Tyrosinase Activity Colorimetric Assay Kit (E-BC-K1402-M)
For research use only.
| Detection Principle |
Tyrosinase is widely distributed in higher animals and plants, primarily responsible for the browning of vegetables, beverages, and fruits, as well as the production of melanin in animals. Tyrosinase catalyzes the conversion of dopamine to dopaquinone. Dopaquinone forms a pink complex with MBTH. The tyrosinase activity in the sample can be calculated by measuring the rate of increase in OD value at 505 nm. |
| Sample Type | Serum,Plasma,Animal tissue,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-510 nm) |
| Other Reagents Required | DMSO, PBS（0.01 M，pH 7.4） |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.16 U/L |
| Detection Range | 0.16-15.57 U/L |
| Precision | inter-assay CV: 6.4%-8.1% | intra-assay CV: 0.4%-0.7% |
| Sample Volume | 20 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenization | 1 |
| 10% Mouse kidney tissue homogenization | 1 |
| Human serum | 1 |
| 10% sweet potato tissue homogenization | 2-10 |
| 10% potato tissue | 5-20 homogenization |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
No files available. Please enter the correct identification code in the search area to query the relevant documents.
| Title | Status | Valid until | Action | |
|---|---|---|---|---|
| {{v.文件名}} | {{v.status_str}} | {{v.timestamp_str}} | Download | / |
*Note：Please download the relevant files within the validity period. After expiration, file preview and download will not be available on the page.
Contact with Us
Houston, Texas, 77079, USA
