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Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit

Citations(7)

Cat.No.:E-BC-K097-S
Detection instrument: Spectrophotometer(412 nm)

Detection method: Colorimetric method

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Detection principle

GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.

Performance characteristics

Synonyms	T-GSH,GSSG
Sample type	Serum,plasma,animal tissue,red blood cells,cultured cells
Sensitivity	0.12 μmol/L T-GSH
Detection range	0.12-30 μmol/L T-GSH
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	180 min
Precision	Average inter-assay CV: 4.700%Average intra-assay CV: 0.900%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required	PBS(0.01 M, pH 7.4), Absolute ethanol
Storage	Reagent 2, Reagent 3, Reagent 4, Reagent 8, Reagent 9: -20℃; others: 2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-30 μmol/L T-GSH).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Rat plasma	1
Mouse serum	1
10% Rat heart tissue homogenate	10
10% Rat liver tissue homogenate	60
10% Rat brain tissue homogenate	10

Note: The diluent is reagent 3.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Bioengineered (2022) IF: 3.269

SNAI2 promotes the development of ovarian cancer through regulating ferroptosis

DOI: 10.1080/21655979.2021.2024319
PMID: 35220872

PHARMACEUTICAL BIOLOGY (2022) IF: 3.503

Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

DOI: 10.1080/13880209.2022.2070221
PMID: 35635784	Sample: Brain Tissue

Porcine Health Management (2022) IF: 3.535

Is a new generation of mycotoxin clay adsorbents safe in a pig’s diet?

DOI: 10.1186/s40813-022-00275-w
	Sample: Blood

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2021) IF: 3.639

Effects of almonds on ameliorating salt-induced hypertension in Dahl salt-sensitive rats

DOI: 10.1002/jsfa.11611
PMID: 34708426	Sample: Renal Cortex

Food & Function (2021) IF: 5.396

Supplementation of amino acids and organic acids prevents the increase in blood pressure induced by high salt in Dahl salt-sensitive rats

DOI: 10.1039/D1FO03577K
PMID: 34994761	Sample: Renal

ENVIRONMENTAL MICROBIOLOGY (2021) IF: 5.491

A novel transcription factor negatively regulates antioxidant response, cell wall integrity and virulence in the fungal insect pathogen, Beauveria bassiana

DOI: 10.1111/1462-2920.15397
PMID: 33432709	Sample: Cell Sample

FOOD CHEMISTRY (2021) IF: 7.514

Cytotoxicity of adducts formed between quercetin and methylglyoxal in PC-12 cells

DOI: 10.1016/j.foodchem.2021.129424
PMID: 33706136	Sample: Cell Lysate

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Q&A

C************uSubmitted [ Sep 04 2024 ]

Asked: Dear Elabscience Support Team, I recently purchased the Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit from your company. According to the product instructions, it mentions that Reagent 2, Reagent 3, Reagent 4, Reagent 8, and Reagent 9 should be stored at -20°C, while the others should be stored at 2-8°C. However, the entire product package was delivered to me at 2-8°C. I would like to know if it is acceptable for the reagents, which require different storage temperatures, to be stored in the same box without opening the packaging. Thank you for your assistance. Best regards,

adminSubmitted [ Sep 04 2024 ]

Answered: Dear Cagatay Oltulu, Thanks for your interest in Elabscience products. We generally transport the kit with ice pack within 2 weeks, it can be used normally. The overall temperature of the kit in the short-term transportation process is 2-8℃, which has little impact on the reagent. After receiving the kit, you can store the reagent at the corresponding temperature according to the manual. For any other question, please let us know. Regards,

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