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Total Carbonyl Colorimetric Assay Kit

Cat.No.:E-BC-K171-S
Detection instrument: Spectrophotometer(370 nm)

Detection method: Colorimetric method

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Detection principle

Carbonyl can react with 2,4-dinitrophenylhydrazine and produce a kind of reddish brown hydrazone compounds, which has a specific absorbance peak at 370 nm. The content of carbonyl can be calculated according to the absorbance value.

Performance characteristics

Sample type	Serum,plasma,,tissue
Sensitivity	0.94 μg/mL
Detection range	0.94-45 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 8.300%Average intra-assay CV: 4.400%
Other instruments required	Micropipettor, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

ARTIFICIAL ORGANS (2020) IF: 2.259
Cold‐inducible RNA binding protein agonist enhances the cardioprotective effect of UW solution during extended heart preservation

DOI: 10.1111/aor.13695

Sample: Tissue homogenate

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