Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K136-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $200
      Can detect 50 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(520 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Detection principle

      A variety of antioxidant macromolecules, antioxidant molecules and enzymes in a system can eliminate all kinds of reactive oxygen species and prevent oxidative stress induced by reactive oxygen species. The total level reflect the total antioxidant capacity in the system. Many antioxidants in the body can reduce Fe3+ to Fe2+ and Fe2+ can form stable complexes with phenanthroline substance. The antioxidant capacity (T-AOC) can be calculated by measuring the absorbance at 520 nm.

      Performance characteristics

      Synonyms T-AOC
      Sample type Serum,plasma,saliva,urine,tissue,cells
      Sensitivity 0.62 U/mL
      Detection range 0.62-145.2 U/mL
      Detection method Colorimetric method
      Assay type Quantitative
      Assay time 80 min
      Precision Average inter-assay CV: 8.2%Average intra-assay CV: 2.7%
      Other instruments required Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Citations

      1. Assessment of the Antioxidant Potential of Hypoestes rosea Leaf in Lead-acetate-induced Albino Rats
        Journal: Journal of Complementary and Alternative Medical Research(2020)
        DOI: 10.9734/jocamr/2020/v9i130135
        PMID: 99999999
        Products: E-BC-K019-S E-BC-K136-S 
        Sample type: Plasma
        Reactivity: Rat
      2. Assessment of the Antioxidant Potential of Hypoestes rosea Leafin Streptozotocin-induced Diabetic Albino Rats
        Journal: Journal of Complementary and Alternative Medical Research(2020)
        DOI: 10.9734/jocamr/2020/v9i430149
        PMID: 99999999
        Products: E-BC-K019-S E-BC-K136-S 
        Sample type: Serum
        Reactivity: Rat
      3. Detection of some antioxidant markers in saliva of patients with beta thalassemia major
        Journal: Al-Kufa University Journal for Biology(2019)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K136-M E-BC-K136-S E-EL-0026 
        Sample type: Serum,Saliva
        Reactivity: Human
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      • Reviews (0)
      • Q&A (4)
      Q

      S**** Submitted [ Mar 21 2021 ]

      Asked: Is there is a video how to use the kit?

      Reply

      A

      adminSubmitted [ Mar 21 2021 ]

      Answered: Hi Sadiq, The kit manual had been sent to your mailbox for reference. If any question about the experimental procedures, pls feel free to contact me. Britney britney@elabscience.com

      Q

      S****tSubmitted [ Mar 08 2019 ]

      Asked: This kit can use invertebrate hemolymph?

      Reply

      A

      adminSubmitted [ Mar 08 2019 ]

      Answered: Dear Serhat, Yes, the kit can use invertebrate hemolymph, if there is other question, please feel free to ask me. Regards, Cathy

      Q

      fSubmitted [ Dec 01 2018 ]

      Asked:  hi is this elisa kit suitable for detection of total antioxidant capacity in small intestine tissue of rat thank you

      Reply

      A

      adminSubmitted [ Dec 01 2018 ]

      Answered: This kit can dectect small intestine tissue sample.

      Q

      F***hSubmitted [ Nov 30 2018 ]

      Asked: Hi I want to ask if I want to measure Total antioxidant capacity in small intestine tissue of rat and number of test I require 75 sample can I use this kit and how many kit I require if I use kit for 100 assay thank you

      Reply

      A

      adminSubmitted [ Nov 30 2018 ]

      Answered: This kit of 100 assays could detect 50 samples with no duplication. So you need 2 kits of 100 assays if you won't test the samples in duplication.

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