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Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP Method)

Cat.No.:E-BC-K225-M
Detection instrument: Microplate reader(590-600 nm)

Detection method: Colorimetric method

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Detection principle

Fe3+-TPTZ can be reduced by antioxidants and produce blue Fe2+-TPTZ under acid condition. The antioxidant capacity of sample can be calculated by detection the absorbance value at 593 nm.

Performance characteristics

Synonyms	T-AOC
Sample type	Serum,plasma,saliva,urine,tissue,cells,cell culture supernatant
Sensitivity	0.049 mmol/L
Detection range	0.049-2.5 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 8.100%Average intra-assay CV: 3.900%
Other instruments required	Balance, Incubator, Micropipettor
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

No significant difference in total oxidant status between Phyllanthus emblica group and placebo group can be seen.

D Shanmugarajan et al investigate the antihypertensive and pleiotropic effect of Phyllanthus emblica extract. Total antioxidant capacity (T-AOC) in human serum was determined using T-AOC colorimetric assay kit (E-BC-K225-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.049-2.5 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human saliva	1
Human urine	1
Cellular supernatant	1
HepG2 cells homogenization	1
5% Mouse liver tissue homogenization	1
10% Epipremnum aureum tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Foods (2023) IF: 5.561
Treatment of Diabetes Nephropathy in Mice by Germinating Seeds of Euryale ferox through Improving Oxidative Stress

DOI: 10.3390/foods12040767

Sample: Serum
Frontiers in Pharmacology (2021) IF: 5.811
The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD

DOI: 10.3389/fphar.2021.709702

Sample: serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Comparison of Selenium Nanoparticles and Sodium Selenite on the Alleviation of Early Atherosclerosis by Inhibiting Endothelial Dysfunction and Inflammation in Apolipoprotein E-Deficient Mice

DOI: 10.3390/ijms222111612

PMID: 34769040
Sample: serum
Drug Design Development and Therapy (2022) IF: 4.319
The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

DOI: 10.2147/DDDT.S344247

PMID: 35965961
Sample: serum
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathway

DOI: 10.1016/j.jep.2021.114664

PMID: 34555451
Sample: lung tissue
Scientific Reports (2021) IF: 4.38
Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study

DOI: 10.1038/s41598-021-91256-6

PMID: 34083710
Sample: saliva
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells

DOI: 10.1016/j.biopha.2020.110684

Sample: serum
Food Quality and Safety (2022) IF: 3.102
Novel Functional Food from an invasive species Polygonum cuspidatum: Safety evaluation, Chemical Composition, and Hepatoprotective Effects

DOI: 10.1093/fqsafe/fyac032
Biomed Research International (2022) IF: 3.246
Glycine Improved Cryopreserved Spermatozoa Quality in Achai Bull

DOI: 10.1155/2022/8282387

PMID: 35968237
Sample: semen
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY (2019) IF: 3.377
-arginine reverses aging process through suppression of oxidative stress, inflammation, and apoptosis in the rat heart

DOI: 10.1007/s00424-019-02311-1

Sample: Tissue homogenate
Frontiers in Veterinary Science (2022) IF: 3.412
Ovarian Oxidative Stress Induced Follicle Depletion After Zona Pellucida 3 Vaccination Is Associated With Subfertility in BALB/c Mice

DOI: 10.3389/fvets.2022.814827

PMID: 35252419
Sample: Ovarian
PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

DOI: 10.1080/13880209.2022.2070221

PMID: 35635784
Sample: brain tissue
Journal of Inorganic and Organometallic Polymers and Materials (2023) IF: 3.518
Preparation of Novel Composites of Polyvinyl Alcohol Containing Hesperidin Loaded ZnO Nanoparticles and Determination of Their Biological and Thermal Properties

DOI: 10.1007/s10904-023-02532-z
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 3.639
Flaxseed oil ameliorates aging in D-galactose induced rats via altering gut microbiota and mitigating oxidative damage

DOI: 10.1002/jsfa.12010

PMID: 35567370
Sample: serum,liver
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION (2022) IF: 3.682
Synergistic effect of ZnO nanoparticles and hesperidin on the antibacterial properties of chitosan

DOI: 10.1080/09205063.2022.2099668

PMID: 35797143
Sample: nanocomposites
International Journal of General Medicine (2023) IF: 2.145
Hematological, Inflammatory, Coagulation, and Oxidative/Antioxidant Biomarkers as Predictors for Severity and Mortality in COVID-19: A Prospective Cohort-Study

DOI: 10.2147/IJGM.S402206

Sample: Serum
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (2020) IF: 2.773
Effect of processing method on vitamin profile, antioxidant properties and total phenolic content of coconut (Cocos nucifera L.) sugar syrup

DOI: 10.1111/ijfs.14529

Sample: coconut sap
SAUDI MEDICAL JOURNAL (2021) IF: 1.484
Effect of short duration moderate intensity physical activity on glycemic control and antioxidant status of prediabetic population.

DOI: 10.15537/smj.2021.42.6.20210019

Sample: blood
JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE (2020) IF: 1.642
Spirometric Outcomes and Oxidative Stress Among Cement Factory Workers: Results From a Cross-Sectional Study

DOI: 10.1097/JOM.0000000000001991

PMID: 32796257
Sample: serum
Livestock Science (2022) IF: 1.943
Effects of nature identical essential oils (carvacrol, thymol and cinnamaldehyde) on growth performance of piglets and non-invasive markers of antioxidant status and calprotectin release

DOI: 10.1016/j.livsci.2022.104959

Sample: fecal
International Journal of Food Science (2023)
Fabrication of Nutraceutical Beverage from Saffron (Crocus sativus L.) Extract and Studying Its Health Effects

DOI: 10.1155/2023/7130266

Sample: serum
Toxicology Reports (2019)
Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

DOI: 10.1016/j.toxrep.2019.06.009

Sample: plasma
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY (2020) IF: 0.579
Mild-intensity Exercise Triggers VEGF in the Digestive Tract Via Both Hypoxic and Nonhypoxic Mechanisms

DOI: 10.1590/1678-4324-2020200059

Sample: gastrointestinal tissue

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Reviews/Q&A

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Reviews
Q&A

M*****iSubmitted [ Sep 20 2022 ]

Asked: What is the advantage of FRAP compared to other methods for detecting T-AOC?

adminSubmitted [ Sep 20 2022 ]

Answered: It has the easiest process with only two steps of operation and 5 min for incubation.

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