Transmembrane Buffer (10 ×)

    • Transmembrane Buffer (10×)-Elabscience
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    • Transmembrane Buffer (10×)-Elabscience
    • Transmembrane Buffer (10×)-Elabscience

      Catalog number:E-BC-R333

      Size:
      • 100 mL
      • 200 mL
      • 500 mL
      Qty:
      - +
      Price: $10

      Application: WB

      Storage: 4°C, 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Introduction

      Tris-Glycine transmembrane buffer is used in the wet and semi-dry test of Western blotting experiments. The protein can be transferred from the gel to the PVDF membrane or the NC membrane. This product is supplied with 10 × concentrate for easy transportation (withoutmethanol).

       

      Components

       

      Products

      100 mL

      200 mL

      500 mL

      Transmembrane Buffer (10 ×)

      100 mL

      2 × 100 mL

      2 × 250 mL

      Manual

      1 copy


      Instructions

      This product is a 10 × concentrate, dilute with pure water and methanol to 1 × working solution before use. 

      For example:take 100 mL Transmembrane Buffer (10 ×), 200 mL methanol,dilute with pure water to 1 L.

      In general, 20% methanol is recommended and can be adjusted according to specific situations. If high molecular weight protein transfer is not easy, please adjust the concentration of methanol. For proteins with a molecular weight larger than 200 kDa, the methanol concentration can be reduced from 20% to 5%, and the transmembrane time can be appropriately increased to 3 h.

       

      Self-Prepared Reagent

      Methanol



      1 × Transmembrane Buffer Components

      19.6 mM Tris-base, 150 mM Glycine, 20% (v/v) Methanol, pH8.3.

       

      Storage

      Store at room temperature for 6 months.

       

      Cautions

      1.This product does not provide methanol, but it is required.

      2.This product is a 10 × concentrate solution. Once it is formulated as a 1 × working solution and methanol is added, please use it within one week.

      3.This product does not contain SDS. For proteins with a molecular weight larger than 120 kDa or more hydrophobic, (0.025-0.1%) SDS can be added to prevent protein from accumulating in the gel.

      4.This product is a high concentrate. When the temperature is low, crystals may be precipitated. It can be heated to dissolve. Dilute after it is completely dissolved.

      5.Keep the product sealed to prevent from pollution.

      6.For your safety and health, please wear the lab coat and disposable gloves before the experiments.

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