Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K071-S

      Size:
      • 50Assays
      Qty:
      - +
      Price: $180
      Can detect 48 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(520 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      The excess iron is added to the serum to bind all the ferritin in the serum, and the excess iron is adsorbed by adding the iron adsorbent. The iron bind with the ferritin is separated from the protein by the action of acid solution and reductant. Fe3+ in serum is reduced to Fe2+, Fe2+ binds with bipyridine to form pink complex. In a certain range, the amount of TIBC is positively correlated with the depth of color. The iron content measured is, minus serum iron value, which is called unsaturated iron binding force. Total iron binding capacity minus serum iron value is unsaturated iron binding capacity (UIBC).

      Performance characteristics

      Synonyms TIBC
      Sample type serum
      Sensitivity 0.03 mg/L
      Detection range 0.03-50 mg/L
      Precision Average inter-assay CV: 4.7%Average intra-assay CV: 3.4%
      Other instruments required Micropipettor, Water bath, Vortex mixer, Centrifuge
      Other reagents required Normal saline (0.9% NaCl)
      Storage 2-8℃
      Valid period 6 months
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      MSDS for ELISA ISO 9001

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