Urea (BUN) Colorimetric Assay Kit (Urease Method) (E-BC-K183-S)
For research use only.
| Detection Principle | Urea can be decomposed into ammonia ion and carbon dioxide by urease. Ammonia ion can react with phenol chromogenic agent and form a blue substance in alkaline medium, and the production of the blue substance is proportional to the urea content which can be calculated with the colorimetric assay at 580 nm. |
| Synonyms | BUN |
| Sample Type | Serum,Plasma,Urine,Saliva,Milk |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (580 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.114 mmol/L |
| Detection Range | 0.114-30 mmol/L |
| Precision | inter-assay CV: 4.7% | intra-assay CV: 4.6% |
| Sample Volume | 40 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Rabbit plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Human serum | 1 |
| Human saliva | 1 |
| Human milk | 1 |
| Human urine | 30-60 |
| Mouse urine | 30-60 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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