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TNF alpha Polyclonal Antibody

Cat:E-AB-33121 Citations (2)
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p;IF
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Product Details
Verified Samples Verified Samples in WB:Jurkat
Verified Samples in IF:Human stomach
Dilution

WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthesized peptide derived from the Internal region of human TNF-α
Abbre TNF-α
Synonyms APC1;APC1 protein;Cachectin;DIF;Differentiation inducing factor;Macrophage cytotoxic factor;Tnf;TNF superfamily;member 2;TNF;macrophage derived;TNF;monocyte derived;TNF-a;TNF-alpha;TNFA;TNFA;TNFSF2;Tumor necrosis factor alpha;Tumor necrosis factor;Tumor necrosis factor ligand superfamily member 2;Tumor Necrosis Factor;Membrane Form;Tumor necrosis factor;soluble form
Swissprot
Calculated MW 26kDa
Observed MW 16kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Secreted and Cell membrane.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer; Cardiovascular; Metabolism; Immunology; Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.