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Uric Acid (UA) Colorimetric Assay Kit

Name: Uric Acid (UA) Colorimetric Assay Kit
Brand: Elabscience
SKU: E-BC-K016-S
Price: 180 USD
Availability: InStock
Rating: 5 (1 reviews)

Citations(3)

Cat.No.:E-BC-K016-S
Detection instrument: Spectrophotometer(690 nm)

Detection method: Colorimetric method

Manual MSDS

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Detection principle

Uric acid can be used as an antioxidant to remove peroxide, hydroxyl and oxygen free radicals, chelate and transfer metal ions, protect vascular endothelial cells from damage. Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid.

Performance characteristics

Synonyms	UA
Sample type	Serum,plasma,urine
Sensitivity	0.58 mg/L
Detection range	0.58-100 mg/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 2.600%Average intra-assay CV: 1.800%
Other instruments required	Vortex mixer, Micropipettor, Centrifuge
Other reagents required	Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

Plasma UA shows no significant differences with different diets (P<0.05).

N A M Omar et al investigate the effect of high intakes of fructose and galactose, with or without added fructooligosaccharides (FOS), on metabolism factors, inflammation, and gut integrity markers in rats. Uric acid level in rat plasma was determined using UA colorimetric assay kit (E-BC-K016-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-100 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human urine	8-10
Human serum	1
Dog serum	1
Rat serum	1
Mouse serum	1-2
Porcine serum	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

DOI: 10.3390/ijms232315400

Sample: plasma,urine
Frontiers in Pharmacology (2021) IF: 5.811
Pharmacological Basis for Use of a Novel Compound in Hyperuricemia: Anti-Hyperuricemic and Anti-Inflammatory Effects.

DOI: 10.3389/fphar.2021.772504

Sample: Serum
Drug Design Development and Therapy (2021) IF: 4.162
Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways

DOI: 10.2147/DDDT.S326975

PMID: 34703210
Sample: serum

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Verified Customer

S***ySubmitted [ May 29 2019 ]

Application:Biochemical
Description:The kit is generally good, easy and fast,My linear correlation coefficient of the standard curve is not to reach 0.99

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