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Uric Acid (UA) Colorimetric Assay Kit

  • Cat.No.:E-BC-K016-M

  • Detection instrument: Microplate reader(680-700 nm,optimum wavelength: 690 nm)

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Price: $180

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Detection principle

Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid. Uric acid content can be calculated by measuring the OD value at 690 nm.

Performance characteristics

Synonyms UA
Sample type Serum,plasma,urine
Sensitivity 1.30 mg/L
Detection range 1.30-80 mg/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 35 min
Precision Average inter-assay CV: 4%Average intra-assay CV: 2%
Other instruments required Micropipettor, Centrifuge, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 3 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.30-80 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Mouse serum


Rat serum


Human serum


Porcine serum


Dog serum


Human urine


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


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