Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K019-M

      • 96T
      • 48T
      - +
      Price: $280
      Can detect 92 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(540-560 nm,optimum wavelength: 550 nm)

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      Detection principle

      The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.

      Performance characteristics

      Synonyms T-SOD
      Sample type Serum,plasma,urine,cells,cell culture supernatant,tissue
      Sensitivity 2.4 U/mL
      Detection range 2.4-61 U/mL
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 70 min
      Precision Average inter-assay CV: 5.6%Average intra-assay CV: 5.5%
      Other instruments required Micropipettor, Centrifuge, Incubator
      Other reagents required PBS (0.01 M, pH 7.4), Normal saline (0.9% NaCl)
      Storage Reagent 4: -20℃, others: 2-8℃
      Valid period 6 months

      Dilution of sample

      The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 10%~50% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human serum


      Rat serum


      Mouse serum


      HepG2 cell


      Human urine


      10% Mouse liver tissue homogenate


      10% Epipremnum aureumtissue homogenate


      10% Mouse brain tissue homogenate


      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

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