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Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit

Cat.No.:E-BC-K136-M
Detection instrument: Microplate reader(500-520 nm,optimum wavelength: 520 nm)

Detection method: Colorimetric method

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Detection principle

A variety of antioxidant macromolecules, antioxidant molecules and enzymes in a system can eliminate all kinds of reactive oxygen species and prevent oxidative stress induced by reactive oxygen species. The total level reflect the total antioxidant capacity in the system. Many antioxidants in the body can reduce Fe3+ to Fe2+ and Fe2+ can form stable complexes with phenanthroline substance. The antioxidant capacity (T-AOC) can be calculated by measuring the absorbance at 520 nm.

Performance characteristics

Synonyms	T-AOC
Sample type	Serum,plasma,whole blood,tissue,cells,cell culture supernatant
Sensitivity	0.62 U/mL
Detection range	0.62-190.43 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	50 min
Precision	Average inter-assay CV: 5.600%Average intra-assay CV: 4.800%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

The capacity of antioxidant was significantly increased by rhodoxanthin treatment. (***P<0.001)

D-A Dumitras et al investigate the antioxidant capacity of rhodoxanthin that can inhibit tumor growth. Total antioxidant capacity (T-AOC) in mouse plasma was determined using T-AOC colorimetric assay kit (E-BC-K136-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.62-190.43 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human urine	1-2
10% Rat liver tissue homogenate	1
10% Epipremnum aureum tissue homogenate	1
HepG2 cells	1
HepG2 cell culture supernatant	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025
Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B1

DOI: 10.1016/j.fct.2021.112124

PMID: 33727180
Sample: Tissue homogenate
Foods (2023) IF: 5.561
Treatment of Diabetes Nephropathy in Mice by Germinating Seeds of Euryale ferox through Improving Oxidative Stress

DOI: 10.3390/foods12040767

Sample: Serum
Frontiers in Pharmacology (2021) IF: 5.811
The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD

DOI: 10.3389/fphar.2021.709702

Sample: serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Comparison of Selenium Nanoparticles and Sodium Selenite on the Alleviation of Early Atherosclerosis by Inhibiting Endothelial Dysfunction and Inflammation in Apolipoprotein E-Deficient Mice

DOI: 10.3390/ijms222111612

PMID: 34769040
Sample: serum
Drug Design Development and Therapy (2022) IF: 4.319
The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

DOI: 10.2147/DDDT.S344247

PMID: 35965961
Sample: serum
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathway

DOI: 10.1016/j.jep.2021.114664

PMID: 34555451
Sample: lung tissue
Scientific Reports (2021) IF: 4.38
Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study

DOI: 10.1038/s41598-021-91256-6

PMID: 34083710
Sample: saliva
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells

DOI: 10.1016/j.biopha.2020.110684

Sample: serum
JOURNAL OF NUTRITION (2022) IF: 4.798
Brussels chicory stabilizes unstable atherosclerotic plaques and reshapes the gut microbiota in Apoe–/– mice

DOI: 10.1093/jn/nxac103

Sample: serum
Food Quality and Safety (2022) IF: 3.102
Novel Functional Food from an invasive species Polygonum cuspidatum: Safety evaluation, Chemical Composition, and Hepatoprotective Effects

DOI: 10.1093/fqsafe/fyac032
Biomed Research International (2022) IF: 3.246
Glycine Improved Cryopreserved Spermatozoa Quality in Achai Bull

DOI: 10.1155/2022/8282387

PMID: 35968237
Sample: semen
Frontiers in Veterinary Science (2022) IF: 3.412
Ovarian Oxidative Stress Induced Follicle Depletion After Zona Pellucida 3 Vaccination Is Associated With Subfertility in BALB/c Mice

DOI: 10.3389/fvets.2022.814827

PMID: 35252419
Sample: Ovarian
PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

DOI: 10.1080/13880209.2022.2070221

PMID: 35635784
Sample: brain tissue
Journal of Inorganic and Organometallic Polymers and Materials (2023) IF: 3.518
Preparation of Novel Composites of Polyvinyl Alcohol Containing Hesperidin Loaded ZnO Nanoparticles and Determination of Their Biological and Thermal Properties

DOI: 10.1007/s10904-023-02532-z
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 3.639
Flaxseed oil ameliorates aging in D-galactose induced rats via altering gut microbiota and mitigating oxidative damage

DOI: 10.1002/jsfa.12010

PMID: 35567370
Sample: serum,liver
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION (2022) IF: 3.682
Synergistic effect of ZnO nanoparticles and hesperidin on the antibacterial properties of chitosan

DOI: 10.1080/09205063.2022.2099668

PMID: 35797143
Sample: nanocomposites
Cancer Management and Research (2021) IF: 3.989
Cardioprotective Effect of Quercetin and Sitagliptin in Doxorubicin-Induced Cardiac Toxicity in Rats

DOI: 10.2147/CMAR.S300495

PMID: 33737832
Sample: Serum
International Journal of General Medicine (2023) IF: 2.145
Hematological, Inflammatory, Coagulation, and Oxidative/Antioxidant Biomarkers as Predictors for Severity and Mortality in COVID-19: A Prospective Cohort-Study

DOI: 10.2147/IJGM.S402206

Sample: Serum
JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72
Effect of coconut water and milk on heat stress-induced gastrointestinal tract dysmotility in rats: Role of oxidative stress and inflammatory response

DOI: 10.1111/jfbc.14129

PMID: 35298033
Sample: gastric,jejunal, colonic
JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE (2020) IF: 1.642
Spirometric Outcomes and Oxidative Stress Among Cement Factory Workers: Results From a Cross-Sectional Study

DOI: 10.1097/JOM.0000000000001991

PMID: 32796257
Sample: serum
TOXICOLOGY AND INDUSTRIAL HEALTH (2020) IF: 1.708
Elevated, sustained, and yet reversible biotoxicity effects of lead on cessation of exposure: Melatonin is a potent therapeutic option:

DOI: 10.1177/0748233720937199

Sample: Plasma
Heliyon (2021)
Postnatally induced metabolic and oxidative changes associated with maternal high-fat consumption were mildly affected by Quercetin-3-O-rutinoside treatment in rats

DOI: 10.1016/j.heliyon.2021.e06847

Sample: placenta tissue,liver tissue
Clinical Nutrition ESPEN (2022)
Impacts of a nutrition education intervention in individuals with cardiometabolic risk: Protocol of a randomized controlled trial

DOI: 10.1016/j.clnesp.2022.03.037

Sample: plasma
International Journal of Food Science (2023)
Fabrication of Nutraceutical Beverage from Saffron (Crocus sativus L.) Extract and Studying Its Health Effects

DOI: 10.1155/2023/7130266

Sample: serum
Toxicology Reports (2019)
Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

DOI: 10.1016/j.toxrep.2019.06.009

Sample: plasma

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Reviews/Q&A

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Reviews
Q&A

M*****iSubmitted [ Aug 11 2022 ]

Asked: Can I use normal saline for tissue homogenization?

adminSubmitted [ Aug 11 2022 ]

Answered:

adminSubmitted [ Aug 11 2022 ]

Answered: Yes, you can use normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

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