Click here to view cell apoptosis assay products
×

Current Position:Home>>Kits>>Metabolism Assays>> Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

Citations(71)

  • Cat.No.:E-BC-K019-S

  • Detection instrument: Spectrophotometer(550 nm)

Manual MSDS

To Purchase E-BC-K019-S

Size:
  • 100Assays
  • 50Assays
Price: $280
Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

 Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.

Performance characteristics

Synonyms T-SOD
Sample type Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity 4.7 U/mL
Detection range 4.7-166 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 100 min
Precision Average inter-assay CV: 6.300%Average intra-assay CV: 2.800%
Other instruments required Micropipettor, Vortex mixer, Water bath, Centrifuge
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage Reagent 4: -20℃, others: 2-8℃
Valid period 12 months

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

The volume of sample

HepG2 supernatant

1

50 μL

HepG2 cell

8-10

25 μL

Mouse serum

3-5

20 μL

10% Mouse liver tissue homogenate

40-60

20 μL

10% Rat kidney tissue homogenate

15-20

20 μL

Human urine

1

25 μL

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


Citations

  1. Bioactive Materials (2023) IF: 16.874
    Gold nanoparticles-embedded ceria with enhanced antioxidant activities for treating inflammatory bowel disease

    DOI: 10.1016/j.bioactmat.2023.01.015
    Sample: colon tissue
  2. JOURNAL OF HAZARDOUS MATERIALS (2021) IF: 10.588
    Sequential use of UV-LEDs irradiation and chlorine to disinfect waterborne fungal spores: Efficiency, mechanism and photoreactivation

    DOI: 10.1016/j.jhazmat.2021.127102

    PMID: 34482083
  3. CELLULAR & MOLECULAR BIOLOGY LETTERS (2023) IF: 8.702
    Formyl peptide receptor 2 activation by mitochondrial formyl peptides stimulates the neutrophil proinflammatory response via the ERK pathway and exacerbates ischemia–reperfusion injury

    DOI: 10.1186/s11658-023-00416-1
    Sample: Kidney tissue
  4. PLANT CELL AND ENVIRONMENT (2022) IF: 7.228
    The rice heavy-metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homoeostasis

    DOI: 10.1111/pce.14263

    PMID: 35040151

    		 Sample: leaf
  5. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Tetramethylpyrazine Protects Endothelial Injury and Antithrombosis via Antioxidant and Antiapoptosis in HUVECs and Zebrafish

    DOI: 10.1155/2022/2232365

    PMID: 35898617

    		 Sample: HUVECs
  6. Antioxidants (2022) IF: 7.675
    Selenoprotein F Knockout Caused Glucose Metabolism Disorder in Young Mice by Disrupting Redox Homeostasis

    DOI: 10.3390/antiox11112105

    PMID: 36358477

    		 Sample: liver,serum
  7. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Palm Oil Derived Tocotrienol-Rich Fraction Attenuates Vascular Dementia in Type 2 Diabetic Rats

    DOI: 10.3390/ijms232113531
    Sample: brain
  8. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

    DOI: 10.3390/ijms232315400
    Sample: kidney
  9. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 6.291
    Antibacterial effects of nano-decoction iron polysulfide in epididymitis and the systematic evaluation of its toxicity on the reproductive health of male mice

    DOI: 10.1016/j.ecoenv.2022.113184

    PMID: 35032726

    		 Sample: testis
  10. Cell Death & Disease (2020) IF: 6.304
    Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload

    DOI: 10.1038/s41419-020-02777-3
    Sample: heart
  11. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2022) IF: 6.51
    Carboxymethyl cellulose/sericin-based hydrogels with intrinsic antibacterial, antioxidant, and anti-inflammatory properties promote re-epithelization of diabetic wounds in rats

    DOI: 10.1016/j.ijpharm.2022.122328

    PMID: 36280221

    		 Sample: skin tissues
  12. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Lipoic acid (LA) dose-dependently protects bone losses in the mandible of rats during the development of osteopenia by inhibiting oxidative stress and promoting bone formation

    DOI: 10.1016/j.biopha.2021.112467

    PMID: 34891114

    		 Sample: serum
  13. Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
    Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation

    DOI: 10.1155/2022/7655142
    Sample: nucleus pulposus(NP) cell
  14. LIFE SCIENCES (2022) IF: 6.78
    A bovine milk-derived peptide ameliorates alloxan-injured pancreatic β cells through IRS2/PI3K/Akt signaling

