Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K097-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $280
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(412 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Detection principle

      GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.

      Performance characteristics

      Synonyms T-GSH,GSSG
      Sample type Serum,plasma,animal tissue,red blood cells,cultured cells
      Sensitivity 0.12 μmol/L T-GSH
      Detection range 0.12-30 μmol/L T-GSH
      Detection method Colorimetric method
      Assay type Quantitative
      Assay time 180 min
      Precision Average inter-assay CV: 4.7%Average intra-assay CV: 0.9%
      Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
      Other reagents required PBS(0.01 M, pH 7.4), Absolute ethanol
      Storage Reagent 2, Reagent 4, Reagent 8, Reagent 9: -20℃; others: 2-8℃
      Valid period 6 months

      Dilution of sample

      It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-30 μmol/L T-GSH).

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human serum

      1

      Rat plasma

      1

      Mouse serum

      1

      10% Rat heart tissue homogenate

      10

      10% Rat liver tissue homogenate

      60

      10% Rat brain tissue homogenate

      10

      Note: The diluent is reagent 3 working solution.

      Citations

      1. Effects of different doses of Prunus laurocerasus L. leaf extract on oxidative stress, hyperglycaemia and hyperlipidaemia induced by type I diabetes.
        IF: /
        Journal: Indian Journal of Traditional Knowledge(2018)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K022-S E-BC-K031-S E-BC-K097-S E-BC-K238 E-BC-K298-M 
        Sample type: Serum,Tissue homogenate
        Reactivity: Rat
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      MSDS for ELISA ISO 9001

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