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Cat.No.:E-BC-K033-S
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Protocols for metabolism assays
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Synonyms | VE |
Sample type | Serum,plasma,tissue |
Sensitivity | 0.09 μg/mL |
Detection range | 0.09-40 μg/mL |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 60 min |
Precision | Average inter-assay CV: 4.900%Average intra-assay CV: 4.400% |
Other instruments required | Micropipettor, Vortex mixer, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol,N-heptane |
Storage | 2-8℃ |
Valid period | 12 months |
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.09-40 μg/mL).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Human plasma |
1 |
10% Mouse liver tissue homogenization |
1 |
Rat serum |
1 |
Rat plasma |
1 |
10% Carrot tissue homogenization |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
DOI: 10.3390/nu15040832 |
|
Sample: serum |
PMID: 32220623 |
Sample: Tissue homogenate |
PMID: 33310035 |
Sample: tissue |
DOI: 10.1002/jez.2603 |
|
Sample: brain |
D***********iSubmitted [ Jul 05 2023 ]
Asked: Hello, would it be possible to use this kit to measure the Vitamin E level in peripheral blood mononuclear cells? Should we do the cell lysis step before we measure it?
Reply
adminSubmitted [ Jul 05 2023 ]
Answered: Hello, this kit can not measure cells. This kit is suitable for the detection of animal serum (plasma), animal and plant tissue samples
A***********hSubmitted [ Aug 16 2022 ]
Asked: Hello, is it possible to use samples from plant seeds in this test? If so, what about the sample preparation procedure?
Reply
adminSubmitted [ Aug 16 2022 ]
Answered: This kit can test plant tissue. Pls refer to tissue samples preparations in the manual.
F*********zSubmitted [ Aug 12 2022 ]
Asked: how much cost the trial kit? We are also interested to determine the Vitamin D content in proteins. Therfore, we would like to use your ELISA kit. Thank you very much for your answer!
Reply
adminSubmitted [ Aug 12 2022 ]
Answered: Many thanks for your interest in our Biochemical product. Could you do customs clearance by yourself? If not, would you mind purchasing from our local distributor? Maybe it will be easier. And if you would like to purchase from distributor, you can contact customer02@elabscience.com
F*********zSubmitted [ Aug 08 2022 ]
Asked: Hallo, is it possible to determine the overall Vitamin E content in protein samples? Do you have a preparation procedure?
Reply
adminSubmitted [ Aug 08 2022 ]
Answered: For this product "E-BC-K033-S", we have not verified the protein samples, so we are not sure about the applicability. If you are interested in our box, you can use a trial kit of this product