    DOI: 10.1016/j.lfs.2022.120907

    PMID: 36037870

    		 Sample: serum
  15. Antioxidants (2020) IF: 5.014
    Dietary Postbiotic Lactobacillus plantarum Improves Serum and Ruminal Antioxidant Activity and Upregulates Hepatic Antioxidant Enzymes and Ruminal Barrier Function in Post-Weaning Lambs

    DOI: 10.3390/antiox9030250
    Sample: serum,ruminal fluid
  16. LIFE SCIENCES (2021) IF: 5.037
    Protective effects of low-temperature plasma on cisplatin-induced nephrotoxicity

    DOI: 10.1016/j.lfs.2021.120230

    PMID: 34919900
  17. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079
    Sample: livers
  18. Food & Function (2021) IF: 5.396
    Alhagi honey polysaccharides attenuate intestinal injury and immune suppression in cyclophosphamide-induced mice

    DOI: 10.1039/D1FO01008E

    PMID: 34128029

    		 Sample: small intestinal
  19. Journal of Environmental Sciences (2022) IF: 5.565
    Alleviative effects of a novel strain Bacillus coagulans XY2 on copper-induced toxicity in zebrafish larvae

    DOI: 10.1016/j.jes.2022.02.010

    PMID: 36375957

    		 Sample: larvae
  20. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    The role of AMPK-Sirt1-autophagy pathway in the intestinal protection process by propofol against regional ischemia/reperfusion injury in rats

    DOI: 10.1016/j.intimp.2022.109114

    PMID: 35933747

    		 Sample: intestinal tissue,serum
  21. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY (2022) IF: 5.79
    Mitigation of benznidazole toxicity and oxidative stress following ascorbic acid supplementation in an adult traveller with chronic indeterminate Chagas’ disease

    DOI: 10.1093/jac/dkac093
    Sample: serum
  22. LIVER TRANSPLANTATION (2022) IF: 5.799
    Cerium oxide nanoparticles administration during machine perfusion of discarded human livers: a pilot study

    DOI: 10.1002/lt.26421

    PMID: 35100468

    		 Sample: liver
  23. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
    Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

    DOI: 10.1016/j.sjbs.2022.103495
    Sample: testicular tissue
  24. BIOLOGICAL TRACE ELEMENT RESEARCH (2022) IF: 4.081
    Selenium Supplementation Protects Against Arsenic-Trioxide-Induced Cardiotoxicity Via Reducing Oxidative Stress and Inflammation Through Increasing NAD+ Pool

    DOI: 10.1007/s12011-022-03478-y
    Sample: heart tissue
  25. ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.109
    hUC-MSCs exosomal miR-451 alleviated acute lung injury by modulating macrophage M2 polarization via regulating MIF-PI3K-AKT signaling pathway

    DOI: 10.1002/tox.23639
    Sample: serum,NR8383 cell
  26. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Prenatal exposure to environmental pro-oxidants induces mitochondria-mediated epigenetic changes: a cross-sectional pilot study

    DOI: 10.1007/s11356-022-21059-3

    PMID: 35633452

    		 Sample: plasma
  27. Journal of Diabetes Investigation (2021) IF: 4.232
    Diet-induced prediabetes: Effects on the activity of the renin-angiotensin-aldosterone system (RAAS) in selected organs

    DOI: 10.1111/jdi.13690
    Sample: skeletal muscle,heart,pancreas
  28. Foods (2022) IF: 4.35
    Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota

    DOI: 10.3390/foods11111551
    Sample: colon tissue
  29. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    In vitro antiaging activity of polyphenol rich Polyalthia longifolia (Annonaceae) leaf extract in Saccharomyces cerevisiae BY611 yeast cells

    DOI: 10.1016/j.jep.2022.115110

    PMID: 35181488
  30. International Journal of Nanomedicine (2018) IF: 4.37
    Tanshinol borneol ester on nanostructured lipid carriers has longer brain and systemic effector retention and better antioxidant activity in vivo

    DOI: 10.2147/IJN.S159789

    PMID: 29695905

    		 Sample: Tissue homogenate
  31. Scientific Reports (2022) IF: 4.38
    Molecular mechanisms associated with microbial biostimulant-mediated growth enhancement, priming and drought stress tolerance in maize plants

    DOI: 10.1038/s41598-022-14570-7

    PMID: 35729338

    		 Sample: leaf
  32. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY (2022) IF: 4.553
    Investigation of the protective effect of gel incorporating Eugenia jambolana leaf extract on 5-fluorouracil-induced oral mucositis: an animal study

    DOI: 10.1007/s00432-022-04065-3

    PMID: 35622166

    		 Sample: cheek,liver,kidney
  33. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Vernodalin regulated the NF-κβp65 signaling in inflammation of lipopolysaccharide -induced sepsis rats

