Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

    • Biochemical Assay Kit-Elabscience
    <
    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K019-M

      Size:
      • 96T
      • 48T
      Qty:
      - +
      Price: $280
      Can detect 92 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(540-560 nm,optimum wavelength: 550 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.

      Performance characteristics

      Synonyms T-SOD
      Sample type serum,plasma,urine,cells,cell culture supernatant,tissue
      Sensitivity 2.4 U/mL
      Detection range 2.4-61 U/mL
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 70 min
      Precision Average inter-assay CV: 5.6%Average intra-assay CV: 5.5%
      Other instruments required Micropipettor, Centrifuge, Incubator
      Other reagents required PBS (0.01 M, pH 7.4)
      Storage Reagent 4: -20℃, others: 2-8℃
      Valid period 6 months

      Dilution of sample

      The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 10%~50% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

      submit review
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA ISO 9001

      Browsing History


        People Also Bought

        Apply for Free Shipping 24T ELISA Kit
        *Product Name:
        *Catalog Number:
        Size:
        *Name:
        *Email:
        *Phone:
        *Message:
        *Country:
        How do you know
        Elabscience:

        *Verification code: