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Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

  • Cat.No.:E-BC-K019-M

  • Detection instrument: Microplate reader(540-560 nm,optimum wavelength: 550 nm)

To Purchase E-BC-K019-M

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  • 48T
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Protocols for metabolism assays

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Detection principle

The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.

Performance characteristics

Synonyms T-SOD
Sample type Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity 2.4 U/mL
Detection range 2.4-61 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 70 min
Precision Average inter-assay CV: 5.600%Average intra-assay CV: 5.500%
Other instruments required Micropipettor, Centrifuge, Incubator
Other reagents required PBS (0.01 M, pH 7.4), Normal saline (0.9% NaCl)
Storage Reagent 4: -20℃, others: 2-8℃
Valid period 12 months

Images

The activity of SOD was significantly reduced in advanced atherosclerosis (aAT) group. (p<0.0001)

M K Tembhre et al investigates the function of interleukin-33 in advanced atherosclerosis. Total superoxide dismutase (T-SOD) activity of human PBMCs was determined using T-SOD activity assay kit (E-BC-K019-M).

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 10%~50% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):


Sample type

Dilution factor

Human serum

2-4

Rat serum

4-6

Mouse serum

4-6

HepG2 cell

15-30

Human urine

2-5

10% Mouse liver tissue homogenate

160-200

10% Epipremnum aureumtissue homogenate

3-5

10% Mouse brain tissue homogenate

80-100



Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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  3. CELLULAR & MOLECULAR BIOLOGY LETTERS (2023) IF: 8.702
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  4. PLANT CELL AND ENVIRONMENT (2022) IF: 7.228
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  6. Antioxidants (2022) IF: 7.675
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  7. Antioxidants (2022) IF: 7.675
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  8. Antioxidants (2022) IF: 7.675
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    Sample: peripheral blood mononuclear cells (PBMCs)
  9. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
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    Sample: brain
  10. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
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  11. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 6.291
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  12. Cell Death & Disease (2020) IF: 6.304
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    Sample: heart
  13. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2022) IF: 6.51
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    DOI: 10.1016/j.ijpharm.2022.122328

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  14. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
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    DOI: 10.1016/j.biopha.2021.111570

    PMID: 33932738

    Sample: Tissue homogenate(kidney)
  15. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
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    DOI: 10.1016/j.biopha.2021.112467

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    Sample: serum
  16. Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
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    DOI: 10.1155/2022/7655142

    Sample: nucleus pulposus(NP) cell
  17. Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
    Leonurine Ameliorates Oxidative Stress and Insufficient Angiogenesis by Regulating the PI3K/Akt-eNOS Signaling Pathway in H2O2-Induced HUVECs

    DOI: 10.1155/2021/9919466

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  18. LIFE SCIENCES (2022) IF: 6.78
    A bovine milk-derived peptide ameliorates alloxan-injured pancreatic β cells through IRS2/PI3K/Akt signaling

    DOI: 10.1016/j.lfs.2022.120907

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  19. Antioxidants (2020) IF: 5.014
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    DOI: 10.3390/antiox9030250

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  20. Marine Drugs (2021) IF: 5.118
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    Sample: HaCaT cells
  21. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021) IF: 5.31
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    DOI: 10.1111/jcmm.16560

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  22. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021) IF: 5.31
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    DOI: 10.1111/jcmm.16439

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    Sample: Cell culture supernatant
  23. Food Bioscience (2022) IF: 5.318
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    DOI: 10.1016/j.fbio.2022.102079

    Sample: livers
  24. Food & Function (2021) IF: 5.396
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    Sample: small intestinal
  25. Journal of Environmental Sciences (2022) IF: 5.565
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    DOI: 10.1016/j.jes.2022.02.010

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    Sample: larvae
  26. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
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    DOI: 10.1016/j.intimp.2022.109114

    PMID: 35933747

    Sample: intestinal tissue,serum
  27. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY (2022) IF: 5.79
    Mitigation of benznidazole toxicity and oxidative stress following ascorbic acid supplementation in an adult traveller with chronic indeterminate Chagas’ disease

    DOI: 10.1093/jac/dkac093

    Sample: serum
  28. Frontiers in Pharmacology (2021) IF: 5.811
    Identification of the Antithrombotic Mechanism of Leonurine in Adrenalin Hydrochloride-Induced Thrombosis in Zebrafish via Regulating Oxidative Stress and Coagulation Cascade.