    DOI: 10.1016/j.procbio.2022.10.004
    Sample: liver,kidney,lungs
  34. MOLECULES (2022) IF: 4.927
    Chemical Profile of Cyperus laevigatus and Its Protective Effects against Thioacetamide-Induced Hepatorenal Toxicity in Rats

    DOI: 10.3390/molecules27196470

    PMID: 36235007

    		 Sample: plasma
  35. IMMUNOBIOLOGY (2022) IF: 3.144
    PM2.5 induced lung injury through upregulating ROS-Dependent NLRP3 Inflammasome-Mediated Pyroptosis

    DOI: 10.1016/j.imbio.2022.152207

    PMID: 35344845

    		 Sample: RAW264.7 cell
  36. Animals (2023) IF: 3.231
    The Effects of Dietary Spirulina platensisis on Physiological Responses of Broiler Chickens Exposed to Endotoxin Stress

    DOI: 10.3390/ani13030363
    Sample: Plasma
  37. Environmental Research Communications (2022) IF: 3.237
    Metal accumulation in muscle and oxidative stress response in the liver of juvenile Oreochromis niloticus from contaminated sediment under a simulation of increasing temperature

    DOI: 10.1088/2515-7620/ac803d
    Sample: liver
  38. Biomed Research International (2022) IF: 3.246
    Red Raspberry Extract Decreases Depression-Like Behavior in Rats by Modulating Neuroinflammation and Oxidative Stress

    DOI: 10.1155/2022/9943598

    PMID: 35818443

    		 Sample: hippocampal tissue
  39. Bioengineered (2022) IF: 3.269
    Methylprednisolone up-regulates annexin A1 (ANXA1) to inhibit the inflammation, apoptosis and oxidative stress of cigarette smoke extract (CSE)-induced bronchial epithelial cells, a chronic obstructive pulmonary disease in vitro model, through the formyl

    DOI: 10.1080/21655979.2022.2031769
    Sample: BEAS-2B cells
  40. ANIMAL FEED SCIENCE AND TECHNOLOGY (2022) IF: 3.313
    Housefly maggot meal complement soybean meal in a fish-free diet for hybrid catfish (Clarias gariepinus ♀ x Heterobranchus longifilis ♂): Effect on growth, body composition, blood biochemistry and antioxidant enzyme activity

    DOI: 10.1016/j.anifeedsci.2022.115543
    Sample: serum
  41. Processes (2022) IF: 3.352
    Gastroprotective, Biochemical, and Acute Toxicity Effects of Papaver decaisnei against Ethanol-Induced Gastric Ulcers in Rats

    DOI: 10.3390/pr10101985
    Sample: gastric tissue
  42. Processes (2022) IF: 3.352
    Gastroprophylactic Effects of p-Cymene in Ethanol-Induced Gastric Ulcer in Rats

    DOI: 10.3390/pr10071314
    Sample: stomach
  43. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY (2019) IF: 3.377
    -arginine reverses aging process through suppression of oxidative stress, inflammation, and apoptosis in the rat heart

    DOI: 10.1007/s00424-019-02311-1
    Sample: Tissue homogenate
  44. International Journal of Environmental Research and Public Health (2021) IF: 3.39
    Polystyrene Microplastics Exposure: An Insight into Multiple Organ Histological Alterations, Oxidative Stress and Neurotoxicity in Javanese Medaka Fish (Oryzias javanicus Bleeker, 1854)

    DOI: 10.3390/ijerph18189449
    Sample: intestine,brain
  45. PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
    Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    		 Sample: brain tissue
  46. METABOLIC BRAIN DISEASE (2022) IF: 3.584
    LncRNA ZFAS1 regulates the hippocampal neurons injury in epilepsy through the miR-15a-5p/OXSR1/NF-κB pathway

    DOI: 10.1007/s11011-022-01013-5

    PMID: 35751788

    		 Sample: hippocampal neurons
  47. LIFE SCIENCES (2020) IF: 3.647
    Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

    DOI: 10.1016/j.lfs.2020.117584

    PMID: 32220623

    		 Sample: Tissue homogenate
  48. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Astragaloside IV alleviates PM2.5-induced lung injury in rats by modulating TLR4/MyD88/NF-κB signalling pathway

    DOI: 10.1016/j.intimp.2020.107290

    PMID: 33383446

    		 Sample: lung tissue
  49. CELLULAR SIGNALLING (2020) IF: 3.968
    Circular RNA circ_DROSHA alleviates the neural damage in a cell model of temporal lobe epilepsy through regulating miR-106b-5p/MEF2C axis

    DOI: 10.1016/j.cellsig.2020.109901

    PMID: 33370579

    		 Sample: SK-N-SH cell,IMR-32 cell
  50. International Journal of General Medicine (2022) IF: 2.145
    Detection of Vascular Inflammation and Oxidative Stress by Cotinine in Smokers: Measured Through Interleukin-6 and Superoxide Dismutase

    DOI: 10.2147/IJGM.S367125
    Sample: serum
  51. Translational Andrology and Urology (2022) IF: 2.479
    Astragaloside IV protects against ischemia/reperfusion (I/R)-induced kidney injury based on the Keap1-Nrf2/ARE signaling pathway.

