2-step plus Poly-HRP Anti Mouse IgG Detection System (with DAB Solution) (E-IR-R216)
For research use only.
| Product Introduction |
2-step plus is a two-step immunohistochemical broad spectrum detection reagent. It polymerizes monovalent Fab fragments of secondary antibody and enzyme, which replaces the secondary antibody and tertiary antibody in traditional method, can directly amplify the binding signal of antibody-antigen. This method not only retains the specific binding ability of antibody with antigen, but also can effectively avoid space steric hindrance caused by excessive polymer molecules. Compared with the traditional SP three-step method, this kit has the characteristics of simple, rapid, high-sensitivity. This system abandons the using of biotin, so it can avoid background staining by endogenous biotin. It can be used in IHC, in which the primary antibody is monoclonal/polyclonal antibody derived from Mouse.
Diluent for DAB concentrated solution has been provided in this kit to avoid the influence of the different water acidity and alkalinity on the DAB Chromogenic Agent. |
| Applications | IHC |
| Storage | Store at 2~8°C, shading light. Avoid of freezing. Valid for 12 months. The reagents are valid within 6 months after opening. |
| Shipping | Biological ice pack at 4 ℃ |
| Cautions |
1. This product couldn’t be used for clinical diagnosis or treatment, food or medicine, and can’t be stored in residence. 2. For your safety and health, please wear the lab coat and disposable gloves before the experiments. |
Other Clones
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Other Formats
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor®405
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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