CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method) (E-BC-K022-M)
For research use only.
| Detection Principle | Superoxide anion (O2-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not. |
| Detection Principle Image | |
| Synonyms | CuZn-SOD;Mn-SOD;T-SOD |
| Sample Type | Serum,Plasma,Urine,Cell,Cell culture supernatant,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-570 nm, optimum wavelength: 550 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 1.35 U/mL |
| Detection Range | 1.35-62 U/mL |
| Precision | inter-assay CV: 9.6% | intra-assay CV: 5.1% |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Mouse serum | 5-10 |
| Rat serum | 6-15 |
| Urine | 2-3 |
| Human hydrothorax | 2-3 |
| 10% Mouse liver tissue homogenate | 100-200 |
| 10% Mouse brain tissue homogenate | 20-30 |
| 10% Mouse kidney tissue homogenate | 50-120 |
| 10% Rat kidney tissue homogenate | 50-120 |
| HepG2 cells (5.21mgprot/mL) | 15-25 |
| Cell culture supernatant | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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