    DOI: 10.21037/tau-22-505
    Sample: kidney tissue
  52. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Zanthoxylum bungeanum Seed Oil Attenuates LPS-Induced BEAS-2B Cell Activation and Inflammation by Inhibiting the TLR4/MyD88/NF-κB Signaling Pathway

    DOI: 10.1155/2021/2073296
    Sample: BEAS-2B cell
  53. Sleep and Breathing (2023) IF: 2.655
    The role of ferroptosis in chronic intermittent hypoxia-induced cognitive impairment

    DOI: 10.1007/s11325-022-02760-6
    Sample: hippocampal tissue
  54. Journal of Stroke & Cerebrovascular Diseases (2022) IF: 2.677
    Circ_0101874 overexpression strengthens PDE4D expression by targeting miR-335-5p to promote neuronal injury in ischemic stroke

    DOI: 10.1016/j.jstrokecerebrovasdis.2022.106817

    PMID: 36252429

    		 Sample: SK-N-SH cells
  55. Toxicology Research (2022) IF: 2.68
    Glutathione attenuated lambda-cyhalothrin-induced alteration of serum total cholesterol concentration and oxidative stress parameters in rats

    DOI: 10.1093/toxres/tfac080
    Sample: serum
  56. EXPERIMENTAL BIOLOGY AND MEDICINE (2021) IF: 2.691
    Fetuin-A exerts a protective effect against experimentally induced intestinal ischemia/reperfusion by suppressing autophagic cell death:

    DOI: 10.1177/1535370221995207
    Sample: Tissue homogenate(Jejunum)
  57. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72
    Watermelon (Citrullus lanatus) leaf extract attenuates biochemical and histological parameters in high-fat diet/streptozotocin-induced diabetic rats

    DOI: 10.1111/jfbc.14058

    PMID: 34981526

    		 Sample: serum
  58. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72
    Effect of coconut water and milk on heat stress-induced gastrointestinal tract dysmotility in rats: Role of oxidative stress and inflammatory response

    DOI: 10.1111/jfbc.14129

    PMID: 35298033

    		 Sample: gastric,jejunal, colonic
  59. ANDROLOGIA (2021) IF: 2.775
    Achillea millefolium alleviates testicular damage in paclitaxel-intoxicated rats via attenuation of testicular oxido-inflammatory stress and apoptotic responses

    DOI: 10.1111/and.14028

    PMID: 33650701

    		 Sample: Tissue homogenate(testicular)
  60. ECOTOXICOLOGY (2021) IF: 2.823
    Garlic (Allium sativum) and Fu-ling (Poria cocos) mitigate lead toxicity by improving antioxidant defense mechanisms and chelating ability in the liver of grass carp (Ctenopharyngodon idella)

    DOI: 10.1007/s10646-021-02405-6

    PMID: 33830385

    		 Sample: Tissue homogenate
  61. Journal of Diabetes Research (2018) IF: 2.885
    The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers

    DOI: 10.1155/2018/7341242
    Sample: kidney tissues
  62. CURRENT ISSUES IN MOLECULAR BIOLOGY (2023) IF: 2.976
    Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling Pathways

    DOI: 10.3390/cimb45020057
    Sample: colonic tissue
  63. BIOLOGICAL RHYTHM RESEARCH (2022) IF: 1.219
    Impact of photoperiods on the specific activities of immune and antioxidant enzymes in different tissues of Dybowski’s frog (Rana dybowskii)

    DOI: 10.1080/09291016.2022.2043588
    Sample: serum,liver,skin
  64. Experimental and Therapeutic Medicine (2018) IF: 1.41
    Rutin inhibits coronary heart disease through ERK1/2 and Akt signaling in a porcine model

    DOI: 10.3892/etm.2017.5365

    PMID: 29250160

    		 Sample: Urine
  65. WORK-A Journal of Prevention Assessment & Rehabilitation (2021) IF: 1.505
    Evaluation of oxidative stress and pro-inflammatory cytokines in occupationally cadmium exposed workers

