Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
    • GSH Standard Curve-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • GSH Standard Curve-Elabscience
    • Biochemical Assay Kit-Elabscience
    • GSH Standard Curve-Elabscience

      Catalog number:E-BC-K097-M

      Size:
      • 96T
      Qty:
      - +
      Price: $280
      Can detect 80 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(405-415 nm,optimum wavelength: 412 nm)

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      Detection principle

      GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.

      Performance characteristics

      Synonyms T-GSH,GSSG
      Sample type Serum,plasma,animal tissue,red blood cells,cultured cells
      Sensitivity 0.36 μmol/L T-GSH
      Detection range 0.36-30 μmol/L T-GSH
      Detection method Colorimetric method
      Assay type Quantitative
      Assay time 100 min
      Precision Average inter-assay CV: 3.9%Average intra-assay CV: 0.6%
      Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
      Other reagents required PBS (0.01 M, pH 7.4), Absolute ethanol
      Storage -20℃
      Valid period 6 months

      Dilution of sample

      It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.36-30 μmol/L T-GSH).

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human plasma

      1

      10% Rat liver tissue homogenate

      10-20

      10% Rat kidney tissue homogenate

      1

      10% Rat heart tissue homogenate

      10-20

      10% Mouse brain tissue homogenate

      2-5

      HepG2 cells

      1

      Note: The diluent is reagent 3 working solution.

      Citations

      1. Role of Nrf2 dysfunction in the pathogenesis of diabetic nephropathy: Therapeutic prospect of epigallocatechin-3-gallate
        IF: 6.17
        Journal: Free Radical Biology and Medicine(2020)
        DOI: 10.1016/j.freeradbiomed.2020.07.037
        PMID: 32768570
        Products: E-BC-K097-M 
        Sample type: Tissue homogenate
        Reactivity: Rat
      2. Physiological sway of aging on oxidative stress and levels of some minerals in diabetes type II of both female and male in Sulaimaniyah city
        Journal: JOURNAL OF EDUCATION AND SCIENCE(2020)
        DOI: 10.33899/edusj.2020.126325.1031
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K097-M 
        Sample type: Serum
        Reactivity: Human
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      Verified Customer

      w*****gSubmitted [ Nov 21 2020 ]

      • Application:Biochemical
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