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Cat.No.:E-BC-K171-M
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Protocols for metabolism assays
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Sample type | Serum,plasma,tissue |
Sensitivity | 1.29 μg/mL |
Detection range | 1.29-45 μg/mL |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 20 min |
Precision | Average inter-assay CV: 5%Average intra-assay CV: 1.900% |
Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.29-45 μg/mL).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Porcine serum |
1-2 |
Human serum |
1-2 |
Human plasma |
1-2 |
10% Rat kidney tissue homogenate |
3-5 |
10% Rat liver tissue homogenate |
3-5 |
10% Epipremnum aureum tissue homogenate |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).