    DOI: 10.3233/WOR-203302

    PMID: 33104053

    		 Sample: serum
  66. BRAZILIAN JOURNAL OF BIOLOGY (2023) IF: 1.651
    Neuroprotective potential of lignan-rich fraction of Piper cubeba L. by improving antioxidant capacity in the rat's brain

    DOI: 10.1590/1519-6984.266573
    Sample: brain
  67. Journal of Plant Interactions (2018) IF: 1.655
    Brevibacillus sp. promotes maize root colonization by Acaulospora tuberculata and the alteration of associated plant protein responses

    DOI: 10.1080/17429145.2018.1547844
    Sample: maize roots
  68. Molecular Medicine Reports (2018) IF: 1.922
    Isopsoralen regulates PPAR‑γ/WNT to inhibit oxidative stress in osteoporosis

    DOI: 10.3892/mmr.2017.7954
    Sample: Serum
  69. ENVIRONMENTAL MONITORING AND ASSESSMENT (2019) IF: 1.959
    Biochemical investigation of association of arsenic exposure with risk factors of diabetes mellitus in Pakistani population and its validation in animal model

    DOI: 10.1007/s10661-019-7670-2

    PMID: 31346790

    		 Sample: Blood,Urine
  70. Journal of Toxicology (2020)
    Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts

    DOI: 10.1155/2020/1807126
    Sample: C2C12 cell
  71. Advances in Traditional Medicine (2020)
    Anti-hypernociceptive effects of methanol extract of Boswellia dalzielii on STZ-induced diabetic neuropathic pain

    DOI: 10.1007/s13596-019-00411-y
    Sample: serum,sciatic nerves
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A

Verified Customer

M*sSubmitted [ Jun 12 2019 ]

  • Application:Biochemical
  • Description:The T-SOD activity in HepG2 cell supernatant was detected by the SOD kit, By asking the loading quantity of technical support samples, they gave a reference loading quantity and the loading amount of the formal experiment is similar to that of the reference. At this point, it\'s a trusty assay kit
Read More
Read Less

Verified Customer

M**kSubmitted [ May 29 2019 ]

  • Application:Biochemical
  • Description:The instructions tell us the treatment method of samples in detail,But he preparation part of this biochemical Assay kit is a little too much.
Read More
Read Less

Verified Customer

r****eSubmitted [ May 29 2019 ]

  • Application:Biochemical
  • Description:I have consulted many literatures and know that the results of SOD activity detection by different methods will be different, so it is more difficult to find the literature of SOD activity range. I think the best part of this kit is the ‘Reference values for samples’ section in the instruction, which gives the reference range of SOD activity of various samples. Using this kit (E-BC-K019) to detect human plasma samples, the results are within the reference range provided by the kit, which saves me a lot of work.
Read More
Read Less
Q

j***sSubmitted [ Oct 17 2023 ]

Asked: It\'s not written how much sample should I use while starting the assay.

Reply

A

adminSubmitted [ Oct 17 2023 ]

Answered: The SOD activity of different samples is different, and the optimal sampling volume of different species is also different, and the optimal sampling volume should be determined according to the calculation result of the inhibition rate. It is recommended to take 2~3 samples before the formal experiment for pre-experiment, and calculate the inhibition rate according to the manual to determine the optimal sampling volume.

Q

M*****iSubmitted [ Oct 09 2022 ]

Asked: Can I still use it on samples that have been treated with RIPA?

Reply

A

adminSubmitted [ Oct 09 2022 ]

Answered: It is usually not recommended to treat the sample with lysate. If the sample is already prepared, you'd better make a pre-test first.

Q

M*****iSubmitted [ Aug 17 2022 ]

Asked: What is the recommended dilution factor of mouse serum?

Reply

A

adminSubmitted [ Aug 17 2022 ]

Answered: The reference dilution for mouse serum is 3-5 times.

... Show All Show Less

People Also Bought

About Us

Company Information

Distributors

Contact us

News

Return and Refund Policy

dhl Fedex
Terms and Conditions | Privacy Policy | Cookie Policy  ©2009-2024Elabscience Bionovation Inc. All Rights Reserved.
鄂ICP备18003807号-3
We use cookies to improve your browsing experience, serve personalized ads or content, and for analytics and metrics about our visitors both on this website and other media. By clicking "Accept All", you consent to our use of cookies. To find out more about the cookies we use, see our Cookie Policy
Accept All Reject All
Apply for FREE TRIAL Fluorometric Assay kit
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha:
Click to load Captcha