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Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP Method)

Catalog number:E-BC-K225-M

Size:

96T
48T

Price: $180

Can detect 80 samples if without duplication

Detection method: Colorimetric method

Detection instrument: Microplate reader(590-600 nm)

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Detection principle

Fe3+-TPTZ can be reduced by antioxidants and produce blue Fe2+-TPTZ under acid condition. The antioxidant capacity of sample can be calculated by detection the absorbance value at 593 nm.

Performance characteristics

Synonyms	T-AOC
Sample type	Serum,plasma,saliva,urine,tissue,cells,cell culture supernatant
Sensitivity	0.049 mmol/L
Detection range	0.049-2.5 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 8.1%Average intra-assay CV: 3.9%
Other instruments required	Balance, Incubator, Micropipettor
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	6 months

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