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Cat.No.:E-BC-K136-M
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Synonyms | T-AOC |
Sample type | Serum,plasma,whole blood,tissue,cells,cell culture supernatant |
Sensitivity | 0.62 U/mL |
Detection range | 0.62-190.43 U/mL |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 50 min |
Precision | Average inter-assay CV: 5.600%Average intra-assay CV: 4.800% |
Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
The capacity of antioxidant was significantly increased by rhodoxanthin treatment. (***P<0.001)
D-A Dumitras et al investigate the antioxidant capacity of rhodoxanthin that can inhibit tumor growth. Total antioxidant capacity (T-AOC) in mouse plasma was determined using T-AOC colorimetric assay kit (E-BC-K136-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.62-190.43 U/mL).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Human urine |
1-2 |
10% Rat liver tissue homogenate |
1 |
10% Epipremnum aureum tissue homogenate |
1 |
HepG2 cells |
1 |
HepG2 cell culture supernatant |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
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PMID: 33727180 |
Sample: Tissue homogenate |
Sample: Serum |
Sample: serum |
PMID: 34769040 |
Sample: serum |
DOI: 10.2147/DDDT.S344247 |
|
PMID: 35965961 |
Sample: serum |
PMID: 34555451 |
Sample: lung tissue |
PMID: 34083710 |
Sample: saliva |
Sample: serum |
DOI: 10.1093/jn/nxac103 |
|
Sample: serum |
DOI: 10.1155/2022/8282387 |
|
PMID: 35968237 |
Sample: semen |
PMID: 35252419 |
Sample: Ovarian |
PMID: 35635784 |
Sample: brain tissue |
DOI: 10.1002/jsfa.12010 |
|
PMID: 35567370 |
Sample: serum,liver |
PMID: 35797143 |
Sample: nanocomposites |
DOI: 10.2147/CMAR.S300495 |
|
PMID: 33737832 |
Sample: Serum |
DOI: 10.2147/IJGM.S402206 |
|
Sample: Serum |
DOI: 10.1111/jfbc.14129 |
|
PMID: 35298033 |
Sample: gastric,jejunal, colonic |
PMID: 32796257 |
Sample: serum |
Sample: Plasma |
Sample: placenta tissue,liver tissue |
Sample: plasma |
DOI: 10.1155/2023/7130266 |
|
Sample: serum |
Sample: plasma |
M*****iSubmitted [ Aug 11 2022 ]
Asked: Can I use normal saline for tissue homogenization?
Reply
adminSubmitted [ Aug 11 2022 ]
Answered:
adminSubmitted [ Aug 11 2022 ]
Answered: Yes, you can use normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).