    DOI: 10.3389/fphar.2021.742954

  29. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
    Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

    DOI: 10.1016/j.sjbs.2022.103495

    Sample: testicular tissue
  30. BIOLOGICAL TRACE ELEMENT RESEARCH (2022) IF: 4.081
    Selenium Supplementation Protects Against Arsenic-Trioxide-Induced Cardiotoxicity Via Reducing Oxidative Stress and Inflammation Through Increasing NAD+ Pool

    DOI: 10.1007/s12011-022-03478-y

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  31. ENVIRONMENTAL TOXICOLOGY (2021) IF: 4.119
    Compound essential oils relieve oxidative stress caused by PM2.5 exposure by inhibiting autophagy through the AMPK/mTOR pathway

    DOI: 10.1002/tox.23297

    Sample: Cell culture supernatant
  32. Drug Design Development and Therapy (2021) IF: 4.162
    Role of Oxidative Stress and Reduced Endogenous Hydrogen Sulfide in Diabetic Nephropathy

    DOI: 10.2147/DDDT.S291591

    PMID: 33707940

    Sample: Tissue homogenate
  33. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Prenatal exposure to environmental pro-oxidants induces mitochondria-mediated epigenetic changes: a cross-sectional pilot study

    DOI: 10.1007/s11356-022-21059-3

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    Sample: plasma
  34. Journal of Diabetes Investigation (2021) IF: 4.232
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    DOI: 10.1111/jdi.13690

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  35. Foods (2022) IF: 4.35
    Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota

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    DOI: 10.1016/j.jep.2022.115110

    PMID: 35181488

  37. Scientific Reports (2022) IF: 4.38
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    DOI: 10.1038/s41598-022-14570-7

    PMID: 35729338

    Sample: leaf
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    Investigation of the protective effect of gel incorporating Eugenia jambolana leaf extract on 5-fluorouracil-induced oral mucositis: an animal study

    DOI: 10.1007/s00432-022-04065-3

    PMID: 35622166

    Sample: cheek,liver,kidney
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    Sample: liver,kidney,lungs
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    PMID: 36235007

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  41. IMMUNOBIOLOGY (2022) IF: 3.144
    PM2.5 induced lung injury through upregulating ROS-Dependent NLRP3 Inflammasome-Mediated Pyroptosis

    DOI: 10.1016/j.imbio.2022.152207

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  42. Animals (2023) IF: 3.231
    The Effects of Dietary Spirulina platensisis on Physiological Responses of Broiler Chickens Exposed to Endotoxin Stress

    DOI: 10.3390/ani13030363

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  43. Biomed Research International (2022) IF: 3.246
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    Sample: hippocampal tissue
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    Sample: BEAS-2B cells
  45. ANIMAL FEED SCIENCE AND TECHNOLOGY (2022) IF: 3.313
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    DOI: 10.1016/j.anifeedsci.2022.115543

    Sample: serum
  46. Processes (2022) IF: 3.352
    Gastroprotective, Biochemical, and Acute Toxicity Effects of Papaver decaisnei against Ethanol-Induced Gastric Ulcers in Rats

    DOI: 10.3390/pr10101985

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    DOI: 10.3390/pr10071314

    Sample: stomach
  48. International Journal of Environmental Research and Public Health (2021) IF: 3.39
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    DOI: 10.3390/ijerph18189449

    Sample: intestine,brain
  49. PHARMACEUTICAL BIOLOGY (2021) IF: 3.503
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    Sample: serum
  50. PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
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    DOI: 10.1080/13880209.2022.2070221

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    Sample: brain tissue
  51. METABOLIC BRAIN DISEASE (2022) IF: 3.584
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    DOI: 10.1007/s11011-022-01013-5

    PMID: 35751788

    Sample: hippocampal neurons
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    DOI: 10.1016/j.intimp.2020.107290

    PMID: 33383446

    Sample: lung tissue
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    DOI: 10.1016/j.cellsig.2020.109901

    PMID: 33370579

    Sample: SK-N-SH cell,IMR-32 cell
  54. PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS (2021) IF: 2.391
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    DOI: 10.1007/s12298-021-00926-2

    Sample: Plant Tissue
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    DOI: 10.1007/s10534-020-00270-0

