2-NBDG Glucose Uptake Cell-Based Kit (E-CK-A441)
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For research use only.
| Detection Principle | 2-NBDG Reagent is a green fluorescently labeled deoxyglucose analog with the same transporters (GLUTs) and similar transport kinetics as D-glucose.It is allowed to be phosphorylated by hexokinase because of a modification at the C-2 position.When it enters the cell, it is phosphorylated by hexokinase at the C-6 position to produce 2-NBDG-6-phosphate (a fluorescent derivative). Thus the fluorescence intensity reflects the glucose uptake. |
| Detection Method | Fluorometric method, Flow cytometry |
| Sample Type | Cell samples |
| Assay Time | 2 hours |
| Detection Instrument | Fluorescence microscope;flow Cytometer |
| Ex/Em | 475/550 |
| Storage | This product can be stored for 1 year at -20°C |
| Expiration date | 12 months |
| Shipping | Ice bag |
| Cat.No. | Product Name | Clone No. |
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