YWHAZ Polyclonal Antibody
- +1
Price: $ 530
Price: $ 320
Price: $ 200
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB;IHC
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:HeLa,A549,MCF-7,Mouse kidney,Mouse brain,Rat brain Verified Samples in IHC:Rat brain,Mouse brain |
Dilution |
WB 1:500-1:2000, IHC 1:50-1:200 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | A synthetic peptide of human YWHAZ (NP_001129171.1). |
Abbre | YWHAZ |
Synonyms | YWHAZ;14-3-3-zeta;HEL-S-3;HEL-S-93;HEL4;KCIP-1;YWHAD |
Swissprot | |
Calculated MW | 19kDa/27kDa |
Observed MW |
28kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes. |
Concentration | 1mg/mL |
Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method | Affinity purification |
Research Areas | Cancer; Cell Biology; Neuroscience; Signal Transduction; Tags and Cell Markers |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. |