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Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP Method)

Cat.No.:E-BC-K225-M
Detection instrument: Microplate reader(590-600 nm)

Detection method: Colorimetric method

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Detection principle

Fe3+-TPTZ can be reduced by antioxidants and produce blue Fe2+-TPTZ under acid condition. The antioxidant capacity of sample can be calculated by detection the absorbance value at 593 nm.

Performance characteristics

Synonyms	T-AOC
Sample type	Serum,plasma,saliva,urine,tissue,cells,cell culture supernatant
Sensitivity	0.049 mmol/L
Detection range	0.049-2.5 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 8.100%Average intra-assay CV: 3.900%
Other instruments required	Balance, Incubator, Micropipettor
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

No significant difference in total oxidant status between Phyllanthus emblica group and placebo group can be seen.

D Shanmugarajan et al investigate the antihypertensive and pleiotropic effect of Phyllanthus emblica extract. Total antioxidant capacity (T-AOC) in human serum was determined using T-AOC colorimetric assay kit (E-BC-K225-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.049-2.5 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human saliva	1
Human urine	1
Cellular supernatant	1
HepG2 cells homogenization	1
5% Mouse liver tissue homogenization	1
10% Epipremnum aureum tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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DOI: 10.3390/foods12040767
	Sample: Serum

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The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD

DOI: 10.3389/fphar.2021.709702
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DOI: 10.3390/ijms222111612
PMID: 34769040	Sample: serum

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The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

DOI: 10.2147/DDDT.S344247
PMID: 35965961	Sample: serum

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Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathway

DOI: 10.1016/j.jep.2021.114664
PMID: 34555451	Sample: lung tissue

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DOI: 10.1038/s41598-021-91256-6
PMID: 34083710	Sample: saliva

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DOI: 10.1016/j.biopha.2020.110684
	Sample: serum

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DOI: 10.1155/2022/8282387
PMID: 35968237	Sample: semen

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-arginine reverses aging process through suppression of oxidative stress, inflammation, and apoptosis in the rat heart

DOI: 10.1007/s00424-019-02311-1
	Sample: Tissue homogenate

Frontiers in Veterinary Science (2022) IF: 3.412

Ovarian Oxidative Stress Induced Follicle Depletion After Zona Pellucida 3 Vaccination Is Associated With Subfertility in BALB/c Mice

DOI: 10.3389/fvets.2022.814827
PMID: 35252419	Sample: Ovarian

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DOI: 10.1080/13880209.2022.2070221
PMID: 35635784	Sample: brain tissue

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DOI: 10.1007/s10904-023-02532-z

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DOI: 10.1002/jsfa.12010
PMID: 35567370	Sample: serum,liver

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DOI: 10.1080/09205063.2022.2099668
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	Sample: Serum

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DOI: 10.1111/ijfs.14529
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DOI: 10.15537/smj.2021.42.6.20210019
	Sample: blood

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DOI: 10.1097/JOM.0000000000001991
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Reviews/Q&A

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Reviews
Q&A

M*****iSubmitted [ Sep 20 2022 ]

Asked: What is the advantage of FRAP compared to other methods for detecting T-AOC?

adminSubmitted [ Sep 20 2022 ]

Answered: It has the easiest process with only two steps of operation and 5 min for incubation.

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