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Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit

Cat.No.:E-BC-K097-S
Detection instrument: Spectrophotometer(412 nm)

Detection method: Colorimetric method

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Detection principle

GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.

Performance characteristics

Synonyms	T-GSH,GSSG
Sample type	Serum,plasma,animal tissue,red blood cells,cultured cells
Sensitivity	0.12 μmol/L T-GSH
Detection range	0.12-30 μmol/L T-GSH
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	180 min
Precision	Average inter-assay CV: 4.700%Average intra-assay CV: 0.900%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required	PBS(0.01 M, pH 7.4), Absolute ethanol
Storage	Reagent 2, Reagent 3, Reagent 4, Reagent 8, Reagent 9: -20℃; others: 2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-30 μmol/L T-GSH).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Rat plasma	1
Mouse serum	1
10% Rat heart tissue homogenate	10
10% Rat liver tissue homogenate	60
10% Rat brain tissue homogenate	10

Note: The diluent is reagent 3.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

FOOD CHEMISTRY (2021) IF: 7.514
Cytotoxicity of adducts formed between quercetin and methylglyoxal in PC-12 cells

DOI: 10.1016/j.foodchem.2021.129424

PMID: 33706136
Sample: Cell lysate
Food & Function (2021) IF: 5.396
Supplementation of amino acids and organic acids prevents the increase in blood pressure induced by high salt in Dahl salt-sensitive rats

DOI: 10.1039/D1FO03577K

PMID: 34994761
Sample: renal
ENVIRONMENTAL MICROBIOLOGY (2021) IF: 5.491
A novel transcription factor negatively regulates antioxidant response, cell wall integrity and virulence in the fungal insect pathogen, Beauveria bassiana

DOI: 10.1111/1462-2920.15397

PMID: 33432709
Sample: Cell sample
Bioengineered (2022) IF: 3.269
SNAI2 promotes the development of ovarian cancer through regulating ferroptosis

DOI: 10.1080/21655979.2021.2024319

PMID: 35220872
PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

DOI: 10.1080/13880209.2022.2070221

PMID: 35635784
Sample: brain tissue
Porcine Health Management (2022) IF: 3.535
Is a new generation of mycotoxin clay adsorbents safe in a pig’s diet?

DOI: 10.1186/s40813-022-00275-w

Sample: blood
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2021) IF: 3.639
Effects of almonds on ameliorating salt-induced hypertension in Dahl salt-sensitive rats

DOI: 10.1002/jsfa.11611

PMID: 34708426
Sample: renal cortex

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