Total Sulfhydryl Group/Total Thiol (-SH) Colorimetric Assay Kit (E-BC-K265-M)
For research use only.
| Detection Principle | Sulfhydryl compounds react with 5,5' -dithiobis (2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which have a maximum absorption peak at 412 nm. Measure the OD value and calculate the total mercapto content indirectly. |
| Synonyms | T-SH |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (410-420 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Absolute ethyl alcohol,Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 9.91 μmol/L |
| Detection Range | 9.91-1000 μmol/L |
| Precision | inter-assay CV: 2.9% | intra-assay CV: 2.5% |
| Sample Volume | 80 μL |
| Assay Time | 20 min |
Note: Data for this module come from published literature that cites this product, displaying other products used together in the same research paper.
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 4-6 |
| Human plasma | 4-6 |
| Human urine | 1 |
| Rabbit serum | 3-5 |
| Porcine serum | 4-6 |
| 10% Rat liver tissue homogenate | 4-6 |
| 10% Rat kidney tissue homogenate | 4-6 |
| 10% Rat spleen tissue homogenate | 4-6 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
No files available. Please enter the correct identification code in the search area to query the relevant documents.
| Title | Status | Valid until | Action | |
|---|---|---|---|---|
| {{v.文件名}} | {{v.status_str}} | {{v.timestamp_str}} | Download | / |
*Note：Please download the relevant files within the validity period. After expiration, file preview and download will not be available on the page.
