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Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit

Cat.No.:E-BC-K097-M
Detection instrument: Microplate reader(405-415 nm,optimum wavelength: 412 nm)

Detection method: Colorimetric method

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96T

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Protocols for metabolism assays

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Detection principle

GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.

Performance characteristics

Synonyms	T-GSH,GSSG
Sample type	Serum,plasma,animal tissue,red blood cells,cultured cells
Sensitivity	0.36 μmol/L T-GSH
Detection range	0.36-30 μmol/L T-GSH
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	100 min
Precision	Average inter-assay CV: 3.900%Average intra-assay CV: 0.600%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	PBS (0.01 M, pH 7.4), Absolute ethanol
Storage	-20℃
Valid period	12 months

Images

The ratio of GSH/GSSG was significantly decreased in CuO2 treated groups.

XX Li et al developed a novel nanoreactor activated by tumor microenvironment for chemiluminescence imaging, can be used for tumor resistance. The ratio between total glutathione and oxidized glutathione (GSH/GSSG) in human MCF-7 cell was determined using GSH/GSSG colorimetric assay kit (E-BC-K097-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.36-30 μmol/L T-GSH).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma	1
10% Rat liver tissue homogenate	10-20
10% Rat kidney tissue homogenate	1
10% Rat heart tissue homogenate	10-20
10% Mouse brain tissue homogenate	2-5
HepG2 cells	1

Note: The diluent is reagent 3.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

CHEMICAL ENGINEERING JOURNAL (2022) IF: 16.744
Tumor microenvironment activated nanoreactors for chemiluminescence imaging-guided simultaneous elimination of breast tumors and tumor-resident intracellular pathogens

DOI: 10.1016/j.cej.2022.139939

Sample: MCF-7 cells
Cell Death & Disease (2022) IF: 9.685
UHRF1-mediated ferroptosis promotes pulmonary fibrosis via epigenetic repression of GPX4 and FSP1 genes

DOI: 10.1038/s41419-022-05515-z

Sample: lung tissue,alveolar epithelial Type2 cells (AEC2)
Antioxidants (2022) IF: 7.675
Selenoprotein F Knockout Caused Glucose Metabolism Disorder in Young Mice by Disrupting Redox Homeostasis

DOI: 10.3390/antiox11112105

PMID: 36358477
Sample: liver,serum
Frontiers in Molecular Biosciences (2022) IF: 6.113
Glucose Deprivation Induced by Acarbose and Oncolytic Newcastle Disease Virus Promote Metabolic Oxidative Stress and Cell Death in a Breast Cancer Model

DOI: 10.3389/fmolb.2022.816510

PMID: 35936786
Sample: mammary adenocarcinoma tissue
FREE RADICAL BIOLOGY AND MEDICINE (2020) IF: 6.17
Role of Nrf2 dysfunction in the pathogenesis of diabetic nephropathy: Therapeutic prospect of epigallocatechin-3-gallate

DOI: 10.1016/j.freeradbiomed.2020.07.037

PMID: 32768570
Sample: Tissue homogenate
MOLECULAR NUTRITION & FOOD RESEARCH (2022) IF: 6.575
Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis

DOI: 10.1002/mnfr.202200341

PMID: 36069237
Sample: colon tissue
Reproductive Biology and Endocrinology (2022) IF: 5.211
Betaine attenuate chronic restraint stress-induced changes in testicular damage and oxidative stress in male mice

DOI: 10.1186/s12958-022-00949-8

PMID: 35597951
Sample: testis tissue
Food & Function (2021) IF: 5.396
Supplementation of amino acids and organic acids prevents the increase in blood pressure induced by high salt in Dahl salt-sensitive rats

DOI: 10.1039/D1FO03577K

PMID: 34994761
Sample: renal
INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
HC067047 as a potent TRPV4 inhibitor repairs endotoxemia colonic injury

DOI: 10.1016/j.intimp.2022.109648

Sample: colon
BIOCHIMIE (2022) IF: 4.079
Glucose deprivation using 2-deoxyglucose and acarbose induce metabolic oxidative stress and apoptosis in female mice bearing breast cancer

DOI: 10.1016/j.biochi.2022.01.007

PMID: 35066100
Sample: breast tumor tissue
Bioengineered (2022) IF: 3.269
SNAI2 promotes the development of ovarian cancer through regulating ferroptosis

DOI: 10.1080/21655979.2021.2024319

PMID: 35220872
PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

DOI: 10.1080/13880209.2022.2070221

PMID: 35635784
Sample: brain tissue
Porcine Health Management (2022) IF: 3.535
Is a new generation of mycotoxin clay adsorbents safe in a pig’s diet?

