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Cat.No.:E-BC-K225-M
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Synonyms | T-AOC |
Sample type | Serum,plasma,saliva,urine,tissue,cells,cell culture supernatant |
Sensitivity | 0.049 mmol/L |
Detection range | 0.049-2.5 mmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 20 min |
Precision | Average inter-assay CV: 8.100%Average intra-assay CV: 3.900% |
Other instruments required | Balance, Incubator, Micropipettor |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
No significant difference in total oxidant status between Phyllanthus emblica group and placebo group can be seen.
D Shanmugarajan et al investigate the antihypertensive and pleiotropic effect of Phyllanthus emblica extract. Total antioxidant capacity (T-AOC) in human serum was determined using T-AOC colorimetric assay kit (E-BC-K225-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.049-2.5 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Human saliva |
1 |
Human urine |
1 |
Cellular supernatant |
1 |
HepG2 cells homogenization |
1 |
5% Mouse liver tissue homogenization |
1 |
10% Epipremnum aureum tissue homogenization |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
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Sample: Serum |
Sample: serum |
PMID: 34769040 |
Sample: serum |
DOI: 10.2147/DDDT.S344247 |
|
PMID: 35965961 |
Sample: serum |
PMID: 34555451 |
Sample: lung tissue |
PMID: 34083710 |
Sample: saliva |
Sample: serum |
DOI: 10.1155/2022/8282387 |
|
PMID: 35968237 |
Sample: semen |
Sample: Tissue homogenate |
PMID: 35252419 |
Sample: Ovarian |
PMID: 35635784 |
Sample: brain tissue |
DOI: 10.1002/jsfa.12010 |
|
PMID: 35567370 |
Sample: serum,liver |
PMID: 35797143 |
Sample: nanocomposites |
DOI: 10.2147/IJGM.S402206 |
|
Sample: Serum |
DOI: 10.1111/ijfs.14529 |
|
Sample: coconut sap |
Sample: blood |
PMID: 32796257 |
Sample: serum |
Sample: fecal |
DOI: 10.1155/2023/7130266 |
|
Sample: serum |
Sample: plasma |
Sample: gastrointestinal tissue |
M*****iSubmitted [ Sep 20 2022 ]
Asked: What is the advantage of FRAP compared to other methods for detecting T-AOC?
Reply
adminSubmitted [ Sep 20 2022 ]
Answered: It has the easiest process with only two steps of operation and 5 min for incubation.