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Uric Acid (UA) Fluorometric Assay Kit

  • Cat.No.:E-BC-F018

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

To Purchase E-BC-F018

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Price: $360

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Detection principle

Uricase catalyzes the decomposition of uric acid into allantoin, CO2 and H2O2. Under the action of peroxidase, H2O2 oxidizes the non-fluorescent probe into the fluorescent substance. By measuring the fluorescence value of the system, the corresponding uric acid content can be calculated.

Performance characteristics

Synonyms UA
Sample type Serum, plasma, urine, animal tissue
Sensitivity 0.03 μmol/L
Detection range 0.03-15 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 40 min
Precision Average inter-assay CV: 7.2%Average intra-assay CV: 1.5%
Other instruments required Micropipettor, Vortex mixer, Centrifuge
Storage -20℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.03-15 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Human urine


Human hydrothorax


Rat urine


Rabbit serum


Rat serum


Porcine serum


10% Rat liver tissue homogenate


10% Rat kidney tissue homogenate


10% Rat lung tissue homogenate


Note: The diluent is reagent 1.

Ralated product

Cat Number Product Name
E-BC-K016-M Uric Acid (UA) Colorimetric Assay Kit


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