liver hematopoietic cells. Therefore, no additional activation processing is required for tissue sample testing.
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Q2:What are the specifications of ELISA Kit?
There are four specifications of 96T/48T/24T/96T*5. Among them, 24T is the trial size.
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Q3:Why do we recommend pre-testing? How to conduct a pre-experiment?
The purpose of the pre-test is: ① to determine whether the kit is suitable for your sample detection, so as to avoid the waste of kits and samples; ② To help you get familiar with the operating process of the kit, especially the customers who use the ELISA kit for the first time/change the experimental brand; ③ Determine whether the sample is diluted or not and whether the dilution ratio is suitable for the determination of this kit and confirm the optimal sample dilution information; ④ Help to understand the experimental phenomena or problems that may occur during the experiment, so as to make timely adjustments; Specific methods: Before the formal test, 2-3 samples with large differences are selected to dilute the samples with different concentrations, and then pre-test is conducted according to the experimental procedures in the instructions. According to the results of the pre-test, the optimal dilution ratio of the samples is determined combined with the detection range of the kit.
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Q4:What species can the ELISA Kit detect?
At present, the species detected by our ELISA kit are mainly human, mouse, rat and a small number of targets involve special species such as monkey, rabbit, pig and chicken. Kits with the name of a particular species (such as human) are specific to that species. If no species is specified in the manual, the kit is generic within the conventional universal species. If the species tested is special, it is recommended to confirm the suitability of the sample with technical support first.
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Q5:How to operate the "repeated freeze-thaw" process mentioned in tissue or cell sample processing?
Tissue sample: After finishing tissue grinding, place it at -80℃ for 1h/ liquid nitrogen for 0.5h, and then gently shake it in a water bath at 30℃ to melt it quickly. Repeat this operation 1-2 times. Cell sample: Repeat the above freeze-thaw operation 2-3 times. If it is a membrane protein, it can be appropriately sonicated, but the temperature and frequency of ultrasound need to be controlled. It is recommended to add protease inhibitors to the sample in advance. PMSF(Cat.E-EL-SR002) is generally recommended.
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Q6:Can the standard curve in the manual be used directly for calculation?
The standard curve on the manual is an indicator curve, mainly used to show the shape of the standard curve after fitting and the quality control range of the highest/lowest OD value, you can not directly use. In addition, due to the influence of experimental operation, experimental environment, instrument parameter setting and other factors, the OD value of your actual standard curve may not be completely consistent with the instructions (overall high or low). In this case, it is recommended to control the first 4 blue wells in the color development stage with obvious color gradient and the maximum OD value of the standard curve above 1.2. If the correlation coefficient of the standard tune is above 0.99, it can be used as an effective standard curve.
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Q7:The sample/reagent volume is not enough, can I reduce the sample volume uniformly?
Neither can be reduced. The sample volume of each step should be strictly in accordance with the instructions, otherwise the experimental system will be changed, and the results will be inaccurate. If the customer really does not have enough sample volume, you can consult the technology to confirm whether the sample can be diluted, and judge the sample concentration through pre-experiment Degree situation. If the volume of the reagent is not enough, the number of holes to be tested can only be reduced according to the actual volume, and the detection can not be diluted.
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Q8:How long was the ELISA test?
Traditional Competion Method: about 2.0 h; Traditiona three-steps Sandwich method: about 3.5h; QuicKey ELISA: about 2.5h; QuicKey Pro ELISA: about 1.5h.
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Q9:Does ELISA require technical replication? How to set the positive or negative control?
It is recommended that customers do technical repetition, on the one hand can confirm the operation method, on the other hand can verify and improve the accuracy of the experimental results. A positive control in an ELISA test can be considered the standard product. Negative control is a blank matrix or a matrix solution with known low concentration that has homology and homogeneity with the object to be picked up, which is generally difficult to obtain, and can be set as a negative control if available. The routine is to set a blank control, that is, a sample diluent.
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Q10:Why should a product with two item numbers of a target test different sample types?
Different sample types of matrix are different, in order to reduce the influence of the matrix itself and improve the accuracy of routine sample measurement, usually set different systems of ELISA kits, and display with different product numbers. In addition, the concentration of some indicators varies greatly between different samples, so different products are used to meet customers' needs for ease of operation.