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TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit

  • Cat.No.:E-EL-0162

  • Reactivity: Universal

To Purchase E-EL-0162

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495


Format 24T, 48T, 96T, 96T*5, 96T*10
Assay time 3.5h
Sample type Serum, plasma and other biological fluids
Sample volume 100μL
Sample size Run up to 46 samples in duplicate and 30 samples in triplicate
Storage 2-8℃
Interpretation Detection range: 0.16-10 ng/mL
Sensitivity: 0.10 ng/mL
Application Quantitative determination of Human S1RBD concentrations in human serum, plasma and other biological fluids
Reproducibility Both intra-CV and inter-CV are < 10%
Synonyms Cartilage-inducing factor,CED,Differentiation inhibiting factor,DPD1,LAP,Latency-associated peptide,Prepro transforming growth factor beta 1,TGF beta 1,TGF beta,TGF beta 1 protein,TGF-beta 1 protein,TGF-beta-1,TGF-beta-5,TGF-beta1,TGFB,Tgfb-1,tgfb1,TGFB1,TGFbeta,TGFbeta1,Transforming Growth Factor b1,Transforming Growth Factor beta 1,Transforming growth factor beta 1a,transforming growth factor beta-1,transforming growth factor,beta 1,Transforming Growth Factor-ß1

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Universal TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Universal TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Universal TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Universal TGF-β1. You can calculate the concentration of Universal TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


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  1. Advanced materials (2019) IF: 27.398
    Dual-Locking Nanoparticles Disrupt the PD-1/PD-L1 Pathway for Efficient Cancer Immunotherapy

    DOI: 10.1002/adma.201905751

    PMID: 31709671

    Sample: Tissue homogenate
  2. Biomaterials (2020) IF: 10.317
    Virus-like nanoparticle as a co-delivery system to enhance efficacy of CRISPR/Cas9-based cancer immunotherapy

    DOI: 10.1016/j.biomaterials.2020.120275

    PMID: 99999999

    Sample: Serum,Cell lysate,Tissue homogenate
  3. Acta Biomaterialia (2019) IF: 6.638
    A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility

    DOI: 10.1016/j.actbio.2019.05.012

    PMID: 31075515

    Sample: Cell culture supernatant
  4. Drug Delivery (2016) IF: 6.402
    Suppression For Lung Metastasis By Depletion Of Collagen I And Lysyl Oxidase Via Losartan Assisted With Paclitaxel-Loaded Ph-Sensitive Liposomes In Breast Cancer.

    DOI: 10.3109/10717544.2015.1132798

    PMID: 26758229

    Sample: Cell lysate
  5. Stem Cell Research & Therapy (2020) IF: 5.116
    Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model

    DOI: 10.1186/s13287-020-01733-4

    PMID: 32456711

    Sample: Plasma
  6. Cellular Physiology and Biochemistry (2018) IF: 5.104
    Aspirin Reduces Cardiac Interstitial Fibrosis by Inhibiting Erk1/2-Serpine2 and P-Akt Signalling Pathways.

    DOI: 10.1159/000487972

    PMID: 29518782

  7. Biochimica Et Biophysica Acta-Molecular Basis of Disease (2017) IF: 5.476
    Long Non-Coding RNA-ATB Promotes EMT During Silica-Induced Pulmonary Fibrosis By Competitively Binding miR-200c.

    DOI: 10.1016/j.bbadis.2017.11.003

    PMID: 29113749

    Sample: Cell culture supernatant
  8. International Journal of Nanomedicine (2019) IF: 4.471
    Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites

    DOI: 10.2147/IJN.S184920

    PMID: 30666115

    Sample: Cell culture supernatant
  9. J Cell Sci (2018) IF: 4.401
    Gap junction-mediated MiR-200b on osteogenesis and angiogenesis in coculture between MSCs and HUVECs.

    DOI: 10.1242/jcs.216135

    PMID: 29898921

    Sample: Tissue homogenate
  10. Scientific reports (2017) IF: 4.259
    Effect Of Qianghuo Erhuang Decoction On T Regulatory And T Helper 17 Cells In Treatment Of Adjuvant-Induced Arthritis In Rats.

