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Uric Acid (UA) Fluorometric Assay Kit

  • Cat.No.:E-BC-F018

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

To Purchase E-BC-F018

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  • 96T
  • 48T
Price: $360
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Detection principle

Uricase catalyzes the decomposition of uric acid into allantoin, CO2 and H2O2. Under the action of peroxidase, H2O2 oxidizes the non-fluorescent probe into the fluorescent substance. By measuring the fluorescence value of the system, the corresponding uric acid content can be calculated.

Performance characteristics

Synonyms UA
Sample type Serum, plasma, urine, animal tissue
Sensitivity 0.03 μmol/L
Detection range 0.03-15 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 40 min
Precision Average inter-assay CV: 7.2%Average intra-assay CV: 1.5%
Other instruments required Micropipettor, Vortex mixer, Centrifuge
Storage -20℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.03-15 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

10-20

Human urine

80-100

Human hydrothorax

50-60

Rat urine

10-20

Rabbit serum

5-10

Rat serum

10-20

Porcine serum

1

10% Rat liver tissue homogenate

10-20

10% Rat kidney tissue homogenate

30-40

10% Rat lung tissue homogenate

10-20

Note: The diluent is reagent 1.

Ralated product

Cat Number Product Name
E-BC-K016-M Uric Acid (UA) Colorimetric Assay Kit

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

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