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Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit

Cat.No.:E-BC-K136-M
Detection instrument: Microplate reader(500-520 nm,optimum wavelength: 520 nm)

Detection method: Colorimetric method

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Detection principle

A variety of antioxidant macromolecules, antioxidant molecules and enzymes in a system can eliminate all kinds of reactive oxygen species and prevent oxidative stress induced by reactive oxygen species. The total level reflect the total antioxidant capacity in the system. Many antioxidants in the body can reduce Fe3+ to Fe2+ and Fe2+ can form stable complexes with phenanthroline substance. The antioxidant capacity (T-AOC) can be calculated by measuring the absorbance at 520 nm.

Performance characteristics

Synonyms	T-AOC
Sample type	Serum,plasma,whole blood,tissue,cells,cell culture supernatant
Sensitivity	0.62 U/mL
Detection range	0.62-190.43 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	50 min
Precision	Average inter-assay CV: 5.600%Average intra-assay CV: 4.800%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

The capacity of antioxidant was significantly increased by rhodoxanthin treatment. (***P<0.001)

D-A Dumitras et al investigate the antioxidant capacity of rhodoxanthin that can inhibit tumor growth. Total antioxidant capacity (T-AOC) in mouse plasma was determined using T-AOC colorimetric assay kit (E-BC-K136-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.62-190.43 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human urine	1-2
10% Rat liver tissue homogenate	1
10% Epipremnum aureum tissue homogenate	1
HepG2 cells	1
HepG2 cell culture supernatant	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Reviews/Q&A

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Reviews
Q&A

M*****iSubmitted [ Aug 11 2022 ]

Asked: Can I use normal saline for tissue homogenization?

adminSubmitted [ Aug 11 2022 ]

Answered:

adminSubmitted [ Aug 11 2022 ]

Answered: Yes, you can use normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

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