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Uric Acid (UA) Colorimetric Assay Kit

  • Cat.No.:E-BC-K016-M

  • Detection instrument: Microplate reader(680-700 nm,optimum wavelength: 690 nm)

To Purchase E-BC-K016-M

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  • 96T
  • 48T
Price: $180
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Detection principle

Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid. Uric acid content can be calculated by measuring the OD value at 690 nm.

Performance characteristics

Synonyms UA
Sample type Serum,plasma,urine
Sensitivity 1.30 mg/L
Detection range 1.30-80 mg/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 35 min
Precision Average inter-assay CV: 4%Average intra-assay CV: 2%
Other instruments required Micropipettor, Centrifuge, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 3 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.30-80 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Mouse serum

1-2

Rat serum

1

Human serum

1

Porcine serum

1

Dog serum

1-2

Human urine

8-10

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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