High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents) (E-BC-K221-M)
For research use only.
| Detection Principle | CM, VLDL and LDL coagulate in a polyanionic environment to form a polymer and are masked by the polymer. High-density lipoprotein (HDL) form soluble compounds under the action of a surfactant, so that HDL-C can directly react with enzyme reagents containing cholesterol esterase (CE) and cholesterol oxidase (CO) to produce hydrogen peroxide. Hydrogen peroxide is catalyzed by peroxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound,the shade is proportional to the amount of HDL-C. |
| Synonyms | HDL-C |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-570 nm, optimum wavelength: 546 nm) |
| Research Area | Metabolic Diseases , Glycolysis And Lipid Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.06 mmol/L |
| Detection Range | 0.06-3.8 mmol/L |
| Precision | inter-assay CV: 0.05 | intra-assay CV: 0.03 |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Porcine serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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