Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method) (E-BC-K019-S)
For research use only.
| Detection Principle | The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula. |
| Detection Principle Image | |
| Synonyms | T-SOD |
| Sample Type | Serum,Plasma,Urine,Cell,Cell culture supernatant,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (550 nm) |
| Research Area | Oxidative Stress , Plant Physiology Series |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 4.7 U/mL |
| Detection Range | 4.7-166 U/mL |
| Precision | inter-assay CV: 6.3% | intra-assay CV: 2.8% |
| Sample Volume | 20-50 μL |
| Assay Time | 1 h 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| HepG2 supernatant | 1 |
| HepG2 cell | 8-10 |
| Mouse serum | 3-5 |
| 10% Mouse liver tissue homogenate | 40-60 |
| 10% Rat kidney tissue homogenate | 15-20 |
| Human urine | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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