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Uncoated Human C5a(Complement Component 5a) ELISA Kit

Uniprot : P01031
  • Cat.No.:E-UNEL-H0022

  • Reactivity: Human

To Purchase E-UNEL-H0022

Size:
  • 96T*5
  • 96T*15
Price: $399$359.1
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Application

The new series of Elabscience® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

For in vitro quantitative determination of C5a concentration in Human serum, plasma.

Properties

Assay type Sandwich
Format 96T*5  96T*15
Assay time 24h
Reactivity Human
Storage -20℃.Please refer to the instructions for details.
Sample type serum, plasma
Sample volume 100μL

Kit components

Item Specifications Storage
Micro ELISA pre-Plate(Dismountable) 96T*5:5 plates, 96T -20℃, 6 months
Reference Standard 96T*5:1 vial
Concentrated Capture Ab 96T*5:1 vial 120μL
Concentrated Biotinylated Detection Ab 96T*5:1 vial 120μL
Concentrated HRP Conjugate 96T*5:1 vial 120μL -20℃(Protect from light), 6 months
Product Description 1 copy /
Certificate of Analysis 1 copy

Test Principle

1. Analyte-specific antibody (capture antibody) is bound to a pre-microplate, unbound capture antibody is washed away. Then plates are blocked.

2. Samples or standards are added and any analyte present is bound by the immobilized capture antibody. Unbound materials are decanted.

3. The added Biotinylated Detection Ab will bind to the analyte been captured by the immobilized antibody. Unbound Biotinylated Detection Ab is washed away.

4. HPR Conjugate is used to bind to the Biotinylated Detection Ab. Unbound HPR Conjugate is washed away.

5. TMB substrate solution is added to the wells and a blue color develops in proportion to the amount of analyte present in the sample. Then the Stop Solution is added to stop the color change of substrate, and the color inside the well changes from blue to yellow. The absorbance of the color at 450 nm is measured.

Citations

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