Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit

GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.

Sample type	Dilution factor
Human plasma	1
10% Rat liver tissue homogenate	10-20
10% Rat kidney tissue homogenate	1
10% Rat heart tissue homogenate	10-20
10% Mouse brain tissue homogenate	2-5
HepG2 cells	1