Current Position:Home>>Kits>>Metabolism Assays>> Vitamin E (VE) Colorimetric Assay Kit

Vitamin E (VE) Colorimetric Assay Kit

Cat.No.:E-BC-K033-S
Detection instrument: Spectrophotometer(533 nm)

Detection method: Colorimetric method

To Purchase E-BC-K033-S

Size:

100Assays
50Assays

Price: $200

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. VE content can be calculated by measuring the OD value at 532 nm.

Performance characteristics

Synonyms	VE
Sample type	Serum,plasma,tissue
Sensitivity	0.09 μg/mL
Detection range	0.09-40 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 4.900%Average intra-assay CV: 4.400%
Other instruments required	Micropipettor, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol,N-heptane
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.09-40 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
10% Mouse liver tissue homogenization	1
Rat serum	1
Rat plasma	1
10% Carrot tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Nutrients (2023) IF: 6.706
Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets

DOI: 10.3390/nu15040832

Sample: serum
LIFE SCIENCES (2020) IF: 3.647
Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

DOI: 10.1016/j.lfs.2020.117584

PMID: 32220623
Sample: Tissue homogenate
LIFE SCIENCES (2020) IF: 3.647
Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades

DOI: 10.1016/j.lfs.2020.118876

PMID: 33310035
Sample: tissue
Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

DOI: 10.1002/jez.2603

Sample: brain

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

D***********iSubmitted [ Jul 05 2023 ]

Asked: Hello, would it be possible to use this kit to measure the Vitamin E level in peripheral blood mononuclear cells? Should we do the cell lysis step before we measure it?

adminSubmitted [ Jul 05 2023 ]

Answered: Hello, this kit can not measure cells. This kit is suitable for the detection of animal serum (plasma), animal and plant tissue samples

A***********hSubmitted [ Aug 16 2022 ]

Asked: Hello, is it possible to use samples from plant seeds in this test? If so, what about the sample preparation procedure?

adminSubmitted [ Aug 16 2022 ]

Answered: This kit can test plant tissue. Pls refer to tissue samples preparations in the manual.

F*********zSubmitted [ Aug 12 2022 ]

Asked: how much cost the trial kit? We are also interested to determine the Vitamin D content in proteins. Therfore, we would like to use your ELISA kit. Thank you very much for your answer!

adminSubmitted [ Aug 12 2022 ]

Answered: Many thanks for your interest in our Biochemical product. Could you do customs clearance by yourself? If not, would you mind purchasing from our local distributor? Maybe it will be easier. And if you would like to purchase from distributor, you can contact customer02@elabscience.com

F*********zSubmitted [ Aug 08 2022 ]

Asked: Hallo, is it possible to determine the overall Vitamin E content in protein samples? Do you have a preparation procedure?

adminSubmitted [ Aug 08 2022 ]

Answered: For this product "E-BC-K033-S", we have not verified the protein samples, so we are not sure about the applicability. If you are interested in our box, you can use a trial kit of this product

... Show All Show Less

Cat. No	E-BC-K033-S
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha