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Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)

Citations(55)

  • Cat.No.:E-BC-K261-M

  • Detection instrument: Microplate reader (510 nm)

Manual MSDS

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Detection principle

Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of 4-aminoantipyrine and phenol, hydrogen peroxide is catalyzed by peroxidase (POD) to produce quinones which is proportional to the content of TG.

Performance characteristics

Synonyms TG
Sample type serum, plasma and tissue
Sensitivity 0.14 mmol/L
Detection range 0.14-10 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 9.200%Average intra-assay CV: 4.100%
Other instruments required Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Isopropanol(AR)，Anhydrous ethanol
Storage 2-8℃
Valid period 12 months

Images

Triglyceride level in cells were significantly reduced by atorvastatin treatment. (***P<0.001)

Y Gao et al developed a lipopeptide nanoplexes for atorvastatin transformation. Triglyceride (TG) level in mouse B16-F10 and CT-26 cell was determined using TG colorimetric assay kit (E-BC-K261-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.14-10 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Mouse serum

1

Rat plasma

1

10% Mouse liver tissue homogenate

1

10% Mouse kidney tissue homogenate

1

10% Mouse heart tissue homogenate

1

Note:   The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) for serum (plasma) samples;   The diluent is anhydrous ethanol for tissue samples.

Citations

  1. AMERICAN JOURNAL OF PATHOLOGY (2022) IF: 5.77
    Paraoxonase 1 Ameliorates Renal Lipotoxicity by Activating Lipophagy and Inhibiting Pyroptosis

    DOI: 10.1016/j.ajpath.2022.07.011

    PMID: 35963464

    		 Sample: Plasma
  2. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Integrating metabolomics and network pharmacology to investigate Panax japonicus prevents kidney injury in HFD/STZ-induced diabetic mice

    DOI: 10.1016/j.jep.2022.115893
    Sample: Serum
  3. Frontiers in Endocrinology (2022) IF: 6.055
    Total Flavonoids of Drynariae Rhizoma Improve Glucocorticoid-Induced Osteoporosis of Rats: UHPLC-MS-Based Qualitative Analysis, Network Pharmacology Strategy and Pharmacodynamic Validation

    DOI: 10.3389/fendo.2022.920931
    Sample: Serum
  4. Advanced Science (2023) IF: 17.521
    Antibody WB Operation Guide Video

    DOI: 10.1002/advs.202204909

    PMID: 36808719

    		 Sample: Serum
  5. FASEB JOURNAL (2021) IF: 5.192
    Holdemanella biformis improves glucose tolerance and regulates GLP-1 signaling in obese mice

    DOI: 10.1096/fj.202100126R

    PMID: 34143451

    		 Sample: Blood
  6. Reports of Biochemistry and Molecular Biology (2021)
    The Impact of Intermittent Hypobaric Hypoxia Exposures on Triacylglycerol Synthesis in Rat Liver

    DOI: 10.52547/rbmb.10.3.437
    Sample: Liver Tissue
  7. Vascular Health and Risk Management (2023)
    The Effectiveness of Nigella sativa and Ginger as Appetite Suppressants: An Experimental Study on Healthy Wistar Rats

    DOI: 10.2147/VHRM.S396295
    Sample: Serum
  8. Heliyon (2021)
    Phyllanthus amarus attenuated derangement in renal-cardiac function, redox status, lipid profile and reduced TNF-α, interleukins-2, 6 and 8 in high salt diet fed rats

    DOI: 10.1016/j.heliyon.2021.e08106
    Sample: Serum
  9. Journal of Advanced Pharmaceutical Technology & Research (2020)
    Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic rats

    DOI: 10.4103/japtr.JAPTR_28_20
    Sample: Serum
  10. Revista Brasileira de Medicina do Esporte (2022) IF: 0.652
    IMPACTS OF AEROBIC EXERCISE ON THE OBESITY OF ADOLESCENTS AND THEIR LIPID METABOLISM

    DOI: 10.1590/1517-8692202329012022_0163
    Sample: Serum
  11. International Journal of Clinical and Experimental Pathology (2018) IF: 1.396
    Pitavastatin attenuates atherosclerosis by suppressing NF-κB signaling in a high-cholesterol diet plus balloon catheter injury rabbit model

    PMID: 31938118

    		 Sample: Serum
  12. TOXICOLOGY AND INDUSTRIAL HEALTH (2020) IF: 1.708
    Elevated, sustained, and yet reversible biotoxicity effects of lead on cessation of exposure: Melatonin is a potent therapeutic option:

    DOI: 10.1177/0748233720937199
    Sample: Plasma
  13. JOURNAL OF BIOSCIENCES (2021) IF: 1.826
    Triglyceride regulate ACE2 level through MTHFD1

    DOI: 10.1007/s12038-021-00178-y

    PMID: 34168100

    		 Sample: Calu3 Cells
  14. AQUACULTURE RESEARCH (2021) IF: 2.082
    Quillaja saponaria and/or Yucca schidigera ameliorate water quality, growth performance, blood health, intestine and gills histomorphology of Nile tilapia, Oreochromis niloticus

    DOI: 10.1111/are.15474
    Sample: Serum
  15. Journal of Natural Medicines (2021) IF: 2.343
    Dehydroabietic acid improves nonalcoholic fatty liver disease through activating the Keap1/Nrf2-ARE signaling pathway to reduce ferroptosis

    DOI: 10.1007/s11418-021-01491-4

    PMID: 33590347

    		 Sample: Tissue Homogenate(Liver)
  16. Journal of Developmental Origins of Health and Disease (2020) IF: 2.456
    Neonatal zingerone protects against the development of high-fructose diet-induced metabolic syndrome in adult Sprague-Dawley rats

    DOI: 10.1017/S2040174420000525
    Sample: Plasma
  17. Dose-Response (2022) IF: 2.623
    Biochemical Investigation of Therapeutic Potentials of Plant-Based Bioactive Compounds as Stimulators of Glucagon like peptide-1 Secretion :

    DOI: 10.1177/15593258221114184
    Sample: Serum
  18. NUTRITION (2016) IF: 2.839
    Dietary sweet cherry anthocyanins attenuates diet-induced hepatic steatosis by improving hepatic lipid metabolism in mice

    DOI: 10.1016/j.nut.2016.01.007
    Sample: Liver Tissue
  19. MOLECULAR GENETICS AND GENOMICS (2023) IF: 2.98
    Regulation of PPARγ/CPT-1 expression ameliorates cochlear hair cell injury by regulating cellular lipid metabolism and oxidative stress

    DOI: 10.1007/s00438-023-01993-8
    Sample: House Ear Institute-Organ Of Corti 1 Cells (Hei-Oc
  20. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2022) IF: 3.168
    Serum Levels of Free Fatty Acids in Obese Mice and Their Associations with Routine Lipid Profiles

    DOI: 10.2147/DMSO.S348800

    PMID: 35140491

    		 Sample: Serum
  21. ANIMAL FEED SCIENCE AND TECHNOLOGY (2021) IF: 3.247
    Coating beef tallow with calcium soap improves dietary pellet quality and promotes an effective diet-induced obese mouse model

    DOI: 10.1016/j.anifeedsci.2021.115008
    Sample: Plasma
  22. EUROPEAN JOURNAL OF PHARMACOLOGY (2020) IF: 3.263
    Pancreastatin inhibitor PSTi8 protects the obesity associated skeletal muscle insulin resistance in diet induced streptozotocin-treated diabetic mice

    DOI: 10.1016/j.ejphar.2020.173204
    Sample: Plasma
  23. JOURNAL OF EXPERIMENTAL BIOLOGY (2021) IF: 3.312
    Monoterpenes alter TAR1-driven physiology in Drosophila species

    DOI: 10.1242/jeb.232116
  24. MEAT SCIENCE (2019) IF: 3.483
    Effects of naturally-produced lovastatin on carcass characteristics, muscle physico-chemical properties and lipid oxidation and cholesterol content in goats

    DOI: 10.1016/j.meatsci.2019.04.008
    Sample: Serum
  25. Nutrients (2017) IF: 3.55
    Flavonoid-Rich Extract of Paulownia fortunei Flowers Attenuates Diet-Induced Hyperlipidemia, Hepatic Steatosis and Insulin Resistance in Obesity Mice by AMPK Pathway

