Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Enzyme Method) (E-BC-K219-M)
For research use only.
| Detection Principle | The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 414 nm or 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity. |
| Synonyms | T-AOC |
| Sample Type | Serum,Plasma,Urine,Saliva,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (405-425 nm) |
| Research Area | Oxidative Stress , Plant Physiology Series |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), 60% Ethanol |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.047 mmol/L |
| Detection Range | 0.047-1.50 mmol/L |
| Precision | inter-assay CV: 4.1% | intra-assay CV: 2.2% |
| Sample Volume | 10 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat brain tissue homogenization | 1 |
| 10% Rat liver tissue homogenization | 1 |
| 10% Rat kidney tissue homogenization | 1 |
| 10% Epipremnum aureum tissue homogenization | 1 |
| Human serum | 1 |
| Human saliva | 1 |
| Human urine | 1 |
| Rat serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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