Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K136-M

      Size:
      • 96T
      • 48T
      Qty:
      - +
      Price: $200
      Can detect 48 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(500-520 nm,optimum wavelength: 520 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Detection principle

      A variety of antioxidant macromolecules, antioxidant molecules and enzymes in a system can eliminate all kinds of reactive oxygen species and prevent oxidative stress induced by reactive oxygen species. The total level reflect the total antioxidant capacity in the system. Many antioxidants in the body can reduce Fe3+ to Fe2+ and Fe2+ can form stable complexes with phenanthroline substance. The antioxidant capacity (T-AOC) can be calculated by measuring the absorbance at 520 nm.

      Performance characteristics

      Synonyms T-AOC
      Sample type Serum,plasma,whole blood,tissue,cells,cell culture supernatant
      Sensitivity 0.62 U/mL
      Detection range 0.62-190.43 U/mL
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 50 min
      Precision Average inter-assay CV: 5.6%Average intra-assay CV: 4.8%
      Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample

      It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.62-190.43 U/mL).

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human serum

      1

      Human urine

      1-2

      10% Rat liver tissue homogenate

      1

      10% Epipremnum aureum tissue homogenate

      1

      HepG2 cells

      1

      HepG2 cell culture supernatant

      1

      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

      Citations

      1. Antioxidant Effect of Folic Acid and its Relation to Salivary Proteins and Oral Health
        Journal: Al-Rafidain Dental Journal(2020)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K136-M 
        Sample type: Saliva
        Reactivity: Human
      2. Detection of some antioxidant markers in saliva of patients with beta thalassemia major
        Journal: Al-Kufa University Journal for Biology(2019)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K136-M E-BC-K136-S E-EL-0026 
        Sample type: Serum,Saliva
        Reactivity: Human
      3. Antioxidant effect of vitamin D3 and its relation to salivary proteins and oral health
        Journal: Al-Rafidain Dental Journal(2020)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K136-M 
        Sample type: Saliva
        Reactivity: Human
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      MSDS for ELISA ISO 9001

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