    DOI: 10.3390/nu9090959
    Sample: Liver
  26. MOLECULAR AND CELLULAR ENDOCRINOLOGY (2018) IF: 3.563
    Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice

    DOI: 10.1016/j.mce.2018.02.018
    Sample: Liver
  27. JOURNAL OF APPLIED TOXICOLOGY (2022) IF: 3.628
    Lanthanum nitrate inhibits adipogenesis in 3T3-L1 preadipocytes with a disorder of mitotic clonal expansion process

    DOI: 10.1002/jat.4391

    PMID: 36065135

    		 Sample: 3T3-L1 Preadipocyte
  28. LIFE SCIENCES (2020) IF: 3.647
    Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis

    DOI: 10.1016/j.lfs.2020.118118
    Sample: Serum
  29. JOURNAL OF PHARMACY AND PHARMACOLOGY (2021) IF: 3.765
    Cissus quadrangularis extract attenuates diabetic nephropathy by altering SIRT1/DNMT1 axis

    DOI: 10.1093/jpp/rgab078

    PMID: 34128987

    		 Sample: Plasma
  30. PEPTIDES (2022) IF: 3.867
    Pancreastatin inhibitor PSTi8 balances energy homeostasis by attenuating adipose tissue inflammation in high fat diet fed mice

    DOI: 10.1016/j.peptides.2022.170902
    Sample: Serum
  31. Scientific Reports (2020) IF: 3.998
    Disordered metabolism in mice lacking irisin

    DOI: 10.1038/s41598-020-74588-7

    PMID: 33060792

    		 Sample: Plasma
  32. ACS Omega (2022) IF: 4.132
    Beneficial Effects of lncRNA-UC.360+ shRNA on Diabetic Cardiac Sympathetic Damage via NLRP3 Inflammasome-Induced Pyroptosis in Stellate Ganglion

    DOI: 10.1021/acsomega.2c03619

    PMID: 35967043

    		 Sample: Serum
  33. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Biochemical investigation of human exposure to aflatoxin M1 and its association with risk factors of diabetes mellitus

    DOI: 10.1007/s11356-021-14871-w

    PMID: 34216342

    		 Sample: Serum
  34. Journal of Diabetes Investigation (2021) IF: 4.232
    Diet-induced prediabetes: Effects on the activity of the renin-angiotensin-aldosterone system (RAAS) in selected organs

    DOI: 10.1111/jdi.13690
    Sample: Liver
  35. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Zexie Tang targeting FKBP38/mTOR/SREBPs pathway improves hyperlipidemia

    DOI: 10.1016/j.jep.2022.115101

    PMID: 35151834

    		 Sample: Serum
  36. TOXICOLOGY LETTERS (2021) IF: 4.374
    Alpha-lipoic acid attenuates silica-induced pulmonary fibrosis by improving mitochondrial function via AMPK/PGC1α pathway activation in C57BL/6J mice

    DOI: 10.1016/j.toxlet.2021.07.003

    PMID: 34252510

    		 Sample: Serum
  37. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
    Isoliquiritigenin ameliorates caerulein‐induced chronic pancreatitis by inhibiting the activation of PSCs and pancreatic infiltration of macrophages

    DOI: 10.1111/jcmm.15498
    Sample: Serum
  38. JOURNAL OF PSYCHIATRIC RESEARCH (2022) IF: 4.791
    Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression

    DOI: 10.1016/j.jpsychires.2022.04.002

    PMID: 35429740

    		 Sample: Serum
  39. LIFE SCIENCES (2022) IF: 5.037
    sSTEAP4 regulates cellular homeostasis and improves high-fat-diet-caused oxidative stress in hepatocytes

    DOI: 10.1016/j.lfs.2022.120438

    PMID: 35227772

    		 Sample: Serum
  40. MOLECULAR NUTRITION & FOOD RESEARCH (2020) IF: 5.309
    Gymnaster Koraiensis Extract Alleviated Metabolic Syndrome Symptoms and Stimulated UCP1‐Independent Energy Consumption via AMPK Activation in White Adipose Tissue