    DOI: 10.1038/s41598-017-17566-w

    PMID: 29222448

    Sample: Serum
  11. Life Sciences (2020) IF: 3.647
    DHCR24 overexpression modulates microglia polarization and inflammatory response via Akt/GSK3β signaling in Aβ 25-35 treated BV-2 cells

    DOI: 10.1016/j.lfs.2020.118470

    PMID: 32950573

    Sample: Cell sample
  12. American Journal of Translational Research (2020) IF: 3.375
    Effects of catalpol on asthma by airway remodeling via inhibiting TGF-β1 and EGF in ovalbumin-induced asthmatic mice

    PMID: 32774762

    Sample: Serum
  13. Mediators of Inflammation (2020) IF: 3.758
    I-alowCD11bhigh DC Regulates the Immune Response in the Eyes of Experimental Autoimmune Uveitis

    DOI: 10.1155/2020/6947482

    PMID: 32256194

    Sample: Serum,Cell culture supernatant,Aqueous Humor
  14. International Immunopharmacology (2020) IF: 3.943
    Molecular hydrogen attenuates sepsis-induced neuroinflammation through regulation of microglia polarization through an mTOR-autophagy-dependent pathway

    DOI: 10.1016/j.intimp.2020.106287

    PMID: 32058932

    Sample: Tissue homogenate
  15. Life sciences (2019) IF: 3.647
    Reciprocal enhancement of thrombosis by endothelial-to-mesenchymal transition induced by iliac vein compression

    DOI: 10.1016/j.lfs.2019.116659

    PMID: 31323274

    Sample: Serum
  16. Inflammation (2019) IF: 3.212
    The Hypermethylation of Foxp3 Promoter Impairs the Function of Treg Cells in EAP

    DOI: 10.1007/s10753-019-01030-0

    PMID: 31209730

    Sample: Serum
  17. Biomedicine & Pharmacotherapy (2019) IF: 3.457
    Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro

    DOI: 10.1016/j.biopha.2019.108706

    PMID: 30844656

    Sample: Wound drainage fluid
  18. European Journal of Pharmacology (2018) IF: 3.04
    Pterostilbene alleviates fructose-induced renal fibrosis by suppressing TGF- β1/TGF-β type I receptor/Smads signaling in proximal tubular epithelial cells

    DOI: 10.1016/j.ejphar.2018.10.008

    PMID: 30336139

    Sample: Serum,Cell culture supernatant
  19. Toxins (2018) IF: 3.273
    The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice.

    DOI: 10.3390/toxins10110449

    PMID: 30384460

    Sample: Serum, Tissue homogenate
  20. Molecules (2017) IF: 3.098
    Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS.

    DOI: 10.3390/molecules22111956

    PMID: 29137154

    Sample: Cell culture supernatant
  21. Biomedicine & Pharmacotherapy (2018) IF: 3.457
    Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.

    DOI: 10.1016/j.biopha.2018.07.081

    PMID: 30119204

    Sample: Serum
  22. Journal of Functional Foods (2018) IF: 3.144
    Cordyceps Cicadae NTTU 868 Mycelium Prevents CCl 4-Induced Hepatic Fibrosis In BALB/c Mice Via Inhibiting The Expression Of Pro-Inflammatory And Pro-Fibrotic Cytokines.

    DOI: 10.1016/j.jff.2018.02.010

  23. International Journal of Molecular Sciences (2015) IF: 3.226
    Inhibition Of Hepatocyte Apoptosis: An Important Mechanism Of Corn Peptides Attenuating Liver Injury Induced By Ethanol.

    DOI: 10.3390/ijms160922062

    PMID: 26378531

    Sample: Serum,Tissue homogenate
  24. Future Microbiology (2020) IF: 2.907
    The anti-inflammatory, anti-oxidant and protective effects of a probiotic mixture on organ toxicity in a rat model

    DOI: 10.2217/fmb-2020-0005

    PMID: 32250184

    Sample: Serum
  25. Journal of the American College of Nutrition (2019) IF: 2.175
    A Peptide from Kiwifruit Exerts Anti-Inflammatory Effects in Celiac Disease Mucosa

    DOI: 10.1080/07315724.2018.1541426

    PMID: 30794064

    Sample: Tissue homogenate
  26. Molecules (2017) IF: 2.861
    Two Sulfur Glycoside Compounds Isolated From Lepidium Apetalum Willd Protect NRK52e Cells Against Hypertonic-Induced Adhesion And Inflammation By Suppressing The MAPK Signaling Pathway And RAAS.

