Current Position:Home>>Kits>>Biochemical Kits>> Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit
Catalog number:E-BC-K097-M
Detection method: Colorimetric method
Detection instrument: Microplate reader(405-415 nm,optimum wavelength: 412 nm)
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Add to cart Compare Bulk requestSynonyms | T-GSH,GSSG |
Sample type | Serum,plasma,animal tissue,red blood cells,cultured cells |
Sensitivity | 0.36 μmol/L T-GSH |
Detection range | 0.36-30 μmol/L T-GSH |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 100 min |
Precision | Average inter-assay CV: 3.9%Average intra-assay CV: 0.6% |
Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Other reagents required | PBS (0.01 M, pH 7.4), Absolute ethanol |
Storage | -20℃ |
Valid period | 6 months |
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.36-30 μmol/L T-GSH).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human plasma |
1 |
10% Rat liver tissue homogenate |
10-20 |
10% Rat kidney tissue homogenate |
1 |
10% Rat heart tissue homogenate |
10-20 |
10% Mouse brain tissue homogenate |
2-5 |
HepG2 cells |
1 |
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w*****gSubmitted [ Nov 21 2020 ]