    Sample: Tissue homogenate(Heart)
  56. Translational Andrology and Urology (2022) IF: 2.479
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    DOI: 10.21037/tau-22-505

    Sample: kidney tissue
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    Zanthoxylum bungeanum Seed Oil Attenuates LPS-Induced BEAS-2B Cell Activation and Inflammation by Inhibiting the TLR4/MyD88/NF-κB Signaling Pathway

    DOI: 10.1155/2021/2073296

    Sample: BEAS-2B cell
  58. Sleep and Breathing (2023) IF: 2.655
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    DOI: 10.1007/s11325-022-02760-6

    Sample: hippocampal tissue
  59. Journal of Stroke & Cerebrovascular Diseases (2022) IF: 2.677
    Circ_0101874 overexpression strengthens PDE4D expression by targeting miR-335-5p to promote neuronal injury in ischemic stroke

    DOI: 10.1016/j.jstrokecerebrovasdis.2022.106817

    PMID: 36252429

    Sample: SK-N-SH cells
  60. Toxicology Research (2022) IF: 2.68
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    DOI: 10.1093/toxres/tfac080

    Sample: serum
  61. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72
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    DOI: 10.1111/jfbc.14058

    PMID: 34981526

    Sample: serum
  62. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72
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    DOI: 10.1111/jfbc.14129

    PMID: 35298033

    Sample: gastric,jejunal, colonic
  63. Journal of Diabetes Research (2018) IF: 2.885
    The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers

    DOI: 10.1155/2018/7341242

    Sample: kidney tissues
  64. CURRENT ISSUES IN MOLECULAR BIOLOGY (2023) IF: 2.976
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    DOI: 10.3390/cimb45020057

    Sample: colonic tissue
  65. PeerJ (2021) IF: 2.984
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    DOI: 10.7717/peerj.11888

    PMID: 34434650

    Sample: seedling
  66. BIOLOGICAL RHYTHM RESEARCH (2022) IF: 1.219
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    DOI: 10.1080/09291016.2022.2043588

    Sample: serum,liver,skin
  67. WORK-A Journal of Prevention Assessment & Rehabilitation (2021) IF: 1.505
    Evaluation of oxidative stress and pro-inflammatory cytokines in occupationally cadmium exposed workers

    DOI: 10.3233/WOR-203302

    PMID: 33104053

    Sample: serum
  68. BRAZILIAN JOURNAL OF BIOLOGY (2023) IF: 1.651
    Neuroprotective potential of lignan-rich fraction of Piper cubeba L. by improving antioxidant capacity in the rat's brain

    DOI: 10.1590/1519-6984.266573

    Sample: brain
  69. Journal of Plant Interactions (2018) IF: 1.655
    Brevibacillus sp. promotes maize root colonization by Acaulospora tuberculata and the alteration of associated plant protein responses

    DOI: 10.1080/17429145.2018.1547844

    Sample: maize roots
  70. Journal of Toxicology (2020)
    Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts

    DOI: 10.1155/2020/1807126

    Sample: C2C12 cell
  71. Toxicology Reports (2019)
    Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

    DOI: 10.1016/j.toxrep.2019.06.009

    Sample: plasma
  72. Advances in Traditional Medicine (2020)
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    DOI: 10.1007/s13596-019-00411-y

    Sample: serum,sciatic nerves
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Reviews/Q&A

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Q

N************RSubmitted [ Aug 05 2023 ]

Asked: Is this kit appropriate for studying with plants (lichens)?

Reply

A

adminSubmitted [ Aug 05 2023 ]

Answered: We'll reply to you by email. Pls pay attention to the mailbox

Q

M*****iSubmitted [ Oct 18 2022 ]

Asked: How to choose E-BC-K020-M and E-BC-K019-M?

Reply

A

adminSubmitted [ Oct 18 2022 ]

Answered: The detection principle of the two kits is different, and you can choose according to the method you need.

Q

M*****iSubmitted [ Sep 08 2022 ]

Asked: What are the sample requirements for this kit?

Reply

A

adminSubmitted [ Sep 08 2022 ]

Answered: Samples should not contain decontamination agents such as SDS, Tween-20, NP-40, Triton X-100, nor reductive reagents such as DTT, 2- mercaptoethanol.

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