DOI: 10.1186/s40813-022-00275-w

Sample: blood
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2021) IF: 3.639
Effects of almonds on ameliorating salt-induced hypertension in Dahl salt-sensitive rats

DOI: 10.1002/jsfa.11611

PMID: 34708426
Sample: renal cortex
LIFE SCIENCES (2020) IF: 3.647
l-theanine alleviates liver and kidney dysfunction in septic rats induced by cecal ligation and puncture

DOI: 10.1016/j.lfs.2020.117502

PMID: 32142764
Sample: Tissue homogenate
JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
Viphyllin™, a standardized extract from black pepper seeds, mitigates intestinal inflammation, oxidative stress, and anxiety-like behavior in DSS-induced colitis mice

DOI: 10.1111/jfbc.14306

PMID: 35766031
Sample: colon tissue,brain tissue
Journal of Functional Foods (2020) IF: 3.701
Trehalose against UVB-induced skin photoaging by suppressing MMP expression and enhancing procollagen I synthesis in HaCaT cells

DOI: 10.1016/j.jff.2020.104198

Sample: HaCaT cell
NEUROSCIENCE (2023) IF: 3.708
HC067047 Ameliorates Sepsis-associated Encephalopathy by Suppressing Endoplasmic Reticulum Stress and Oxidative Stress-Induced Pyroptosis in the Hippocampi of Mice

DOI: 10.1016/j.neuroscience.2023.02.005

Sample: hippocampi
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020) IF: 2.05
The renoprotective effect of glycyrrhizic acid in insulin-resistant rats exposed to aluminum involves the inhibition of TLR4/NF-κB signaling pathway

DOI: 10.1007/s00210-020-02012-y

PMID: 33165681
Sample: Tissue homogenate
NEUROLOGICAL RESEARCH (2022) IF: 2.529
Investigation of therapeutic effects of calcium dobesilate in cerebral hypoxia/ reperfusion injury in rats

DOI: 10.1080/01616412.2022.2159109

Sample: brains
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED (2022) IF: 2.687
Evaluation of the therapeutic effects of calcium dobesilate in sciatic nerve crush injury in rats

DOI: 10.1016/j.injury.2022.08.061

PMID: 36070970
Sample: sciatic nerve
JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
A standardized black pepper seed extract containing β-caryophyllene improves cognitive function in scopolamine-induced amnesia model mice via regulation of brain-derived neurotrophic factor and MAPK proteins

DOI: 10.1111/jfbc.13994

PMID: 34778972
Sample: brain
Animals (2021) IF: 2.752
The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler Chickens

DOI: 10.3390/ani11020399

PMID: 33557433
Sample: Plasma
International Journal of Inflammation (2020)
Therapeutic Effects of the Combination of Alpha-Lipoic Acid (ALA) and Coenzyme Q10 (CoQ10) on Cisplatin-Induced Nephrotoxicity

DOI: 10.1155/2020/5369797

PMID: 32328233
Sample: Tissue homogenate

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Reviews/Q&A

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Reviews
Q&A

M*****aSubmitted [ Sep 23 2022 ]

Asked: What is GSSG homogenate medium and diluent?

adminSubmitted [ Sep 23 2022 ]

Answered: The homogenate medium and diluent are all reagent 3 working solution.

M*****aSubmitted [ Sep 06 2022 ]

Asked: Human Oxidized Glutathione ELISA Kit, 96 T, (E-BC-K097-M), does BCA need to be used?

adminSubmitted [ Sep 06 2022 ]

Answered: There is no need to use BCA kit, no matter what the sample is.

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