    DOI: 10.3390/molecules22111956

    PMID: 29137154

    Sample: Cell culture supernatant
  27. Biochemical And Biophysical Research Communications (2017) IF: 2.466
    Edaravone Attenuates Lipopolysaccharide-induced Acute Respiratory Distress Syndrome Associated Early Pulmonary Fibrosis Via Inhibition Of Oxidative Stress And Transforming Growth Factor-β1/Smad3 Signaling.

    DOI: 10.1016/j.bbrc.2017.10.165

    PMID: 29102631

    Sample: Serum
  28. International Journal of Molecular Medicine (2016) IF: 2.341
    Concentrated Growth Factor Promotes Schwann Cell Migration Partly Through The Integrin Β1-Mediated Activation Of The Focal Adhesion Kinase Pathway.

    DOI: 10.3892/ijmm.2016.2520

    PMID: 26986804

    Sample: Cell culture supernatant
  29. Virus Research (2014) IF: 2.628
    Galectin-9 Ameliorates Respiratory Syncytial Virus-Induced Pulmonary Immunopathology Through Regulating The Balance Between Th17 And Regulatory T Cells.

    DOI: 10.1016/j.virusres.2014.10.011

    PMID: 25451068

    Sample: Tissue homogenate
  30. International Immunopharmacology (2014) IF: 2.956
    Effects And Mechanisms Of Geniposide On Rats With Adjuvant Arthritis.

    DOI: 10.1016/j.intimp.2014.02.021

    PMID: 24583144

    Sample: Cell lysate
  31. Hepatobiliary & Pancreatic Diseases International (2018) IF: 1.5
    Histone deacetylase inhibitor suberoylanilide hydroxamic acid alleviates liver fibrosis by suppressing the transforming growth factor-β1 signal pathway

    DOI: 10.1016/j.hbpd.2018.09.013

    PMID: 30249543

    Sample: Serum
  32. Oncology Letters (2018) IF: 1.664
    SMOC1 silencing suppresses the angiotensin IIinduced myocardial fibrosis of mouse myocardial fibroblasts via affecting the BMP2/Smad pathway.

    DOI: 10.3892/ol.2018.8989

    PMID: 30127878

    Sample: Plasmid
  33. Experimental and Therapeutic Medicine (2016) IF: 1.261
    Foxp3+ Associated With The Pro-Inflammatory Regulatory T And T Helper 17 Effector Cells In Asthma Patients.

    DOI: 10.3892/etm.2016.3662

    PMID: 27703517

    Sample: Plasma
  34. Nephrology (2016) IF: 1.563
    Pirfenidone Suppresses MAPK Signaling Pathway To Reverse Epithelial-Mesenchymal Transition And Renal Fibrosis.

    DOI: 10.1111/nep.12831

    PMID: 27245114

    Sample: Tissue homogenate
  35. Immunopharmacology & Immunotoxicology (2016) IF: 1.475
    Anti-Oxidant And Anti-Inflammatory Effects Of Apigenin In A Rat Model Of Sepsis: An Immunological, Biochemical, And Histopathological Study.

    DOI: 10.3109/08923973.2016.1173058

    PMID: 27144896

    Sample: Serum
  36. The Indonesian Biomedical Journal (2017)
    Comparison of Transforming Growth Factor-Beta 1 Concentration in Preeclampsia and Normal Pregnancy Women

    DOI: 10.18585/inabj.v9i1.310

    PMID: 99999999

    Sample: Serum
  37. Eurasian Journal of Biosciences (2020)
    Possible cause-and-effect linkage of transforming growth factor-beta1 and platelets derived growth factor-AB with delayed anthropometric parameters in adolescent patients with Cooley's anemia: Cases vis control research strategy

    PMID: 99999999

    Sample: Serum
  38. Eurasian Journal of Biosciences (2020)
    Relationship of levels of transforming growth factor-beta1 (TGF-β1) to the levels of ferritin in blood of transfusion dependent β-thalassemia major patients with growth retardation: A case-control study

    PMID: 99999999

    Sample: Serum
  39. Acta Medica Iranica (2018) IF: /
    Mechanistic Insight Into the Efficient Osteogenic Potential of Dihydrotestosterone: Exploring Sequential Expression of Bone-Related Protein Biomarkers.
    Sample: Cells
  40. Experimental and Therapeutic Medicine (2018)
    Leukocyte Depletion Does Not Affect The In Vitro Healing Ability Of Platelet Rich Plasma.

    DOI: 10.3892/etm.2018.5887

    PMID: 29556269

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