    DOI: 10.1002/mnfr.202000490
    Sample: Serum
  41. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079
    Sample: Serum
  42. ENVIRONMENTAL MICROBIOLOGY (2022) IF: 5.491
    An inactivating mutation in the vacuolar arginine exporter gene Vae results in culture degeneration in the fungus Metarhizium robertsii

    DOI: 10.1111/1462-2920.15982

    PMID: 35352870
  43. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    		 Sample: Fecal,Serum
  44. Nutrients (2022) IF: 5.719
    The Allium Derivate Propyl Propane Thiosulfinate Exerts Anti-Obesogenic Effects in a Murine Model of Diet-Induced Obesity

    DOI: 10.3390/nu14030440

    PMID: 35276798

    		 Sample: Plasma
  45. Frontiers in Pharmacology (2021) IF: 5.811
    Metformin Alleviates Hepatic Steatosis and Insulin Resistance in a Mouse Model of High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease by Promoting Transcription Factor EB-Dependent Autophagy

    DOI: 10.3389/fphar.2021.689111
    Sample: Serum
  46. Food & Function (2023) IF: 6.317
    Analysis of the mechanism of action of quercetin in the treatment of hyperlipidemia based on metabolomics and intestinal flora

    DOI: 10.1039/D2FO03509J
    Sample: Serum
  47. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2021) IF: 6.514
    Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms

    DOI: 10.1016/j.ejmech.2021.113388

    PMID: 33784603

    		 Sample: Serum
  48. Frontiers in Cell and Developmental Biology (2021) IF: 6.684
    N6-Methyladenosine Methyltransferase METTL3 Promotes Angiogenesis and Atherosclerosis by Upregulating the JAK2/STAT3 Pathway via m6A Reader IGF2BP1.

    DOI: 10.3389/fcell.2021.731810
    Sample: Plasma
  49. BMC BIOLOGY (2022) IF: 7.431
    Histone acetyltransferase NAA40 modulates acetyl-CoA levels and lipid synthesis

    DOI: 10.1186/s12915-021-01225-8

    PMID: 35057804

    		 Sample: L3 Larvae
  50. PHARMACOLOGICAL RESEARCH (2019) IF: 7.658
    Dasatinib ameliorates chronic pancreatitis induced by caerulein via anti-fibrotic and anti-inflammatory mechanism

    DOI: 10.1016/j.phrs.2019.104357

    PMID: 31356863

    		 Sample: Serum
  51. SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
    Polystyrene microplastics induce hepatotoxicity and disrupt lipid metabolism in the liver organoids

    DOI: 10.1016/j.scitotenv.2021.150328

    PMID: 34571217

    		 Sample: Liver Organoids (Los)
  52. Cell Reports (2021) IF: 9.423
    Microenvironmental Stiffness Regulates Cellular Triglyceride Metabolism

    DOI: 10.2139/ssrn.3876697
    Sample: Chondrocytes
  53. SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 10.753
    Combined effect of polystyrene microplastics and bisphenol A on the human embryonic stem cells-derived liver organoids: The hepatotoxicity and lipid accumulation

    DOI: 10.1016/j.scitotenv.2022.158585

    PMID: 36089014

    		 Sample: Liver Organoids (Los)
  54. BIOMATERIALS (2022) IF: 15.304
    Self-amplified ROS production from fatty acid oxidation enhanced tumor immunotherapy by atorvastatin/PD-L1 siRNA lipopeptide nanoplexes

    DOI: 10.1016/j.biomaterials.2022.121902
    Sample: B16-F10 Cell,Ct-26 Cell
  55. Nature Communications (2022) IF: 17.694
    Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease

    DOI: 10.1038/s41467-022-35481-1

    PMID: 36572670

    		 Sample: Liver Tissue
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

M*****iSubmitted [ Oct 31 2022 ]

Asked: How to store cell samples?

Reply

A

adminSubmitted [ Oct 31 2022 ]

Answered: The cell samples (without homogenization) can be stored at -80℃ for a month.

Q

M*****iSubmitted [ Sep 29 2022 ]

Asked: Why is there a significant difference between the parallel holes？

Reply

A

adminSubmitted [ Sep 29 2022 ]

Answered: Probably because that there were bubbles in the wells. You can use a micropipettor to remove the bubbles. If you are not sure about what to do, please chat with our technical support for details.

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