Trypsin Activity Assay Kit (E-BC-K843-M)
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For research use only.
| Detection Principle | Trypsin catalyzes the substrate to form p-nitroaniline, which has a certain light absorption value at 400-410 nm wavelength. Since the absorbance of p-NA is proportional to the content, the activity of trypsin can be calculated by measuring the p-NA produced per unit time. |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-410 nm, optimum wavelength: 405 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.86 U/L |
| Detection Range | 1.86-100.65 U/L |
| Precision | inter-assay CV: 7.2-11.2% | intra-assay CV: 3.9-5.1% |
| Sample Volume | 20 μL(cell homogenate) |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse small intestine tissue homogenate | 2-5 |
| 10% Mouse large intestine tissue homogenate | 2-5 |
| 10% Rat intestine tissue homogenate | 2-5 |
| 10% Rat chyme tissue homogenate | 2-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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